Mouse Anti-CAND2 Recombinant Antibody (CBYJT-1058) (CBMAB-T0068-YJ)

Name: Mouse Anti-CAND2 Recombinant Antibody (CBYJT-1058)
SKU: CBMAB-T0068-YJ
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Rat, Mouse

Clone

CBYJT-1058

Antibody Isotype

IgG1, κ

Application

WB, IP, IF, ELISA

Basic Information

Immunogen

Amino acids 726-748 within an internal region of TIP120B of human origin.

Host Species

Mouse

Specificity

Human, Rat, Mouse

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:100-1:1,000
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 726-748

Target

Full Name

cullin-associated and neddylation-dissociated 2 (putative)

Introduction

Diseases associated with CAND2 include Esophageal Atresia/Tracheoesophageal Fistula. Gene Ontology (GO) annotations related to this gene include binding and TBP-class protein binding. An important paralog of this gene is CAND1.

Entrez Gene ID

Human	23066
Mouse	67088
Rat	192226

UniProt ID

Human	O75155
Mouse	Q6ZQ73
Rat	Q9R0L4

Alternative Names

Cullin Associated And Neddylation Dissociated 2 (Putative); Cullin-Associated And Neddylation-Dissociated Protein 2; TBP-Interacting Protein Of 120 KDa B; Epididymis Tissue Protein Li 169; TBP-Interacting Protein 120B; TBP Interacting Protein; P120 CAND2

Function

Probable assembly factor of SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complexes that promotes the exchange of the substrate-recognition F-box subunit in SCF complexes, thereby playing a key role in the cellular repertoire of SCF complexes.

Biological Process

Positive regulation of transcription, DNA-templated Source: UniProtKB
Protein ubiquitination Source: GO_Central
SCF complex assembly Source: GO_Central

Cellular Location

Nucleus

PTM

Ubiquitinated and targeted for proteasomal degradation.

More Infomation

References

Gorska, A., Sandmann, C., Riechert, E., Hofmann, C., Malovrh, E., Varma, E., ... & Völkers, M. (2020). Muscle specific translational control of Cand2 by mTORC1 regulates adverse cardiac remodeling. bioRxiv.

Sandmann, C., Gorska, A., Hofmann, C., Katus, H., & Völkers, M. (2018). Cand2, a Translationally Regulated Muscle Specific Protein, Mediates mTORC1 Dependent Cardiac Hypertrophy. Circulation, 138(Suppl_1), A15125-A15125.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
Request for COA

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Second antibody and isotype control

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