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Mouse Anti-CAPN5 Recombinant Antibody (3C10) (CBMAB-C5774-LY)

This product is antibody recognizes CAPN5. The antibody 3C10 immunoassay techniques such as: ELISA, WB.
See all CAPN5 antibodies
Published Data
Fig.1 Western blot analyses of calpain-5 in the mitochondrial subfractions.
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Fig.2 Immunohistochemistry in the porcine retina. (a) Image of staining with the calpain-5 antibody (green).
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Fig.3 CAPN5 colocalizes with SLIT2 in 661W cells. Scale bar: 20 lm.
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Fig.4 CAPN5 associates with SLIT2 in the mouse retina. IP, immunoprecipitation.
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Fig.5 CAPN5 promotes proteolytic cleavage of SLIT2 in neuronal cells.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
3C10
Antibody Isotype
IgG2a, κ
Application
ELISA, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Calpain 5
Introduction
Calpains are calcium-dependent cysteine proteases involved in signal transduction in a variety of cellular processes. A functional calpain protein consists of an invariant small subunit and 1 of a family of large subunits. CAPN5 is one of the large subunits. Unlike some of the calpains, CAPN5 and CAPN6 lack a calmodulin-like domain IV. Because of the significant similarity to Caenorhabditis elegans sex determination gene tra-3, CAPN5 is also called as HTRA3. [provided by RefSeq, Jul 2008]
Entrez Gene ID
726
UniProt ID
O15484
Alternative Names
Calpain 5; Calpain Htra-3; New Calpain 3; NCL-3; Vitreoretinopathy, Neovascular Inflammatory; Testis Tissue Sperm-Binding Protein Li 91mP; EC 3.4.22.54; EC 3.4.22.-;
Function
Calcium-regulated non-lysosomal thiol-protease.
Biological Process
Proteolysis Source: GO_Central
Signal transduction Source: ProtInc
Cellular Location
Extracellular exosome; Cell surface; Cytoplasm; Focal adhesion
Involvement in disease
Vitreoretinopathy, neovascular inflammatory (VRNI): An autoimmune condition of the eye that sequentially mimics uveitis, retinitis pigmentosa, and proliferative diabetic retinopathy as it progresses to complete blindness. Patients present during the second or third decade of life with posterior uveitis and reduction of the electroretinogram b-wave. They become more symptomatic when cataracts, cystoid macular edema, and disk edema diminish visual acuity during the second stage. Severe vision loss begins during the third stage when proliferative retinal neovascularization and epiretinal membranes appear. There is an ongoing pigmentary retinal degeneration and peripheral visual field loss during all stages. In the fourth stage, proliferative vitreoretinopathy causes tractional retinal detachments at the macula and vitreous base. The fifth or end-stage disease is marked by phthisis.

Bondada, V., Gal, J., Mashburn, C., Rodgers, D. W., Larochelle, K. E., Croall, D. E., & Geddes, J. W. (2021). The C2 domain of calpain 5 contributes to enzyme activation and membrane localization. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1868(7), 119019.

Tang, P. H., Chemudupati, T., Wert, K. J., Folk, J. C., Mahajan, M., Tsang, S. H., ... & Mahajan, V. B. (2020). Phenotypic variance in Calpain-5 retinal degeneration. American journal of ophthalmology case reports, 18, 100627.

Wert, K. J., Koch, S. F., Velez, G., Hsu, C. W., Mahajan, M., Bassuk, A. G., ... & Mahajan, V. B. (2019). CAPN5 genetic inactivation phenotype supports therapeutic inhibition trials. Human mutation, 40(12), 2377-2392.

Randazzo, N. M., Shanks, M. E., Clouston, P., & MacLaren, R. E. (2019). Two novel CAPN5 variants associated with mild and severe autosomal dominant neovascular inflammatory vitreoretinopathy phenotypes. Ocular immunology and inflammation, 27(5), 693-698.

Coomer, C. E., & Morris, A. C. (2018). Capn5 expression in the healthy and regenerating zebrafish retina. Investigative ophthalmology & visual science, 59(8), 3643-3654.

Velez, G., Bassuk, A. G., Schaefer, K. A., Brooks, B., Gakhar, L., Mahajan, M., ... & Mahajan, V. B. (2018). A novel de novo CAPN5 mutation in a patient with inflammatory vitreoretinopathy, hearing loss, and developmental delay. Molecular Case Studies, 4(3), a002519.

Bruening, J., Lasswitz, L., Banse, P., Kahl, S., Marinach, C., Vondran, F. W., ... & Gerold, G. (2018). Hepatitis C virus enters liver cells using the CD81 receptor complex proteins calpain-5 and CBLB. PLoS pathogens, 14(7), e1007111.

Schaefer, K., Mahajan, M., Gore, A., Tsang, S. H., Bassuk, A. G., & Mahajan, V. B. (2017). Calpain-5 gene expression in the mouse eye and brain. BMC research notes, 10(1), 1-7.

Wang, Y., Zhang, X., Song, Z., & Gu, F. (2017). An anti-CAPN5 intracellular antibody acts as an inhibitor of CAPN5-mediated neuronal degeneration. Oncotarget, 8(59), 100312.

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For research use only. Not intended for any clinical use.

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