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Mouse Anti-CALB2 Recombinant Antibody (CBYY-C0876) (CBMAB-C2313-YY)

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Basic Information

Host Animal
Mouse
Clone
CBYY-C0876
Application
ELISA, WB, IP, IF, IHC-P
Immunogen
Amino acids 2-27 at the N-terminus of Calretinin of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG3, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA1:100-1:1,000
IF(ICC)1:50-1:500
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
Calbindin 2
Entrez Gene ID
Human794
Mouse12308
UniProt ID
HumanP22676
MouseQ08331
Alternative Names
Calcium/Calmodulin Dependent Protein Kinase I; CaM Kinase I Alpha; EC 2.7.11.17; CaMKI-Alpha; CaM-KI; Calcium/Calmodulin-Dependent Protein Kinase Type 1; CaM Kinase I; EC 2.7.11; CAMKI;
Function
Calretinin is a calcium-binding protein which is abundant in auditory neurons.
Biological Process
Regulation of cytosolic calcium ion concentration Source: GO_Central
Regulation of long-term synaptic potentiation Source: GO_Central
Regulation of presynaptic cytosolic calcium ion concentration Source: GO_Central
Cellular Location
Cuticular plate; Cytosol; Nucleus; Synaptic membrane; Dendrite; Gap junction; Neuron projection; Parallel fiber to Purkinje cell synapse; Stereocilium; Synapse; Terminal bouton

Xiang, Y., Wu, Y., Zhang, H., Wu, J., & Zhang, J. (2020). Characterization and Localization of Calb2 in Both the Testis and Ovary of the Japanese Flounder (Paralichthys olivaceus). Animals, 10(9), 1503.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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