Mouse Anti-CBX5 Recombinant Antibody (CBT50) (V2LY-0625-LY3839)

Name: Mouse Anti-CBX5 Recombinant Antibody (CBT50)
SKU: V2LY-0625-LY3839
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-CBX5 Recombinant Antibody (CBT50) (V2LY-0625-LY3839) in IF

Mouse Anti-CBX5 Recombinant Antibody (CBT50) (V2LY-0625-LY3839) in IHC

Mouse Anti-CBX5 Recombinant Antibody (CBT50) (V2LY-0625-LY3839) in WB

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBT50

Application

IF, ICC, WB, IHC-P

Immunogen

Recombinant Protein of HP-1α

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
IF	1:50-1:200
IHC-P	1:50-1:300
WB	1:500-1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, Glycerol, BSA

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Chromobox 5

Entrez Gene ID

23468

UniProt ID

P45973

Alternative Names

Chromobox 5; Chromobox Homolog 5 (HP1 Alpha Homolog, Drosophila); Heterochromatin Protein 1 Homolog Alpha; Antigen P25; HP1A; Chromobox Homolog 5 (Drosophila HP1 Alpha); Heterochromatin Protein 1-Alpha; HP1 Alpha Homolog (Drosophila); Epididymis Luminal Protein 25;

Function

Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.

Biological Process

Blood coagulation Source: Reactome
Cellular response to DNA damage stimulus Source: UniProtKB
Negative regulation of G0 to G1 transition Source: Reactome
Negative regulation of transcription, DNA-templated Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: UniProtKB
Viral process Source: UniProtKB-KW

Cellular Location

Nucleus; Chromosome; Centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. The phosphorylated form at Ser-1083 is preferentially associated with unsynapsed chromosomal regions (By similarity).

PTM

Phosphorylation of HP1 and LBR may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle (By similarity). Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.
Ubiquitinated.

Yu, L., Zhang, W., Wang, P., Zhang, Q., Cong, A., Yang, X., & Sang, K. (2021). LncRNA SNHG11 aggravates cell proliferation and migration in triple‑negative breast cancer via sponging miR‑2355‑5p and targeting CBX5. Experimental and Therapeutic Medicine, 22(2), 1-11.

Lee, J., Pham, T. X., Guan, J., Caporarello, N., Meridew, J. A., Choi, K. M., ... & Ligresti, G. (2021). The Epigenetic Repressor CBX5 Drives Fibroblast Metabolic Reprograming and Lung Fibrogenesis. In C9. C009 MODULATORS OF LUNG INJURY AND REPAIR (pp. A1144-A1144). American Thoracic Society.

Wu, C., & Zhang, J. (2020). Long non-conding RNA LOXL1-AS1 sponges miR-589-5p to up-regulate CBX5 expression in renal cell carcinoma. Bioscience reports, 40(11), BSR20200212.

Ligresti, G., Caporarello, N., Meridew, J. A., Jones, D. L., Tan, Q., Choi, K. M., ... & Tschumperlin, D. J. (2019). CBX5/G9a/H3K9me-mediated gene repression is essential to fibroblast activation during lung fibrosis. JCI insight, 4(12).

Wang, H., Zhang, X. M., Tomiyoshi, G. O., Nakamura, R., Shinmen, N., Kuroda, H., ... & Hiwasa, T. (2018). Association of serum levels of antibodies against MMP1, CBX1, and CBX5 with transient ischemic attack and cerebral infarction. Oncotarget, 9(5), 5600.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

CBX5 Matched Antibody Pair (193) (CAT#: APMAB-193LY)

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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