Mouse Anti-C2 Recombinant Antibody (CBFYC-0617) (CBMAB-C0671-FY)

Name: Mouse Anti-C2 Recombinant Antibody (CBFYC-0617)
SKU: CBMAB-C0671-FY
Rating: 5 (1 reviews)

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Published Data

CBMAB-C0671-FY in IF

a-d Representative immunofluorescence (IF) stainings (paraffin-embedded tissue) for C3b and CFB (a), C4b and C2 (b), MBL and IgG (c), and C1q and IgG (d) in co-localization with the glomerular basement membrane constituent collagen IV in biopsies from MN patients (n = 5) and controls (n = 4). The lower panels represent 5-fold enlargements of the boxed areas in the upper panels. Bars 50 µm. ...View More

Seifert, L., Zahner, G., Meyer-Schwesinger, C., Hickstein, N., Dehde, S., Wulf, S., ... & Tomas, N. M. (2023). The classical pathway triggers pathogenic complement activation in membranous nephropathy. Nature Communications, 14(1), 473.

Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

CBFYC-0617

Application

WB, IP, IF, E, P

Immunogen

Amino acids 204-503 mapping within an internal region of C2 of human origin.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

0.09% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

complement C2

Introduction

Component C2 is a serum glycoprotein that functions as part of the classical pathway of the complement system.

Entrez Gene ID

717

UniProt ID

P06681

Alternative Names

CO2; ARMD14

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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