Rabbit Anti-CBX4 Recombinant Antibody (E6L7X) (CBMAB-CP0154-LY)

Name: Rabbit Anti-CBX4 Recombinant Antibody (E6L7X)
SKU: CBMAB-CP0154-LY
Rating: 5 (1 reviews)

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Published Data

Rabbit Anti-CBX4 Recombinant Antibody (E6L7X) (CBMAB-CP0154-LY) in IF

Tubulin was used as a loading control. Representative images (left panels) of BMI1 co-staining (green foci) with CBX4 (red foci) for cells harboring CAP bodies. ...View More

Azzoni, V., Wicinski, J., Macario, M., Castagné, M., Finetti, P., Ambrosova, K., ... & Ginestier, C. (2022). BMI1 nuclear location is critical for RAD51-dependent response to replication stress and drives chemoresistance in breast cancer stem cells. Cell Death & Disease, 13(2), 96.

Rabbit Anti-CBX4 Recombinant Antibody (E6L7X) (CBMAB-CP0154-LY) in IF

Confocal images of untreated and heat shocked K562 cells that were fixed and stained with anti-CBX4 and anti-NPM1. Scale bar represents 10 μm. ...View More

Azkanaz, M., Rodríguez López, A., de Boer, B., Huiting, W., Angrand, P. O., Vellenga, E., ... & van den Boom, V. (2019). Protein quality control in the nucleolus safeguards recovery of epigenetic regulators after heat shock. Elife, 8, e45205.

Rabbit Anti-CBX4 Recombinant Antibody (E6L7X) (CBMAB-CP0154-LY) in WB

CBX4 gene expression (n = 6) and protein expression (representative Western blot) in the CBX4 WT and control lentiviral construct (Ctrl) transduced OA chondrocytes. Upon longer exposure of the Western blot, CBX4 protein expression in control transduced primary chondrocytes was comparable to levels of expression we observe in senescent WI-38 (passage 55) cells (data not shown). CBX4: 75 kDa, β-actin: 42 kDa. ...View More

Chen, Y. H., Zhang, X., Attarian, D., & Kraus, V. B. (2023). Synergistic roles of CBX4 chromo and SIM domains in regulating senescence of primary human osteoarthritic chondrocytes. Arthritis Research & Therapy, 25(1), 197.

Rabbit Anti-CBX4 Recombinant Antibody (E6L7X) (CBMAB-CP0154-LY) in WB

CBX4 protein expression decreased significantly with serial passage (rrm = 0.877, p < 0.001); in contrast, CBX4 gene expression increased with serial passage (rrm = 0.555, p < 0.001). ...View More

Chen, Y. H., Zhang, X., Ko, K. Y., Hsueh, M. F., & Kraus, V. B. (2022). CBX4 regulates replicative senescence of WI-38 fibroblasts. Oxidative medicine and cellular longevity, 2022(1), 5503575.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

E6L7X

Application

WB, IP, IF (ICC)

Immunogen

Synthetic peptide corresponding to residues surrounding Pro166 of human CBX4 protein.

Host Species

Rabbit

Specificity

Human, Mouse, Monkey

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IF(ICC)	1:100
WB	1:1,000
IP	1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

HEPES, pH 7.5, 100 µg/ml BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Chromobox 4

Introduction

CBX4 (Chromobox 4) is a Protein Coding gene. Among its related pathways are SUMOylation and Metabolism of proteins. Gene Ontology (GO) annotations related to this gene include chromatin binding and ligase activity. An important paralog of this gene is CBX2.

Entrez Gene ID

Human	8535
Mouse	12418
Monkey	103243685

UniProt ID

Human	O00257
Mouse	O55187
Monkey	A0A2K5URM1

Alternative Names

Chromobox 4; Chromobox Protein Homolog 4; NS5ATP1-Binding Protein 16; Polycomb 2 Homolog; PC2; Chromobox Homolog 4 (Pc Class Homolog; Drosophila); Chromobox Homolog 4 (Drosophila Pc Class); Pc Class 2 Homolog (Drosophila);

Function

E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I (PubMed:12679040).
Involved in the sumoylation of HNRNPK, a p53/TP53 transcriptional coactivator, hence indirectly regulates p53/TP53 transcriptional activation resulting in p21/CDKN1A expression. Monosumoylates ZNF131 (PubMed:22825850).
Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development (PubMed:12167701, PubMed:19636380, PubMed:21282530).
PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed:12167701, PubMed:19636380, PubMed:21282530).
Binds to histone H3 trimethylated at 'Lys-9' (H3K9me3) (By similarity).
Plays a role in the lineage differentiation of the germ layers in embryonic development (By similarity).

Biological Process

Chromatin organization Source: UniProtKB-KW
Negative regulation of apoptotic process Source: ProtInc
Negative regulation of transcription, DNA-templated Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: UniProtKB

Cellular Location

Nucleus; Nucleus speckle. Localization to nuclear polycomb bodies is required for ZNF131 sumoylation (PubMed:22467880). Localized in distinct foci on chromatin (PubMed:18927235).

PTM

Phosphorylated on Thr-497 by HIPK2 upon DNA damage. This phosphorylation stimulates E3 SUMO-protein ligase activity and promotes sumoylation on Lys-494, as well as sumoylation of other target proteins, such as HNRNPK.

Wang, Z., Fang, Z., Chen, G., Liu, B., Xu, J., Li, F., ... & Ji, H. (2021). Chromobox 4 facilitates tumorigenesis of lung adenocarcinoma through the Wnt/β-catenin pathway. Neoplasia, 23(2), 222-233.

Yang, Z., OuYang, X., Zheng, L., Dai, L., & Luo, W. (2021). Long intergenic noncoding RNA00265 promotes proliferation of gastric cancer via the microRNA-144-3p/Chromobox 4 axis. Bioengineered, 12(1), 1012-1025.

Wen, L. J., Wang, Y. S., & Tan, P. Y. (2021). miR‑515‑5p inhibits the proliferation, migration and invasion of human breast cancer cells by targeting CBX4. Experimental and Therapeutic Medicine, 22(5), 1-9.

Zhao, J., Yang, T., & Li, L. (2020). LncRNA FOXP4-AS1 is involved in cervical cancer progression via regulating miR-136-5p/CBX4 axis. OncoTargets and therapy, 13, 2347.

Wang, X., Qin, G., Liang, X., Wang, W., Wang, Z., Liao, D., ... & Kang, T. (2020). Targeting the CK1α/CBX4 axis for metastasis in osteosarcoma. Nature communications, 11(1), 1-14.

Hu, C., Zhang, Q., Tang, Q., Zhou, H., Liu, W., Huang, J., ... & Zhang, R. (2020). CBX4 promotes the proliferation and metastasis via regulating BMI‐1 in lung cancer. Journal of cellular and molecular medicine, 24(1), 618-631.

Huang, F. J., Dang, J. Q., Zhang, S., & Cheng, Z. Y. (2020). Circular RNA hsa_circ_0008039 promotes proliferation, migration and invasion of breast cancer cells through upregulating CBX4 via sponging miR-515-5p. Eur Rev Med Pharmacol Sci, 24(4), 1887-1898.

Zeng, J. S., Zhang, Z. D., Pei, L., Bai, Z. Z., Yang, Y., Yang, H., & Tian, Q. H. (2018). CBX4 exhibits oncogenic activities in breast cancer via Notch1 signaling. The international journal of biochemistry & cell biology, 95, 1-8.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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