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Mouse Anti-C9orf72 Recombinant Antibody (CBYY-C3138) (CBMAB-C4581-YY)


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Published Data
CBMAB-C4581-YY-1
Mouse Anti-C9orf72 Recombinant Antibody (CBYY-C3138) (CBMAB-C4581-YY) in WB
Western blot analysis shows a reduction in human C9orf72 protein expression in C9orf72 ALS/FTD iPSC-MG. The 54 kDa C9orf72 protein band and 42 kDa actin protein band were used as a loading control (control, n = 4 lines, including an isogenic control-4, n = 1-2 differentiation per line; C9orf72, n = 5 lines, n = 1-2 differentiation per line). Bone marrow-derived macrophages (BMDM) from a C9orf72 knockout mouse were used to validate the antibody used for the Western blot analysis. ...View More
Lorenzini, I., Alsop, E., Levy, J., Gittings, L. M., Lall, D., Rabichow, B. E., ... & Sattler, R. (2023). Moderate intrinsic phenotypic alterations in C9orf72 ALS/FTD iPSC-microglia despite the presence of C9orf72 pathological features. Frontiers in cellular neuroscience, 17, 1179796.
CBMAB-C4581-YY-2
Mouse Anti-C9orf72 Recombinant Antibody (CBYY-C3138) (CBMAB-C4581-YY) in WB
Distribution of markers across synaptic fractions in C57BL/6 mice. Synaptophysin (Syp); Post-Synaptic Density Protein 95 (PSD-95); TAR DNA Binding Protein 43 (TDP-43); Chromosome 9 open reading frame 72 (C9orf72); Smith-Magenis syndrome Chromosomal Region candidate gene 8 (Smcr8); Ras-related proteins Rab3a, Rab5, Rab11, Rab39b; Glutamate receptor 1 (GluR1); glutamate receptor 2 (GluR2); N-methyl-D-aspartic acid receptor 1 (NMDAR1). ...View More
Xiao, S., McKeever, P. M., Lau, A., & Robertson, J. (2019). Synaptic localization of C9orf72 regulates post-synaptic glutamate receptor 1 levels. Acta neuropathologica communications, 7, 1-13.
CBMAB-C4581-YY-3
Mouse Anti-C9orf72 Recombinant Antibody (CBYY-C3138) (CBMAB-C4581-YY) in Immunoblot, IF
C9orf72 and Rab39b are localized to post-synaptic densities. a Immunoblot analysis of affinity purified PSD-95 complexes from C9-WT mice. PSD = PSD-95 antibody; UB = unbound fraction; IgG = rabbit IgG; wash = last wash. Synaptophysin (Syp); Post-Synaptic Density Protein 95 (PSD95); TAR DNA Binding Protein 43 (TDP-43); Chromosome 9 open reading frame 72 (C9orf72); Smith-Magenis syndrome Chromosomal Region candidate gene 8 (Smcr8); Ras-related proteins Rab3a, Rab5, Rab11, Rab39b; Glutamate receptor 1 (GluR1); glutamate receptor 2 (GluR2); N-methyl-D-aspartic acid receptor 1 (NMDAR1). Glial fibrillary acid protein (Gfap); Neurofilament Light Chain (Nefl). b Low magnification immunofluorescent micrographs of C9orf72, PSD-95, and DAPI or C9orf72, Synaptoporin (Synpr), and DAPI in the dorsal hippocampus of C9-WT and C9-KO mice. Scale = 200 μm. c High magnification immunofluorescent micrographs of C9orf72 with either PSD-95, microtubule associated protein 2 (MAP2), Synpr, or Rab39b in the CA3 region of the hippocampus from C9-WT and C9-KO mice. Insets contain arrowheads which indicate co-detection of fluorescent signal between the red and green channels. Scale = 20 μm. ...View More
Xiao, S., McKeever, P. M., Lau, A., & Robertson, J. (2019). Synaptic localization of C9orf72 regulates post-synaptic glutamate receptor 1 levels. Acta neuropathologica communications, 7, 1-13.
CBMAB-C4581-YY-4
Mouse Anti-C9orf72 Recombinant Antibody (CBYY-C3138) (CBMAB-C4581-YY) in Immunoblot
Lysosomes were immunoprecipitated using anti-HA magnetic beads. Quantitative immunoblotting for the indicated proteins was performed for starting material (SM), purified lysosomes and control immunoprecipitates (anti-HA IP). ...View More
Laflamme, C., McKeever, P. M., Kumar, R., Schwartz, J., Kolahdouzan, M., Chen, C. X., ... & McPherson, P. S. (2019). Implementation of an antibody characterization procedure and application to the major ALS/FTD disease gene C9ORF72. Elife, 8, e48363.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBYY-C3138
Antibody Isotype
IgG2a
Application
WB, IF, P

Basic Information

Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human C9orf72.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IHC-P1:100-1:1,000
WB1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Preservative
None
Concentration
1 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
C9orf72-SMCR8 Complex Subunit
Entrez Gene ID
Human203228
Mouse73205
Rat103730681
UniProt ID
HumanQ96LT7
MouseQ6DFW0
RatQ66HC3
Alternative Names
Claudin 5; Transmembrane Protein Deleted In Velocardiofacial Syndrome; Transmembrane Protein Deleted In VCFS; TMDVCF; TMVCF; AWAL; Claudin-5; CPETRL1; BEC1;
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For research use only. Not intended for any clinical use.

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