Mouse Anti-CALCOCO2 Recombinant Antibody (CBWJN-1056) (CBMAB-N0640-WJ)

Name: Mouse Anti-CALCOCO2 Recombinant Antibody (CBWJN-1056)
SKU: CBMAB-N0640-WJ
Rating: 5 (1 reviews)

See all CALCOCO2 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBWJN-1056

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 147-446 mapping at the C-terminus of CALCOCO2 of human origin.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:100-1:1,000
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Calcium Binding And Coiled-Coil Domain 2

Introduction

This gene encodes a coiled-coil domain-containing protein. The encoded protein functions as a receptor for ubiquitin-coated bacteria and plays an important role in innate immunity by mediating macroautophagy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]

Entrez Gene ID

10241

UniProt ID

Q13137

Alternative Names

Calcium Binding And Coiled-Coil Domain 2; Antigen Nuclear Dot 52 KDa Protein; Nuclear Domain 10 Protein NDP52; Nuclear Domain 10 Protein 52; Nuclear Dot Protein 52; NDP52; Calcium-Binding And Coiled-Coil Domain-Containing Protein 2;

Function

Xenophagy-specific receptor required for autophagy-mediated intracellular bacteria degradation. Acts as an effector protein of galectin-sensed membrane damage that restricts the proliferation of infecting pathogens such as Salmonella typhimurium upon entry into the cytosol by targeting LGALS8-associated bacteria for autophagy (PubMed:22246324).
Initially orchestrates bacteria targeting to autophagosomes and subsequently ensures pathogen degradation by regulating pathogen-containing autophagosome maturation (PubMed:23022382, PubMed:25771791).
Bacteria targeting to autophagosomes relies on its interaction with MAP1LC3A, MAP1LC3B and/or GABARAPL2, whereas regulation of pathogen-containing autophagosome maturation requires the interaction with MAP3LC3C (PubMed:23022382, PubMed:25771791).
May play a role in ruffle formation and actin cytoskeleton organization and seems to negatively regulate constitutive secretion (PubMed:17635994).

Biological Process

Positive regulation of autophagosome maturation Source: GO_Central
Response to interferon-gamma Source: BHF-UCL
Viral process Source: ProtInc
Xenophagy Source: GO_Central

Cellular Location

Cytoskeleton; Perinuclear region; Autophagosome membrane. According to PubMed:7540613, localizes to nuclear dots. According to PubMed:9230084 and PubMed:12869526, it is not a nuclear dot-associated protein but localizes predominantly in the cytoplasm with a coarse-grained distribution preferentially close to the nucleus.

Cui, F., Wang, S., Tan, J., Tang, H., Fan, Y., & Hu, J. (2021). Calcium‑binding and coiled‑coil domain 2 promotes the proliferation and suppresses apoptosis of prostate cancer cells. Experimental and therapeutic medicine, 21(4), 1-9.

Yamano, K., & Youle, R. J. (2020). Two different axes CALCOCO2-RB1CC1 and OPTN-ATG9A initiate PRKN-mediated mitophagy. Autophagy, 16(11), 2105-2107.

Tian, Z., Ning, C., Fu, C., Xu, F., Zou, C., Zhu, Q., ... & Wang, Y. (2020). CALCOCO2 silencing represents a potential molecular therapeutic target for glioma. Archives of Medical Science.

Mohamud, Y., Qu, J., Xue, Y. C., Liu, H., Deng, H., & Luo, H. (2019). CALCOCO2/NDP52 and SQSTM1/p62 differentially regulate coxsackievirus B3 propagation. Cell Death & Differentiation, 26(6), 1062-1076.

Leymarie, O., Meyer, L., Tafforeau, L., Lotteau, V., Da Costa, B., Delmas, B., ... & Le Goffic, R. (2017). Influenza virus protein PB1-F2 interacts with CALCOCO2 (NDP52) to modulate innate immune response. Journal of General Virology, 98(6), 1196-1208.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-CALCOCO2 Recombinant Antibody (CBWJN-1056)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Antibody Pairs

CALCOCO2 Matched Antibody Pair (167) (CAT#: APMAB-167LY)

Related Products

Mouse Anti-CALCOCO2 Recombinant Antibody (CBFYC-0739) (CAT#: CBMAB-C0794-FY)

CALCOCO2 Matched Antibody Pair (167) (CAT#: APMAB-167LY)

Mouse Anti-CALCOCO2 Recombinant Antibody (1A11) (CAT#: CBMAB-C3508-LY)

Rabbit Anti-CALCOCO2 Recombinant Antibody (D1E4A) (CAT#: CBMAB-CP1658-LY)

Mouse Anti-CALCOCO2 Recombinant Antibody (CBWJN-1057) (CAT#: CBMAB-N0641-WJ)

Mouse Anti-CALCOCO2 Recombinant Antibody (3C4) (CAT#: CBMAB-C5808-LY)

Mouse Anti-CALCOCO2 Recombinant Antibody (CBFYC-0741) (CAT#: CBMAB-C0796-FY)

Mouse Anti-CALCOCO2 Recombinant Antibody (CBWJN-1058) (CAT#: CBMAB-N0642-WJ)

Mouse Anti-CALCOCO2 Recombinant Antibody (CBFYC-0740) (CAT#: CBMAB-C0795-FY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry