Mouse Anti-CASP6 Recombinant Antibody (6CSP03) (CBMAB-C8142-LY)

Name: Mouse Anti-CASP6 Recombinant Antibody (6CSP03)
SKU: CBMAB-C8142-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

6CSP03

Application

IP, WB

Immunogen

The prodomain of caspase-6 of human origin.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Caspase 6

Introduction

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family of enzymes. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic acid residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspases 7, 8 and 10, and is thought to function as a downstream enzyme in the caspase activation cascade. Alternative splicing of this gene results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Oct 2015]

Entrez Gene ID

839

UniProt ID

P55212

Alternative Names

Caspase 6; Caspase 6, Apoptosis-Related Cysteine Peptidase; Caspase 6, Apoptosis-Related Cysteine Protease; EC 3.4.22.59; MCH2; Apoptotic Protease MCH-2;

Function

Cysteine protease that plays essential roles in programmed cell death, axonal degeneration, development and innate immunity (PubMed:8663580, PubMed:32298652).
During apoptosis, localizes in the nucleus and cleaves the nuclear structural protein NUMA1 and lamin A/LMNA thereby inducing nuclear shrinkage and fragmentation (PubMed:17401638, PubMed:8663580, PubMed:9463409).
Furthermore, cleaves many transcription factors such as NF-kappa-B and cAMP response element-binding protein/CREBBP (PubMed:10559921, PubMed:14657026).
Plays an essential role in axon degeneration during axon pruning which is the remodeling of axons during neurogenesis but not apoptosis (By similarity).
Regulates B-cell programs both during early development and after antigen stimulation (By similarity).
In addition, promotes the ZBP1-mediated activation of programmed cell death pathways including pyroptosis, apoptosis, and necroptosis (PANoptosis) and plays an essential role in defense against viruses (PubMed:32298652).
Mechanistically, interacts with RIPK3 and enhances the interaction between RIPK3 and ZBP1, leading to ZBP1-mediated inflammasome activation and cell death (PubMed:32298652).

Biological Process

Apoptotic process Source: GO_Central
Cellular response to staurosporine Source: CAFA
Epithelial cell differentiation Source: UniProtKB
Execution phase of apoptosis Source: Reactome
Proteolysis Source: UniProtKB
Regulation of apoptotic process Source: Reactome

Cellular Location

Cytoplasm

PTM

Phosphorylated by NUAK1; phosphorylation inhibits self-activation.
Cleavages by caspase-8 and subsequently by caspase-3 generate the two active subunits.

Flores, J., Noël, A., Fillion, M. L., & LeBlanc, A. C. (2021). Therapeutic potential of Nlrp1 inflammasome, Caspase-1, or Caspase-6 against Alzheimer disease cognitive impairment. Cell Death & Differentiation, 1-13.

Zheng, M., Karki, R., Kancharana, B., Berns, H., Pruett-Miller, S. M., & Kanneganti, T. D. (2021). Caspase-6 promotes activation of the caspase-11-NLRP3 inflammasome during gram-negative bacterial infections. Journal of Biological Chemistry, 101379.

Noël, A., Foveau, B., & LeBlanc, A. C. (2021). Caspase-6-cleaved Tau fails to induce Tau hyperphosphorylation and aggregation, neurodegeneration, glial inflammation, and cognitive deficits. Cell death & disease, 12(3), 1-20.

Zhou, L., Flores, J., Noël, A., Beauchet, O., Sjöström, P. J., & LeBlanc, A. C. (2019). Methylene blue inhibits Caspase-6 activity, and reverses Caspase-6-induced cognitive impairment and neuroinflammation in aged mice. Acta neuropathologica communications, 7(1), 1-18.

Islam, M. I., Nagakannan, P., Ogungbola, O., Djordjevic, J., Albensi, B. C., & Eftekharpour, E. (2019). Thioredoxin system as a gatekeeper in caspase-6 activation and nuclear lamina integrity: Implications for Alzheimer's disease. Free Radical Biology and Medicine, 134, 567-580.

Tubeleviciute-Aydin, A., Zhou, L., Sharma, G., Soni, I. V., Savinov, S. N., Hardy, J. A., & LeBlanc, A. C. (2018). Rare human Caspase-6-R65W and Caspase-6-G66R variants identify a novel regulatory region of Caspase-6 activity. Scientific reports, 8(1), 1-14.

Girling, K. D., Demers, M. J., Laine, J., Zhang, S., Wang, Y. T., & Graham, R. K. (2018). Activation of caspase‐6 and cleavage of caspase‐6 substrates is an early event in NMDA receptor–mediated excitotoxicity. Journal of neuroscience research, 96(3), 391-406.

Offen, D., Angel, A., & Ben-Zur, T. (2018). Caspase-6 knock-out using CRISPR/Cas9 improves cognitive behavior in the 3xTg mouse model of Alzheimer's disease. Cytotherapy, 20(5), S94-S95.

Woo, V., Cheng, C., Duraikannu, A., Chandrasekhar, A., Purdy, K., Martinez, J. A., & Zochodne, D. W. (2018). Caspase-6 is a dispensable enabler of adult mammalian axonal degeneration. Neuroscience, 371, 242-253.

Skotte, N. H., Sanders, S. S., Singaraja, R. R., Ehrnhoefer, D. E., Vaid, K., Qiu, X., ... & Hayden, M. R. (2017). Palmitoylation of caspase-6 by HIP14 regulates its activation. Cell Death & Differentiation, 24(3), 433-444.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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