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Mouse Anti-CBLB Recombinant Antibody (CBFYC-0888) (CBMAB-C0943-FY)

This product is mouse antibody that recognizes CBLB. The antibody CBFYC-0888 can be used for immunoassay techniques such as: Dot, WB.
See all CBLB antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse
Clone
CBFYC-0888
Antibody Isotype
IgG2a
Application
Dot, WB

Basic Information

Immunogen
Recombinant Human Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence B
Specificity
Human, Mouse
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 1% BSA, pH 7.4
Preservative
0.05% Sodium azide
Concentration
0.1 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Cbl Proto-Oncogene B
Introduction
This gene encodes an E3 ubiquitin-protein ligase which promotes proteosome-mediated protein degradation by transferring ubiquitin from an E2 ubiquitin-conjugating enzyme to a substrate. The encoded protein is involved in the regulation of immune response by limiting T-cell receptor, B-cell receptor, and high affinity immunoglobulin epsilon receptor activation. Studies in mouse suggest that this gene is involved in antifungal host defense and that its inhibition leads to increased fungal killing. Manipulation of this gene may be beneficial in implementing immunotherapies for a variety of conditions, including cancer, autoimmune diseases, allergies, and infections.
Entrez Gene ID
Human868
Mouse208650
UniProt ID
HumanQ13191
MouseQ3TTA7
Alternative Names
Cbl Proto-Oncogene B; Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence B; Cbl Proto-Oncogene B, E3 Ubiquitin Protein Ligase; Casitas B-Lineage Lymphoma Proto-Oncogene B; RING-Type E3 Ubiquitin Transferase CBL-B; Signal Transduction Protein CBL-B; SH3-Binding Protein CBL-B; RING Finger Protein 56; RNF56
Function
E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Negatively regulates TCR (T-cell receptor), BCR (B-cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways. In naive T-cells, inhibits VAV1 activation upon TCR engagement and imposes a requirement for CD28 costimulation for proliferation and IL-2 production. Also acts by promoting PIK3R1/p85 ubiquitination, which impairs its recruitment to the TCR and subsequent activation. In activated T-cells, inhibits PLCG1 activation and calcium mobilization upon restimulation and promotes anergy. In B-cells, acts by ubiquitinating SYK and promoting its proteasomal degradation. Slightly promotes SRC ubiquitination. May be involved in EGFR ubiquitination and internalization. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3. In association with CBL, required for proper feedback inhibition of ciliary platelet-derived growth factor receptor-alpha (PDGFRA) signaling pathway via ubiquitination and internalization of PDGFRA (By similarity).
Biological Process
Immune response Source: Ensembl
Intracellular signal transduction Source: Ensembl
Negative regulation of alpha-beta T cell proliferation Source: Ensembl
Negative regulation of epidermal growth factor-activated receptor activity Source: GO_Central
Negative regulation of T cell receptor signaling pathway Source: Ensembl
NLS-bearing protein import into nucleus Source: ProtInc
Peptidyl-amino acid modification Source: Ensembl
Positive regulation of protein catabolic process Source: Ensembl
Positive regulation of protein ubiquitination Source: Ensembl
Positive regulation of T cell anergy Source: Ensembl
Regulation of GTPase activity Source: Ensembl
Regulation of platelet-derived growth factor receptor-alpha signaling pathway Source: UniProtKB
Regulation of protein binding Source: Ensembl
Signal transduction Source: GO_Central
T cell activation Source: Ensembl
T cell receptor signaling pathway Source: Ensembl
Cellular Location
Cytoplasm. Upon EGF stimulation, associates with endocytic vesicles.
PTM
Phosphorylated on tyrosine and serine residues upon TCR or BCR activation, and upon various types of cell stimulation.
Auto-ubiquitinated upon EGF-mediated cell activation or upon T-cell costimulation by CD28; which promotes proteasomal degradation.

Jiang, W., Wang, D., Wilson, B. A., Voeller, D., Bokesch, H. R., Smith, E. A., ... & Gustafson, K. R. (2021). Sinularamides A–G, Terpenoid-Derived Spermidine and Spermine Conjugates with Casitas B-Lineage Lymphoma Proto-Oncogene B (Cbl-b) Inhibitory Activities from a Sinularia sp. Soft Coral. Journal of Natural Products.

Chen, K., Wan, X., Zhao, L., Zhao, S., Peng, L., Yang, W., ... & Mo, Z. (2020). Cbl Proto-Oncogene B (CBLB) c. 197A> T Mutation Induces Mild Metabolic Dysfunction in Partial Type I Multiple Symmetric Lipomatosis (MSL). Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy, 13, 3535.

Liu, X., Teng, Y., Wu, X., Li, Z., Bao, B., Liu, Y., ... & Zhang, L. (2020). The E3 Ubiquitin Ligase Cbl-b Predicts Favorable Prognosis in Breast Cancer. Frontiers in Oncology, 10, 695.

BommaReddy, R. R., Patel, R., Smalley, T., & Acevedo-Duncan, M. (2020). Effects of atypical protein kinase C inhibitor (DNDA) on lung cancer proliferation and migration by PKC-ι/FAK ubiquitination through the Cbl-b pathway. OncoTargets and therapy, 13, 1661.

Tang, R., Langdon, W. Y., & Zhang, J. (2019). Regulation of immune responses by E3 ubiquitin ligase Cbl-b. Cellular immunology, 340, 103878.

Wesley, E. M., Xin, G., McAllister, D., Malarkannan, S., Newman, D. K., Dwinell, M. B., ... & Riese, M. J. (2018). Diacylglycerol kinase ζ (DGKζ) and Casitas b-lineage proto-oncogene b–deficient mice have similar functional outcomes in T cells but DGKζ-deficient mice have increased T cell activation and tumor clearance. Immunohorizons, 2(4), 107-118.

Riling, C., Sokirniy, I., Cunnion, B., Todd, E., Mattern, M., Wu, J., ... & Kumar, S. (2018). Abstract A206: Small-molecule Cbl-b inhibitors as novel intracellular checkpoint inhibitors for cancer immunotherapy.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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