Mouse Anti-CAST Recombinant Antibody (CBLNC-136) (CBMAB-1318-CN)

Name: Mouse Anti-CAST Recombinant Antibody (CBLNC-136)
SKU: CBMAB-1318-CN
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CAST Recombinant Antibody (CBLNC-136) (CBMAB-1318-CN) in WB

Western blot analysis of five selected GBM cell lines for their CAPN1, CAPN2, and calpastatin (CAST) expression. GAPDH was used as loading control. ...View More

Stillger, M. N., Chen, C. Y., Lai, Z. W., Li, M., Schäfer, A., Pagenstecher, A., ... & Schilling, O. (2023). Changes in calpain-2 expression during glioblastoma progression predisposes tumor cells to temozolomide resistance by minimizing DNA damage and p53-dependent apoptosis. Cancer cell international, 23(1), 49.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBLNC-136

Application

ELISA, IHC-P, IF, IP, WB

Immunogen

Amino acids 409-708 of Calpastatin of human origin.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:100-1:1,000
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Calpastatin

Introduction

Calpastatin is an endogenous calpain (calcium-dependent cysteine protease) inhibitor, which consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4). Calpastatin is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation.

Entrez Gene ID

Human	831
Cattle	281039
Rat	25403
Dog	479148
Pig	397135
Rabbit	100009098

UniProt ID

Human	P20810
Cattle	A0A4W2IP19
Rat	P27321
Dog	E2QXA6
Pig	P12675
Rabbit	P08855

Alternative Names

BS-17; PLACK

Function

Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue.

Biological Process

Inhibition of cysteine-type endopeptidase activity Source: CAFA
Negative regulation of type B pancreatic cell apoptotic process Source: BHF-UCL
Presynaptic active zone organization Source: ARUK-UCL

Cellular Location

Cytosol; Endoplasmic reticulum; Cytoplasm; Membrane

Involvement in disease

Peeling skin with leukonychia, acral punctate keratoses, cheilitis, and knuckle pads (PLACK): An autosomal recessive disease characterized by generalized, continuous shedding of the outer layers of the epidermis, leukonychia, acral punctate keratosis, cheilitis, knuckle pads with multiple hyperkeratotic micropapules involving the interphalangeal joints, and palmoplantar keratoderma.

PTM

The N-terminus is blocked.

Flores-Toro, J., Chun, S. K., Shin, J. K., Campbell, J., Lichtenberger, M., Chapman, W., ... & Kim, J. S. (2021). Critical Roles of Calpastatin in Ischemia/Reperfusion Injury in Aged Livers. Cells, 10(8), 1863.

Hu, D., Sun, X., Magpusao, A., Fedorov, Y., Thompson, M., Wang, B., ... & Qi, X. (2021). Small-molecule suppression of calpastatin degradation reduces neuropathology in models of Huntington’s disease. Nature communications, 12(1), 1-19.

Motter, M. M., Corva, P. M., & Soria, L. A. (2021). Expression of calpastatin isoforms in three skeletal muscles of Angus steers and their association with fiber type composition and proteolytic potential. Meat Science, 171, 108267.

Bozhilova-Sakova, M., Dimitrova, I., Tzonev, T. I., & Petrov, N. (2020). Genotype frequencies in calpastatin (CAST) and callipyge (CLPG) genes in Northeast Bulgarian Merino sheep breed using PCR-RFLP method. Bulgarian Journal of Agricultural Science, 26(2), 475-479.

Burchett, R., Brun, M., Monckton, E. A., Poon, H. Y., & Godbout, R. (2019). Effects of nuclear factor I phosphorylation on calpastatin (CAST) gene variant expression and subcellular distribution in malignant glioma cells. Journal of Biological Chemistry, 294(4), 1173-1188.

Wang, S., Huang, Y., Yan, Y., Zhou, H., Wang, M., Liao, L., ... & Xiong, K. (2019). Calpain2 but not calpain1 mediated by calpastatin following glutamate-induced regulated necrosis in rat retinal neurons. Annals of Anatomy-Anatomischer Anzeiger, 221, 57-67.

Jawasreh, K. I., & Ismail, Z. B. (2019). Effects of calpastatin gene polymorphism on hematology and selected serum biochemical parameters in Awassi lambs. Journal of advanced veterinary and animal research, 6(2), 193.

Zhang, J., Wu, D., Li, Y., Fan, Y., Chen, H., & Xu, J. (2018). Evaluating the association between calpastatin (CAST) gene and keratoconus in the Han Chinese population. Gene, 653, 10-13.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

CAST Matched Antibody Pair (185) (CAT#: APMAB-185LY)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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