Mouse Anti-CCAR1 Recombinant Antibody (CBXC-1772) (CBMAB-C5516-CQ)

Name: Mouse Anti-CCAR1 Recombinant Antibody (CBXC-1772)
SKU: CBMAB-C5516-CQ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBXC-1772

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 851-1150 mapping at the C-terminus of CARP-1 of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:100-1:1,000
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Cell Division Cycle And Apoptosis Regulator 1

Introduction

CCAR1 (Cell Division Cycle And Apoptosis Regulator 1) is a Protein Coding gene. Diseases associated with CCAR1 include Dissociative Amnesia and Multiple Personality Disorder. Among its related pathways are Gene Expression and mRNA Splicing - Major Pathway. Gene Ontology (GO) annotations related to this gene include nucleic acid binding and ligand-dependent nuclear receptor transcription coactivator activity. An important paralog of this gene is CCAR2.

Entrez Gene ID

55749

UniProt ID

Q8IX12

Alternative Names

Cell Division Cycle And Apoptosis Regulator 1; Cell Cycle And Apoptosis Regulatory Protein 1; Death Inducer With SAP Domain; CARP-1; CARP1; DIS;

Function

Associates with components of the Mediator and p160 coactivator complexes that play a role as intermediaries transducing regulatory signals from upstream transcriptional activator proteins to basal transcription machinery at the core promoter. Recruited to endogenous nuclear receptor target genes in response to the appropriate hormone. Also functions as a p53 coactivator. May thus play an important role in transcriptional regulation (By similarity).
May be involved in apoptosis signaling in the presence of the reinoid CD437. Apoptosis induction involves sequestration of 14-3-3 protein(s) and mediated altered expression of multiple cell cycle regulatory genes including MYC, CCNB1 and CDKN1A. Plays a role in cell cycle progression and/or cell proliferation (PubMed:12816952).
In association with CALCOCO1 enhances GATA1- and MED1-mediated transcriptional activation from the gamma-globin promoter during erythroid differentiation of K562 erythroleukemia cells (PubMed:24245781).
Can act as a both a coactivator and corepressor of AR-mediated transcription. Contributes to chromatin looping and AR transcription complex assembly by stabilizing AR-GATA2 association on chromatin and facilitating MED1 and RNA polymerase II recruitment to AR-binding sites. May play an important role in the growth and tumorigenesis of prostate cancer cells (PubMed:23887938).

Biological Process

Apoptotic process Source: UniProtKB-KW
Cell cycle Source: UniProtKB-KW
mRNA splicing, via spliceosome Source: Reactome
Positive regulation of apoptotic process Source: MGI
Positive regulation of cell migration Source: UniProtKB
Positive regulation of cell population proliferation Source: UniProtKB
Regulation of transcription, DNA-templated Source: GO_Central

Cellular Location

Perinuclear region

Westerheide, S. D., Lugano, D. I., Deonarine, A., & Park, M. A. (2021). CCAR-1 has a novel role in regulating the Caenorhabditis elegans germline.

Mondal, R., & Das, P. (2020). Novel aspects of Cell Division Cycle and Apoptosis Regulator 1 (CCAR1) protein in Morus notabilis: an in silico approach. Plant Signaling & Behavior, 15(10), 1795396.

Wang, F., Fan, M., Cai, Y., Zhou, X., Tai, S., Yu, Y., ... & Jin, X. (2020). Circular RNA circRIMS1 acts as a sponge of miR-433-3p to promote bladder cancer progression by regulating CCAR1 expression. Molecular Therapy-Nucleic Acids, 22, 815-831.

Li, D., Wang, L., Feng, J., Shen, Y. W., Liu, L. N., & Wang, Y. (2020). RP11‑284F21. 9 promotes lung carcinoma proliferation and invasion via the regulation of miR‑627‑3p/CCAR1. Oncology reports, 44(4), 1638-1648.

Johnson, G. S., Rajendran, P., & Dashwood, R. H. (2020). CCAR1 and CCAR2 as gene chameleons with antagonistic duality: Preclinical, human translational, and mechanistic basis. Cancer Science, 111(10), 3416.

Ge, Y., Tu, W., Li, J., Chen, X., Chen, Y., Xu, Y., ... & Liu, Y. (2020). MiR-122-5p increases radiosensitivity and aggravates radiation-induced rectal injury through CCAR1. Toxicology and applied pharmacology, 399, 115054.

Kanno, Y., Zhao, S., Yamashita, N., Saito, N., Ujiie, A., Iijima, R., ... & Inouye, Y. (2019). Cell cycle and apoptosis regulator 1, CCAR1, regulates enhancer-dependent nuclear receptor CAR transactivation. Molecular pharmacology, 95(1), 120-126.

Zhu, Y. J., Zheng, B., Luo, G. J., Ma, X. K., Lu, X. Y., Lin, X. M., ... & Chen, L. (2019). Circular RNAs negatively regulate cancer stem cells by physically binding FMRP against CCAR1 complex in hepatocellular carcinoma. Theranostics, 9(12), 3526.

Zhu, Y. J., Zheng, B., Ma, X. K., Lu, X. Y., Lin, X. M., Luo, G. J., ... & Chen, L. (2018). Circular RNAs regulate cancer stem cells by FMRP against CCAR1 complex in hepatocellular carcinoma. bioRxiv, 396069.

Chang, T. S., Wei, K. L., Lu, C. K., Chen, Y. H., Cheng, Y. T., Tung, S. Y., ... & Chiang, M. K. (2017). Inhibition of CCAR1, a coactivator of β-catenin, suppresses the proliferation and migration of gastric cancer cells. International journal of molecular sciences, 18(2), 460.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

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