Rabbit Anti-CBLB Recombinant Antibody (D3C12) (CBMAB-CP0150-LY)

Name: Rabbit Anti-CBLB Recombinant Antibody (D3C12)
SKU: CBMAB-CP0150-LY
Rating: 5 (1 reviews)

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Published Data

Rabbit Anti-CBLB Recombinant Antibody (D3C12) (CBMAB-CP0150-LY) in WB

cCbl- or Cbl-b-depleted (or control) SCC47 cells were serum-starved and treated with Sym004 (3 µg/ml or 30 µg/ml) or EGF for 6 h, then lysed. EGFR degradation was quantified as remaining EGFR (normalised to tubulin) relative to untreated cells. Error bars, SEM. *p<0.05. All immunoblots were cropped for clarity. ...View More

Jones, S., King, P. J., Antonescu, C. N., Sugiyama, M. G., Bhamra, A., Surinova, S., ... & Hochhauser, D. (2020). Targeting of EGFR by a combination of antibodies mediates unconventional EGFR trafficking and degradation. Scientific Reports, 10(1), 663.

Rabbit Anti-CBLB Recombinant Antibody (D3C12) (CBMAB-CP0150-LY) in Immunoblot

Immunoblots showing LynA, c-Cbl, and Cbl-b protein in CskAS, CskASc-CblKO, and CskASCbl-bKO BMDMs treated with 3-IB-PP1 for the indicated times. ...View More

Brian IV, B. F., Jolicoeur, A. S., Guerrero, C. R., Nunez, M. G., Sychev, Z. E., Hegre, S. A., ... & Freedman, T. S. (2019). Unique-region phosphorylation targets LynA for rapid degradation, tuning its expression and signaling in myeloid cells. Elife, 8, e46043.

Rabbit Anti-CBLB Recombinant Antibody (D3C12) (CBMAB-CP0150-LY) in IP

Abundance of ICN1, tubulin α, and Cbl-b in Jurkat cells expressing a control (Ctl.) and four independent (sh1 to sh4) CBLB shRNAs. Determinations were done by WB using either TCLs (ICN1, tubulin α) or immunoprecipitated Cbl-b. +Cbl-b, WT Jurkat cells ectopically expressing Cbl-bWT (n = 3). ...View More

Robles-Valero, J., Lorenzo-Martín, L. F., Menacho-Marquez, M., Fernandez-Pisonero, I., Abad, A., Camos, M., ... & Bustelo, X. R. (2017). A paradoxical tumor-suppressor role for the Rac1 exchange factor Vav1 in T cell acute lymphoblastic leukemia. Cancer Cell, 32(5), 608-623.

Rabbit Anti-CBLB Recombinant Antibody (D3C12) (CBMAB-CP0150-LY) in Immunoblot and IP

AG01523 fibroblasts were transfected with siRNAs targeting c-Cbl and Cbl-b and serum-starved overnight. Cells were then stimulated with 20 ng/ml PDGF-BB for the indicated periods of time, followed by lysis, PDGFRβ immunoprecipitation (Ip), SDS-PAGE and immunoblotting (Ib) for poly-ubiquitin (Ub, top panel). Total cell lysates (TCL) were also collected, and the efficiency of c-Cbl and Cbl-b depletion was analyzed (center and bottom panels). Ctrl, control. ...View More

Rorsman, C., Tsioumpekou, M., Heldin, C. H., & Lennartsson, J. (2016). The ubiquitin ligases c-Cbl and Cbl-b negatively regulate platelet-derived growth factor (PDGF) BB-induced chemotaxis by affecting PDGF receptor β (PDGFRβ) internalization and signaling. Journal of Biological Chemistry, 291(22), 11608-11618.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

D3C12

Application

Immunogen

Synthetic peptide corresponding to residues near the carboxy terminus of human Cbl-b protein.

Host Species

Rabbit

Specificity

Human, Mouse, Rat, Monkey

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

HEPES, pH 7.5, 100 µg/ml BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Cbl Proto-Oncogene B

Introduction

This gene encodes an E3 ubiquitin-protein ligase which promotes proteosome-mediated protein degradation by transferring ubiquitin from an E2 ubiquitin-conjugating enzyme to a substrate. The encoded protein is involved in the regulation of immune response by limiting T-cell receptor, B-cell receptor, and high affinity immunoglobulin epsilon receptor activation. Studies in mouse suggest that this gene is involved in antifungal host defense and that its inhibition leads to increased fungal killing. Manipulation of this gene may be beneficial in implementing immunotherapies for a variety of conditions, including cancer, autoimmune diseases, allergies, and infections. [provided by RefSeq, Sep 2017]

