Rabbit Anti-CASP8AP2 Recombinant Antibody (0186) (CBMAB-0186-YC)

Name: Rabbit Anti-CASP8AP2 Recombinant Antibody (0186)
SKU: CBMAB-0186-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

0186

Application

Immunogen

Recombinant protein specific to residues near the amino terminus of human FLASH/CASP8AP2 protein.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

HEPES, pH 7.5, 100 µg/ml BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

CASP8AP2

Introduction

CASP8AP2 (caspase 8 associated protein 2) is a protein that in humans is encoded by the CASP8AP2 gene. CASP8AP2 is highly similar to FLASH, a mouse apoptotic protein identified by its interaction with the death-effector domain (DED) of caspase 8. This protein may be a component of the death-inducing signaling complex that includes Fas receptor, Fas-binding adapter FADD, and caspase 8, and plays a regulatory role in Fas-mediated apoptosis.

Entrez Gene ID

9994

UniProt ID

Q9UKL3

Alternative Names

CED-4; FLASH; FLJ11208; KIAA1315; RIP25

Function

Participates in TNF-alpha-induced blockade of glucocorticoid receptor (GR) transactivation at the nuclear receptor coactivator level, upstream and independently of NF-kappa-B. Suppresses both NCOA2- and NCOA3-induced enhancement of GR transactivation. Involved in TNF-alpha-induced activation of NF-kappa-B via a TRAF2-dependent pathway. Acts as a downstream mediator for CASP8-induced activation of NF-kappa-B. Required for the activation of CASP8 in FAS-mediated apoptosis. Required for histone gene transcription and progression through S phase.

Biological Process

Activation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
Apoptotic signaling pathway Source: ProtInc
Cell cycle Source: UniProtKB-KW
Cellular response to mechanical stimulus Source: UniProtKB
Extrinsic apoptotic signaling pathway via death domain receptors Source: UniProtKB
Fas signaling pathway Source: UniProtKB
Signal transduction Source: ProtInc

Cellular Location

Cytoplasm; Mitochondrion; Nucleus; PML body. Exported from the nucleus to the mitochondria upon FAS activation.

Abaandou, L., Sharma, A. K., & Shiloach, J. (2021). Knockout of the caspase 8‐associated protein 2 gene improves recombinant protein expression in HEK293 cells through up‐regulation of the cyclin‐dependent kinase inhibitor 2A gene. Biotechnology and Bioengineering, 118(1), 186-198.

Wu, T. Y., Leng, Q., & Tian, L. Q. (2021). The microRNA-210/Casp8ap2 Axis Alleviates Hypoxia-Induced Myocardial Injury by Regulating Apoptosis and Autophagy. Cytogenetic and Genome Research, 1-11.

Ibrahimova, A., Luebbering, N., Abdullah, S., Lane, A., Duell, A., Lake, K. E., ... & Myers, K. C. (2021, February). Dysregulation of Hypoxia Induced Factor 1 Alpha (HIF1α) and Caspase 8 Associated Protein 2 (CASP8AP2) in Patients with Graft Versus Host Disease (GVHD). In 2021 TCT| Transplantation & Cellular Therapy Meetings of ASTCT and CIBMTR. TCT Meetings.

Yu, X., Liu, F., Liu, Y., Bai, B., Yin, H., Wang, H., ... & Geng, Q. (2021). Omega-3 fatty acid protects cardiomyocytes against hypoxia-induced injury through targeting MiR-210-3p/CASP8AP2 axis. Molecular and Cellular Biochemistry, 1-9.

Li, K., Pan, J., Li, Q., Li, S., Cheng, Y., Chai, L., ... & Bai, Y. (2020, October). The MicroRNA-210/Casp8ap2 Pathway Alleviates Hypoxia-Induced Injury in Myocardial Cells by Regulating Apoptosis and Autophagy. In The Heart Surgery Forum (Vol. 23, No. 6, pp. E797-E802).

Liu, F. F., Wang, K. L., Deng, L. P., Liu, X., Wu, M. Y., Wang, T. Y., ... & Li, Z. G. (2018). Transcription factor E2F3a regulates CASP8AP2 transcription and enhances sensitivity to chemotherapeutic drugs in acute lymphoblastic leukemia. Cancer cell international, 18(1), 1-9.

Mei, Y., Li, Z., Zhang, Y., Zhang, W., Hu, H., Zhang, P., ... & Huang, D. (2017). Low miR‑210 and CASP8AP2 expression is associated with a poor outcome in pediatric acute lymphoblastic leukemia. Oncology letters, 14(6), 8072-8077.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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