Mouse Anti-CBFA2T3 Recombinant Antibody (CBFYC-0881) (CBMAB-C0936-FY)

Name: Mouse Anti-CBFA2T3 Recombinant Antibody (CBFYC-0881)
SKU: CBMAB-C0936-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYC-0881

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

Amino acids 625-653 at the C-terminus of ETO-2 of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:100-1:1,000
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

CBFA2/RUNX1 TRANSLOCATION PARTNER 3

Introduction

This gene encodes a member of the myeloid translocation gene family which interact with DNA-bound transcription factors and recruit a range of corepressors to facilitate transcriptional repression. The t(16;21)(q24;q22) translocation is one of the less common karyotypic abnormalities in acute myeloid leukemia. The translocation produces a chimeric gene made up of the 5'-region of the runt-related transcription factor 1 gene fused to the 3'-region of this gene. This gene is also a putative breast tumor suppressor. Alternative splicing results in transcript variants.

Entrez Gene ID

863

UniProt ID

O75081

Alternative Names

CBFA2/RUNX1 Translocation Partner 3; Core-Binding Factor, Runt Domain, Alpha Subunit 2; Translocated To, 3; Myeloid Translocation Gene On Chromosome 16 Protein; Zinc Finger MYND Domain-Containing Protein 4; Myeloid Translocation Gene 8 And 16b; MTG8-Related Protein 2; ZMYND4; MTG16

Function

Transcriptional corepressor which facilitates transcriptional repression via its association with DNA-binding transcription factors and recruitment of other corepressors and histone-modifying enzymes (PubMed:12559562, PubMed:15203199).
Can repress the expression of MMP7 in a ZBTB33-dependent manner (PubMed:23251453).
Reduces the protein levels and stability of the transcriptinal regulator HIF1A; interacts with EGLN1 and promotes the HIF1A prolyl hydroxylation-dependent ubiquitination and proteasomal degradation pathway (PubMed:25974097).
Contributes to inhibition of glycolysis and stimulation of mitochondrial respiration by down-regulating the expression of glycolytic genes including PFKFB3, PFKFB4, PDK1, PFKP, LDHA and HK1 which are direct targets of HIF1A (PubMed:23840896, PubMed:25974097).
Regulates the proliferation and the differentiation of erythroid progenitors by repressing the expression of TAL1 target genes (By similarity).
Plays a role in granulocyte differentiation (PubMed:15231665).
Isoform 2 functions as an A-kinase-anchoring protein (PubMed:11823486).

Biological Process

Granulocyte differentiation Source: MGI
Negative regulation of cell population proliferation Source: UniProtKB
Negative regulation of glycolytic process Source: UniProtKB
Negative regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
Regulation of aerobic respiration Source: UniProtKB
Response to hypoxia Source: UniProtKB
Transcription, DNA-templated Source: InterPro

Cellular Location

Isoform 1: Nucleolus. The RUNX1-CBFA2T3 fusion protein localizes to the nucleoplasm.
Isoform 2: Golgi apparatus membrane; Nucleoplasm

Involvement in disease

A chromosomal aberration involving CBFA2T3 is found in therapy-related myeloid malignancies. Translocation t(16;21)(q24;q22) that forms a RUNX1-CBFA2T3 fusion protein.

Steinauer, N., Guo, C., & Zhang, J. (2020). The transcriptional corepressor CBFA2T3 inhibits all-trans-retinoic acid–induced myeloid gene expression and differentiation in acute myeloid leukemia: EDITORS'PICK: CBFA2T3 inhibits ATRA-mediated myeloid maturation. Journal of Biological Chemistry, 295(27), 8887-8900.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-CBFA2T3 Recombinant Antibody (CBFYE-1338) (CAT#: CBMAB-E1947-FY)

Isotype control

For research use only. Not intended for any clinical use.

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