Mouse Anti-CCL18 Recombinant Antibody (CBXC-1272) (CBMAB-C1539-CQ)

Name: Mouse Anti-CCL18 Recombinant Antibody (CBXC-1272)
SKU: CBMAB-C1539-CQ
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CCL18 Recombinant Antibody (CBXC-1272) (CBMAB-C1539-CQ) in IHC

Representative microphotography showing the same region of tumor (obj. magnification a, b × 20, scale bar 200 µm; a, b × 40, scale bar 100 µm). (a,b) Immunoexpression of CD68 (marker of macrophages), red arrows indicate this cells. (c,d) Immunoexpression of CCL18, green arrows show that macrophages produce and liberate CCL18 into ECM of glioblastoma. ...View More

Grochans, S., Korbecki, J., Simińska, D., Żwierełło, W., Rzeszotek, S., Kolasa, A., ... & Baranowska-Bosiacka, I. (2022). CCL18 expression is higher in a glioblastoma multiforme tumor than in the peritumoral area and causes the migration of tumor cells sensitized by hypoxia. International Journal of Molecular Sciences, 23(15), 8536.

Mouse Anti-CCL18 Recombinant Antibody (CBXC-1272) (CBMAB-C1539-CQ) in IHC

Representative micrographs showing the migration assay. U-87 MG cells cultured in CoCl2 hypoxia mimic agent (lower panel) showed a stronger response to CCL18 compared to cells grown under normal conditions (upper panel). CCL18 concentrations were 10 ng/mL, 20 ng/mL and 50 ng/mL. Control medium contained PBS. Pictures were captured with a Leica DM5000B, Wetzlar, Germany, objective magnification ×4, scale bar 500 µm. ...View More

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBXC-1272

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

Amino acids 61-89 at the C-terminus of MIP-4 of human origin.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:100-1:1,000
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated)

Introduction

CCL18 (C-C Motif Chemokine Ligand 18) is a Protein Coding gene. Diseases associated with CCL18 include Gaucher's Disease and Pulmonary Fibrosis. Among its related pathways are PEDF Induced Signaling and Akt Signaling. Gene Ontology (GO) annotations related to this gene include chemokine activity. An important paralog of this gene is CCL3.

Entrez Gene ID

6362

UniProt ID

P55774

Alternative Names

C-C Motif Chemokine Ligand 18; Small Inducible Cytokine Subfamily A (Cys-Cys), Member 18, Pulmonary And Activation-Regulated; Chemokine (C-C Motif) Ligand 18 (Pulmonary And Activation-Regulated); Alternative Macrophage Activation-Associated CC Chemokine 1; Pulmonary And Activation-Regulated Chemokine; Macrophage Inflammatory Protein 4; Dendritic Cell Chemokine 1; CC Chemokine PARC; SCYA18; AMAC-1; DC-CK1; AMAC1; DCCK1;

Function

Chemotactic factor that attracts lymphocytes but not monocytes or granulocytes. May be involved in B-cell migration into B-cell follicles in lymph nodes. Attracts naive T-lymphocytes toward dendritic cells and activated macrophages in lymph nodes, has chemotactic activity for naive T-cells, CD4+ and CD8+ T-cells and thus may play a role in both humoral and cell-mediated immunity responses.

Biological Process

Antimicrobial humoral immune response mediated by antimicrobial peptide Source: UniProtKB
Cell-cell signaling Source: ProtInc
Cell communication Source: ProtInc
Cellular response to interferon-gamma Source: GO_Central
Cellular response to interleukin-1 Source: GO_Central
Cellular response to tumor necrosis factor Source: GO_Central
Chemokine-mediated signaling pathway Source: GO_Central
Chemotaxis Source: ProtInc
G protein-coupled receptor signaling pathway Source: GO_Central
Immune response Source: ProtInc
Inflammatory response Source: GO_Central
Killing of cells of other organism Source: UniProtKB
Lymphocyte chemotaxis Source: GO_Central
Monocyte chemotaxis Source: GO_Central
Neutrophil chemotaxis Source: GO_Central
Positive regulation of ERK1 and ERK2 cascade Source: GO_Central
Positive regulation of GTPase activity Source: GO_Central
Signal transduction Source: ProtInc

Cellular Location

Secreted

PTM

The Cys-30/Cys-54 disulfide bond is required for activity.

Aravind, A., Palollathil, A., Rex, D. A. B., Kumar, K. M. K., Vijayakumar, M., Shetty, R., ... & Raju, R. (2021). A multi-cellular molecular signaling and functional network map of C–C motif chemokine ligand 18 (CCL18): a chemokine with immunosuppressive and pro-tumor functions. Journal of Cell Communication and Signaling, 1-8.

Sun, Z., Du, C., Xu, P., & Miao, C. (2019). Surgical trauma‐induced CCL18 promotes recruitment of regulatory T cells and colon cancer progression. Journal of cellular physiology, 234(4), 4608-4616.

Ma, L., Wang, H., Li, Z., Geng, X., & Li, M. (2019). Chemokine (CC motif) ligand 18 is highly expressed in glioma tissues and promotes invasion of glioblastoma cells. Journal of cancer research and therapeutics, 15(2), 358.

Huang, H., Li, J., Hu, W. J., Chen, C., Luo, H. Q., Tang, X. D., ... & Li, X. Y. (2019). The serum level of CC chemokine ligand 18 correlates with the prognosis of non-small cell lung cancer. The International journal of biological markers, 34(2), 156-162.

Song, H., Tao, Y., Ni, N., Zhou, X., Xiong, J., Zeng, X., ... & Sun, J. (2018). miR-128 targets the CC chemokine ligand 18 gene (CCL18) in cutaneous malignant melanoma progression. Journal of dermatological science, 91(3), 317-324.

Orekhov, A. N., Nikiforov, N. G., Elizova, N. V., Korobov, G. A., Aladinskaya, A. V., Sobenin, I. A., & Bobryshev, Y. V. (2018). Tumor necrosis factor-α and CC motif chemokine ligand 18 associate with atherosclerotic lipid accumulation in situ and in vitro. Current pharmaceutical design, 24(24), 2883-2889.

Huang, H., Li, J., Hu, W. J., Chen, M. H., Chen, S. S., Chen, C., ... & Li, X. Y. (2018). Positive expression of chemokine (CC Motif) ligand 18 and prognosis in cancer: A meta-analysis. Journal of BU ON.: official journal of the Balkan Union of Oncology, 23(4), 1185-1194.

Yuan, L., Wan, J., Huang, C., Liang, J., Liu, M., Yue, C., & Li, L. (2018). Evaluation of serum CCL18 as a potential biomarker for ovarian cancer. Cancer Biomarkers, 21(1), 97-104.

Bo, S., Donghao, S., Guangqi, K., & Ye, T. (2018). CC Chemokine Ligand 18 Promotes Cell Proliferation and Metastasis of Urothelial Carcinoma via Activating PI3K/mTOR Signaling in Patient with Renal Transplantation. Urologia internationalis, 101(4), 450-458.

Wang, H., Liang, X., Li, M., Tao, X., Tai, S., Fan, Z., ... & Xia, J. (2017). Chemokine (CC motif) ligand 18 upregulates Slug expression to promote stem‐cell like features by activating the mammalian target of rapamycin pathway in oral squamous cell carcinoma. Cancer science, 108(8), 1584-1593.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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