Mouse Anti-CABP1 Recombinant Antibody (F5) (CBMAB-1257-CN)

Name: Mouse Anti-CABP1 Recombinant Antibody (F5)
SKU: CBMAB-1257-CN
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

Application

WB, IP, IF, E

Immunogen

Amino acids 317-353 mapping within an internal region of CaBP1 of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

0.09% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

calcium binding protein 1

Introduction

Calcium binding proteins are an important component of calcium mediated cellular signal transduction. This gene encodes a protein that belongs to a subfamily of calcium binding proteins which share similarity to calmodulin. The protein encoded by this gene regulates the gating of voltage-gated calcium ion channels. This protein also regulates calcium-dependent activity of inositol 1,4,5-triphosphate receptors, P/Q-type voltage-gated calcium channels, and transient receptor potential channel TRPC5. It also prevents NMDA receptor-induced cellular degeneration.

Entrez Gene ID

Human	9478
Mouse	29867

UniProt ID

Human	Q9NZU7
Mouse	Q9JLK7

Alternative Names

CALBRAIN; HCALB_BR

Function

Modulates calcium-dependent activity of inositol 1,4,5-triphosphate receptors (ITPRs)(PubMed:14570872).
Inhibits agonist-induced intracellular calcium signaling (PubMed:15980432).
Enhances inactivation and does not support calcium-dependent facilitation of voltage-dependent P/Q-type calcium channels (PubMed:11865310).
Causes calcium-dependent facilitation and inhibits inactivation of L-type calcium channels by binding to the same sites as calmodulin in the C-terminal domain of CACNA1C, but has an opposite effect on channel function (PubMed:15140941).
Suppresses the calcium-dependent inactivation of CACNA1D (By similarity).
Inhibits TRPC5 channels (PubMed:15895247).
Prevents NMDA receptor-induced cellular degeneration. Required for the normal transfer of light signals through the retina (By similarity).

Biological Process

Negative regulation of protein import into nucleus Source: UniProtKB
Response to stimulus Source: UniProtKB-KW
Visual perception Source: UniProtKB

Cellular Location

Golgi apparatus; Cytoskeleton; Cell membrane; Perinuclear region; Postsynaptic density. L-CaBP1 is associated most likely with the cytoskeletal structures, whereas S-CaBP1 is localized at or near the plasma membrane.
Isoform L-CaBP1: Cytoskeleton. L-CaBP1 is associated most likely with the cytoskeletal structures.
Isoform S-CaBP1: Cell membrane; Cell cortex. S-CaBP1 is localized at or near the plasma membrane.

PTM

Phosphorylated. The phosphorylation regulates the activity.

Yang, T., Choi, J. E., Soh, D., Tobin, K., Joiner, M. L., Hansen, M., & Lee, A. (2018). CaBP1 regulates Cav1 L-type Ca2+ channels and their coupling to neurite growth and gene transcription in mouse spiral ganglion neurons. Molecular and Cellular Neuroscience, 88, 342-352.

Yang, T., Britt, J. K., Cintrón-Pérez, C. J., Vázquez-Rosa, E., Tobin, K. V., Stalker, G., ... & Lee, A. (2018). Ca2+-binding protein 1 regulates hippocampal-dependent memory and synaptic plasticity. Neuroscience, 380, 90-102.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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