Mouse Anti-CASP5 Recombinant Antibody (C6948) (CBMAB-C6948-LY)

Name: Mouse Anti-CASP5 Recombinant Antibody (C6948)
SKU: CBMAB-C6948-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

C6948

Application

ELISA, IF, IP, WB

Immunogen

Amino acids 120-153 at the N-terminus of caspase-5 p20 of human origin.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgM, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:100-1:1,000
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Caspase 5

Introduction

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. Overexpression of the active form of this enzyme induces apoptosis in fibroblasts. Max, a central component of the Myc/Max/Mad transcription regulation network important for cell growth, differentiation, and apoptosis, is cleaved by this protein; this process requires Fas-mediated dephosphorylation of Max. The expression of this gene is regulated by interferon-gamma and lipopolysaccharide. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Aug 2010]

Entrez Gene ID

838

UniProt ID

P51878

Function

Thiol protease that acts as a mediator of programmed cell death (PubMed:29898893, PubMed:28314590).
Initiates pyroptosis, a programmed lytic cell death pathway through cleavage of Gasdermin-D (GSDMD): cleavage releases the N-terminal gasdermin moiety (Gasdermin-D, N-terminal) that binds to membranes and forms pores, triggering pyroptosis (PubMed:29898893).
During non-canonical inflammasome activation, cuts CGAS and may play a role in the regulation of antiviral innate immune activation (PubMed:28314590).

Biological Process

Apoptotic process Source: ProtInc
Cellular response to mechanical stimulus Source: UniProtKB
Execution phase of apoptosis Source: GOC
Proteolysis Source: ProtInc
Regulation of apoptotic process Source: InterPro
Regulation of inflammatory response Source: GO_Central
Substantia nigra development Source: UniProtKB

Cellular Location

NLRP1 inflammasome complex; Cytoplasm

PTM

The two subunits are derived from the precursor sequence by an autocatalytic mechanism.

Wang, X., Ni, J., You, Y., Feng, G., Zhang, S., Bao, W., ... & Shen, X. (2021). SNX10‐mediated LPS sensing causes intestinal barrier dysfunction via a caspase‐5‐dependent signaling cascade. The EMBO Journal, e108080.

Dickinson, M. S., & Coers, J. (2021). SNX10 and caspase‐5 sort out endosomal LPS for a gut‐wrenching Slug‐fest. The EMBO journal, e110128.

Alwan, A. H. (2020). DETECTION OF CASP-5 GENE AS INFLAMMATORY FACTOR IN IRAQI PATIENTS WITH PSEUDOMONAS AERUGINOSA INFECTIONS. Pakistan Journal of Biotechnology, 17(1), 33-40.

Wu, Y., Zhuang, J., Zhao, D., & Xu, C. (2019). Interaction between caspase‐3 and caspase‐5 in the stretch‐induced programmed cell death in the human periodontal ligament cells. Journal of cellular physiology, 234(8), 13571-13581.

Rui, H., Yan, T., Hu, Z., Liu, R., & Wang, L. (2018). The association between caspase-5 gene polymorphisms and rheumatoid arthritis in a Chinese population. Gene, 642, 307-312.

Bitto, N. J., Baker, P. J., Dowling, J. K., Wray‐McCann, G., De Paoli, A., Tran, L. S., ... & Ferrero, R. L. (2018). Membrane vesicles from Pseudomonas aeruginosa activate the noncanonical inflammasome through caspase‐5 in human monocytes. Immunology and cell biology, 96(10), 1120-1130.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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