Mouse Anti-CARHSP1 Recombinant Antibody (C6964) (CBMAB-C6964-LY)

Name: Mouse Anti-CARHSP1 Recombinant Antibody (C6964)
SKU: CBMAB-C6964-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

C6964

Application

WB, ELISA, IP, IF

Immunogen

Amino acids 10-45 mapping near the N-terminus of CRHSP-24 of human origin.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:100-1:1,000
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

calcium regulated heat stable protein 1, 24kDa

Introduction

CARHSP1 (Calcium Regulated Heat Stable Protein 1) is a Protein Coding gene. Gene Ontology (GO) annotations related to this gene include nucleic acid binding and mRNA 3-UTR binding. An important paralog of this gene is CSDC2.

Entrez Gene ID

23589

UniProt ID

Q9Y2V2

Alternative Names

Calcium Regulated Heat Stable Protein 1; Calcium-Regulated Heat-Stable Protein Of 24 KDa; Calcium Regulated Heat Stable Protein 1, 24kDa; CRHSP-24; Calcium Regulated Heat Stable Protein 1 (24kD); Calcium-Regulated Heat-Stable Protein (24kD);

Function

Binds mRNA and regulates the stability of target mRNA. Binds single-stranded DNA (in vitro).

Biological Process

Intracellular signal transduction Source: ProtInc
Regulation of mRNA stability Source: UniProtKB

Cellular Location

Cytoplasm; P-body; Cytoplasmic granule. Detected at cytoplasmic stress granules and P-bodies. Detected at exosome granules where mRNA is degraded (By similarity).

PTM

Dephosphorylated by calcineurin in a Ca2+ dependent manner (By similarity). Can be phosphorylated by DYRK2 (in vitro).

Zhu, G. D., Yu, J., Sun, Z. Y., Chen, Y., Zheng, H. M., Lin, M. L., ... & Shao, F. M. (2021). Genome-wide CRISPR/Cas9 screening identifies CARHSP1 responsible for radiation resistance in glioblastoma. Cell death & disease, 12(8), 1-9.

Song, X., Yang, C., Chang, J., & Xue, X. (2020). Dual impacts of lncRNA XIST and lncRNA SNHG5 on inflammatory reaction and apoptosis of endothelial cells via regulating miR‐155/CARHSP1 axis. Journal of Cellular and Molecular Medicine, 24(22), 13248-13265.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-CARHSP1 Recombinant Antibody (CBXC-1611) (CAT#: CBMAB-C5906-CQ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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