Human Recombinant CARHSP1 protein, His Tag (V2LY-0526-LY2582)

Name: Human Recombinant CARHSP1 protein, His Tag
SKU: V2LY-0526-LY2582
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant CARHSP1 protein, His Tag consist of Amino Acid: 1-147 and predicts a molecular mass of 17.7 kDa.

Molecule Mass

17.7 kDa

Sequence

Amino Acid: 1-147

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>85% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tirs, NaCl, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

calcium regulated heat stable protein 1, 24kDa

Function

Binds mRNA and regulates the stability of target mRNA. Binds single-stranded DNA (in vitro).

Biological Process

Intracellular signal transduction Source: ProtInc
Regulation of mRNA stability Source: UniProtKB

Cellular Location

Cytoplasm; P-body; Cytoplasmic granule. Detected at cytoplasmic stress granules and P-bodies. Detected at exosome granules where mRNA is degraded (By similarity).

PTM

Dephosphorylated by calcineurin in a Ca2+ dependent manner (By similarity). Can be phosphorylated by DYRK2 (in vitro).

Zhu, G. D., Yu, J., Sun, Z. Y., Chen, Y., Zheng, H. M., Lin, M. L., ... & Shao, F. M. (2021). Genome-wide CRISPR/Cas9 screening identifies CARHSP1 responsible for radiation resistance in glioblastoma. Cell death & disease, 12(8), 1-9.

Song, X., Yang, C., Chang, J., & Xue, X. (2020). Dual impacts of lncRNA XIST and lncRNA SNHG5 on inflammatory reaction and apoptosis of endothelial cells via regulating miR‐155/CARHSP1 axis. Journal of Cellular and Molecular Medicine, 24(22), 13248-13265.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Mouse Anti-CARHSP1 Recombinant Antibody (C6964) (CAT#: CBMAB-C6964-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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