Rabbit Anti-CASP12 Recombinant Antibody (C2658) (CBMAB-C2658-LY)

Name: Rabbit Anti-CASP12 Recombinant Antibody (C2658)
SKU: CBMAB-C2658-LY
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Rabbit

Specificity

Mouse

Clone

C2658

Antibody Isotype

IgG

Application

Basic Information

Immunogen

Recombinant protein specific to a region within the large subunit of mouse Caspase-12 protein.

Host Species

Rabbit

Specificity

Mouse

Antibody Isotype

IgG

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

HEPES, pH 7.5, 100 µg/ml BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

Caspase 12 (Gene/Pseudogene)

Introduction

Caspases are cysteine proteases that cleave C-terminal aspartic acid residues on their substrate molecules. This gene is most highly related to members of the ICE subfamily of caspases that process inflammatory cytokines. In rodents, the homolog of this gene mediates apoptosis in response to endoplasmic reticulum stress. However, in humans this gene contains a polymorphism for the presence or absence of a premature stop codon. The majority of human individuals have the premature stop codon and produce a truncated non-functional protein. The read-through codon occurs primarily in individuals of African descent and carriers have endotoxin hypo-responsiveness and an increased susceptibility to severe sepsis. Several alternatively spliced transcript variants have been noted for this gene. [provided by RefSeq, Feb 2011]

Entrez Gene ID

12364

UniProt ID

O08736

Function

Has no protease activity. May reduce cytokine release in response to bacterial lipopolysaccharide during infections. Reduces activation of NF-kappa-B in response to TNF.

Biological Process

Execution phase of apoptosis Source: GOC
Regulation of apoptotic process Source: InterPro
Regulation of inflammatory response Source: GO_Central

Cellular Location

AIM2 inflammasome complex; IPAF inflammasome complex; NLRP3 inflammasome complex; Endoplasmic reticulum; Cytoplasm

More Infomation

References

Chow, S. E., Chien, H. T., Chu, W. K., Lin, V., Shen, T. H., & Huang, S. F. (2021). Human Caspase 12 Enhances NF-κB Activity through Activation of IKK in Nasopharyngeal Carcinoma Cells. International Journal of Molecular Sciences, 22(9), 4610.

Mamoor, S. (2020). CASP12 is differentially expressed in high-grade serous ovarian cancers.

Vesela, B., Kratochvilova, A., Svandova, E., Benes, P., Rihova, K., Poliard, A., & Matalova, E. (2020). Caspase-12 Is Present During Craniofacial Development and Participates in Regulation of Osteogenic Markers. Frontiers in cell and developmental biology, 8, 1090.

Feng, G., Beilei, Z., Caizhi, C., & Wen, Z. (2019). Analysis of CASP12 diagnostic and prognostic values in cervical cancer based on TCGA database. Bioscience reports, 39(12), BSR20192706.

Song, J., Zhang, Q., Wang, S., Yang, F., Chen, Z., Dong, Q., ... & Ren, D. (2018). Cleavage of caspase‐12 at Asp94, mediated by endoplasmic reticulum stress (ERS), contributes to stretch‐induced apoptosis of myoblasts. Journal of cellular physiology, 233(12), 9473-9487.

Chu, W. K., Hsu, C. C., Huang, S. F., Hsu, C. C., & Chow, S. E. (2017). Caspase 12 degrades IκBα protein and enhances MMP-9 expression in human nasopharyngeal carcinoma cell invasion. Oncotarget, 8(20), 33515.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

Mouse Anti-CASP12 Recombinant Antibody (CBXC-1797)

Rat Anti-CASP12 Recombinant Antibody (CBFYC-0825)

Rat Anti-CASP12 Recombinant Antibody (14F4)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
Request for COA

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Second antibody and isotype control

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