Human CASP6 ELISA Kit (V2LY-0626-LY4366)

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Tested Data
Datasheet
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Basic Information

Sensitivity
0.0078 ng/mL
Detection Range
0.015-3 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
33.3 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Caspase 6
Function
Cysteine protease that plays essential roles in programmed cell death, axonal degeneration, development and innate immunity (PubMed:8663580, PubMed:32298652).
During apoptosis, localizes in the nucleus and cleaves the nuclear structural protein NUMA1 and lamin A/LMNA thereby inducing nuclear shrinkage and fragmentation (PubMed:17401638, PubMed:8663580, PubMed:9463409).
Furthermore, cleaves many transcription factors such as NF-kappa-B and cAMP response element-binding protein/CREBBP (PubMed:10559921, PubMed:14657026).
Plays an essential role in axon degeneration during axon pruning which is the remodeling of axons during neurogenesis but not apoptosis (By similarity).
Regulates B-cell programs both during early development and after antigen stimulation (By similarity).
In addition, promotes the ZBP1-mediated activation of programmed cell death pathways including pyroptosis, apoptosis, and necroptosis (PANoptosis) and plays an essential role in defense against viruses (PubMed:32298652).
Mechanistically, interacts with RIPK3 and enhances the interaction between RIPK3 and ZBP1, leading to ZBP1-mediated inflammasome activation and cell death (PubMed:32298652).
Biological Process
Apoptotic process Source: GO_Central
Cellular response to staurosporine Source: CAFA
Epithelial cell differentiation Source: UniProtKB
Execution phase of apoptosis Source: Reactome
Proteolysis Source: UniProtKB
Regulation of apoptotic process Source: Reactome
Cellular Location
Cytoplasm
PTM
Phosphorylated by NUAK1; phosphorylation inhibits self-activation.
Cleavages by caspase-8 and subsequently by caspase-3 generate the two active subunits.

Flores, J., Noël, A., Fillion, M. L., & LeBlanc, A. C. (2021). Therapeutic potential of Nlrp1 inflammasome, Caspase-1, or Caspase-6 against Alzheimer disease cognitive impairment. Cell Death & Differentiation, 1-13.

Zheng, M., Karki, R., Kancharana, B., Berns, H., Pruett-Miller, S. M., & Kanneganti, T. D. (2021). Caspase-6 promotes activation of the caspase-11-NLRP3 inflammasome during gram-negative bacterial infections. Journal of Biological Chemistry, 101379.

Noël, A., Foveau, B., & LeBlanc, A. C. (2021). Caspase-6-cleaved Tau fails to induce Tau hyperphosphorylation and aggregation, neurodegeneration, glial inflammation, and cognitive deficits. Cell death & disease, 12(3), 1-20.

Zhou, L., Flores, J., Noël, A., Beauchet, O., Sjöström, P. J., & LeBlanc, A. C. (2019). Methylene blue inhibits Caspase-6 activity, and reverses Caspase-6-induced cognitive impairment and neuroinflammation in aged mice. Acta neuropathologica communications, 7(1), 1-18.

Islam, M. I., Nagakannan, P., Ogungbola, O., Djordjevic, J., Albensi, B. C., & Eftekharpour, E. (2019). Thioredoxin system as a gatekeeper in caspase-6 activation and nuclear lamina integrity: Implications for Alzheimer's disease. Free Radical Biology and Medicine, 134, 567-580.

Tubeleviciute-Aydin, A., Zhou, L., Sharma, G., Soni, I. V., Savinov, S. N., Hardy, J. A., & LeBlanc, A. C. (2018). Rare human Caspase-6-R65W and Caspase-6-G66R variants identify a novel regulatory region of Caspase-6 activity. Scientific reports, 8(1), 1-14.

Girling, K. D., Demers, M. J., Laine, J., Zhang, S., Wang, Y. T., & Graham, R. K. (2018). Activation of caspase‐6 and cleavage of caspase‐6 substrates is an early event in NMDA receptor–mediated excitotoxicity. Journal of neuroscience research, 96(3), 391-406.

Offen, D., Angel, A., & Ben-Zur, T. (2018). Caspase-6 knock-out using CRISPR/Cas9 improves cognitive behavior in the 3xTg mouse model of Alzheimer's disease. Cytotherapy, 20(5), S94-S95.

Woo, V., Cheng, C., Duraikannu, A., Chandrasekhar, A., Purdy, K., Martinez, J. A., & Zochodne, D. W. (2018). Caspase-6 is a dispensable enabler of adult mammalian axonal degeneration. Neuroscience, 371, 242-253.

Skotte, N. H., Sanders, S. S., Singaraja, R. R., Ehrnhoefer, D. E., Vaid, K., Qiu, X., ... & Hayden, M. R. (2017). Palmitoylation of caspase-6 by HIP14 regulates its activation. Cell Death & Differentiation, 24(3), 433-444.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Rat Casp6 ELISA Kit (CAT#: V2LY-0626-LY1671)

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Isotype control

For research use only. Not intended for any clinical use.

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