Entrez Gene ID

Human	868
Mouse	208650
Rat	171136
Monkey	703879
Cattle	525906

UniProt ID

Human	Q13191
Mouse	Q3TTA7
Rat	Q8K4S7
Monkey	A0A1D5Q8S8
Cattle	A0A3Q1LUB2

Alternative Names

Cbl Proto-Oncogene B; Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence B; Cbl Proto-Oncogene B; E3 Ubiquitin Protein Ligase; Casitas B-Lineage Lymphoma Proto-Oncogene B; RING-Type E3 Ubiquitin Transferase CBL-B; Signal Transduction Protein CBL-B; SH3-Binding Protein CBL-B; RING Finger Protein 56; RNF56;

Function

E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Negatively regulates TCR (T-cell receptor), BCR (B-cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways. In naive T-cells, inhibits VAV1 activation upon TCR engagement and imposes a requirement for CD28 costimulation for proliferation and IL-2 production. Also acts by promoting PIK3R1/p85 ubiquitination, which impairs its recruitment to the TCR and subsequent activation. In activated T-cells, inhibits PLCG1 activation and calcium mobilization upon restimulation and promotes anergy. In B-cells, acts by ubiquitinating SYK and promoting its proteasomal degradation. Slightly promotes SRC ubiquitination. May be involved in EGFR ubiquitination and internalization. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3. In association with CBL, required for proper feedback inhibition of ciliary platelet-derived growth factor receptor-alpha (PDGFRA) signaling pathway via ubiquitination and internalization of PDGFRA (By similarity).

Biological Process

Immune response Source: Ensembl
Intracellular signal transduction Source: Ensembl
Negative regulation of alpha-beta T cell proliferation Source: Ensembl
Negative regulation of epidermal growth factor-activated receptor activity Source: GO_Central
Negative regulation of T cell receptor signaling pathway Source: Ensembl
NLS-bearing protein import into nucleus Source: ProtInc
Peptidyl-amino acid modification Source: Ensembl
Positive regulation of protein catabolic process Source: Ensembl
Positive regulation of protein ubiquitination Source: Ensembl
Positive regulation of T cell anergy Source: Ensembl
Regulation of GTPase activity Source: Ensembl
Regulation of platelet-derived growth factor receptor-alpha signaling pathway Source: UniProtKB
Regulation of protein binding Source: Ensembl
Signal transduction Source: GO_Central
T cell activation Source: Ensembl
T cell receptor signaling pathway Source: Ensembl

Cellular Location

Cytoplasm. Upon EGF stimulation, associates with endocytic vesicles.

PTM

Phosphorylated on tyrosine and serine residues upon TCR or BCR activation, and upon various types of cell stimulation.
Auto-ubiquitinated upon EGF-mediated cell activation or upon T-cell costimulation by CD28; which promotes proteasomal degradation.

Jiang, W., Wang, D., Wilson, B. A., Voeller, D., Bokesch, H. R., Smith, E. A., ... & Gustafson, K. R. (2021). Sinularamides A–G, Terpenoid-Derived Spermidine and Spermine Conjugates with Casitas B-Lineage Lymphoma Proto-Oncogene B (Cbl-b) Inhibitory Activities from a Sinularia sp. Soft Coral. Journal of Natural Products.

Chen, K., Wan, X., Zhao, L., Zhao, S., Peng, L., Yang, W., ... & Mo, Z. (2020). Cbl Proto-Oncogene B (CBLB) c. 197A> T Mutation Induces Mild Metabolic Dysfunction in Partial Type I Multiple Symmetric Lipomatosis (MSL). Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy, 13, 3535.

Liu, X., Teng, Y., Wu, X., Li, Z., Bao, B., Liu, Y., ... & Zhang, L. (2020). The E3 Ubiquitin Ligase Cbl-b Predicts Favorable Prognosis in Breast Cancer. Frontiers in Oncology, 10, 695.

BommaReddy, R. R., Patel, R., Smalley, T., & Acevedo-Duncan, M. (2020). Effects of atypical protein kinase C inhibitor (DNDA) on lung cancer proliferation and migration by PKC-ι/FAK ubiquitination through the Cbl-b pathway. OncoTargets and therapy, 13, 1661.

Tang, R., Langdon, W. Y., & Zhang, J. (2019). Regulation of immune responses by E3 ubiquitin ligase Cbl-b. Cellular immunology, 340, 103878.

Wesley, E. M., Xin, G., McAllister, D., Malarkannan, S., Newman, D. K., Dwinell, M. B., ... & Riese, M. J. (2018). Diacylglycerol kinase ζ (DGKζ) and Casitas b-lineage proto-oncogene b–deficient mice have similar functional outcomes in T cells but DGKζ-deficient mice have increased T cell activation and tumor clearance. Immunohorizons, 2(4), 107-118.

Riling, C., Sokirniy, I., Cunnion, B., Todd, E., Mattern, M., Wu, J., ... & Kumar, S. (2018). Abstract A206: Small-molecule Cbl-b inhibitors as novel intracellular checkpoint inhibitors for cancer immunotherapy.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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