Rabbit Anti-CBX3 Monoclonal Antibody (CBFYH-2922) (CBMAB-H0859-FY)

Name: Rabbit Anti-CBX3 Monoclonal Antibody (CBFYH-2922)
SKU: CBMAB-H0859-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

CBFYH-2922

Application

WB, IHC-P, ELISA

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HP1 gamma/CBX3.

Host Species

Rabbit

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:500-1:1,000
IHC-P	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.3, 50% glycerol, 0.05% BSA

Preservative

0.05% sodium azide

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Chromobox 3

Introduction

At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. This protein binds histone H3 tails methylated at Lys-9 sites. This protein is also recruited to sites of ultraviolet-induced DNA damage and double-strand breaks. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene.

Entrez Gene ID

Human	11335
Mouse	12417

UniProt ID

Human	Q13185
Mouse	P23198

Alternative Names

Chromobox 3; Heterochromatin Protein 1 Homolog Gamma; Modifier 2 Protein; HECH; Chromobox Homolog 3 (HP1 Gamma Homolog, Drosophila); Chromobox Homolog 3 (Drosophila HP1 Gamma); Heterochromatin Protein HP1 Gamma; HP1 Gamma Homolog (Drosophila)

Function

Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation, mediates the recruitment of the methyltransferases SUV39H1 and/or SUV39H2 by the PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1. Mediates the recruitment of NIPBL to sites of DNA damage at double-strand breaks (DSBs) (PubMed:28167679).

Biological Process

Cellular response to dexamethasone stimulus Source: Ensembl
Cellular response to DNA damage stimulus Source: UniProtKB
Chromatin remodeling Source: UniProtKB
Negative regulation of G0 to G1 transition Source: Reactome
Negative regulation of transcription, DNA-templated Source: UniProtKB
Rhythmic process Source: UniProtKB-KW

Cellular Location

Nucleus. Associates with euchromatin and is largely excluded from constitutive heterochromatin. May be associated with microtubules and mitotic poles during mitosis (Potential).

PTM

Phosphorylated by PIM1. Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.

Huang, Y., Lin, Y., Song, X., & Wu, D. (2021). LINC00857 contributes to proliferation and lymphomagenesis by regulating miR-370-3p/CBX3 axis in diffuse large B-cell lymphoma. Carcinogenesis, 42(5), 733-741.

Niu, H., Chen, P., Fan, L., & Sun, B. (2021). Comprehensive Pan-Cancer Analysis on CBX3 as a Prognostic and Immunological Biomarker.

He, Z., Chen, J., Chen, X., Wang, H., Tang, L., & Han, C. (2021). microRNA‐377 acts as a suppressor in esophageal squamous cell carcinoma through CBX3‐dependent P53/P21 pathway. Journal of cellular physiology, 236(1), 107-120.

Lin, H., Lian, J., Xia, L., Guan, G., & You, J. (2020). CBX3 promotes gastric cancer progression and affects factors related to immunotherapeutic responses. Cancer management and research, 12, 10113.

Bagheri, L., Thaler, R., Badreldin, A., Paradise, C. R., Khani, F., Deyle, D., ... & van Wijnen, A. (2020). Epigenetic Control of Osteoblast Differentiation by Chromobox 3 (Cbx3) Protein 3. The FASEB Journal, 34(S1), 1-1.

Lin, H., Zhao, X., Xia, L., Lian, J., & You, J. (2020). Clinicopathological and Prognostic Significance of CBX3 Expression in Human Cancer: a Systematic Review and Meta-analysis. Disease markers, 2020.

Liu, J., Zhan, Y., Wang, J., Wang, J., Guo, J., & Kong, D. (2020). lncRNA-SNHG17 promotes colon adenocarcinoma progression and serves as a sponge for miR-375 to regulate CBX3 expression. American Journal of Translational Research, 12(9), 5283.

Ma, C., Nie, X. G., Wang, Y. L., Liu, X. H., Liang, X., Zhou, Q. L., & Wu, D. P. (2019). CBX3 predicts an unfavorable prognosis and promotes tumorigenesis in osteosarcoma. Molecular medicine reports, 19(5), 4205-4212.

Zhang, H., Chen, W., Fu, X., Su, X., & Yang, A. (2018). CBX3 promotes tumor proliferation by regulating G1/S phase via p21 downregulation and associates with poor prognosis in tongue squamous cell carcinoma. Gene, 654, 49-56.

Meng, L., Wang, F., Sun, S., Zheng, Y., Ding, Z., Sun, Y., ... & Xu, M. (2017). MicroRNA-30b targets CBX3 and regulates cell proliferation, apoptosis, and migration in esophageal squamous cell carcinoma via the JAK2/STAT3 signaling pathway. International journal of clinical and experimental pathology, 10(12), 11828.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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