Sign in or Register   Sign in or Register
  |  

Mouse Anti-CCL20 (AA 27-96) Recombinant Antibody (CBFYM-0922) (CBMAB-M1070-FY)

This product is mouse antibody that recognizes CCL20. The antibody CBFYM-0922 can be used for immunoassay techniques such as: ELISA, FC, Neut, WB.
See all CCL20 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYM-0922
Antibody Isotype
IgG1
Application
ELISA, FC, Neut, WB

Basic Information

Immunogen
Recombinant human Macrophage Inflammatory Protein 3 alpha, Ala27-Met96 (Asn95Asp), expressed in E. coli (P78556)
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Buffer
PBS, pH 7.2, 5% trehalose
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 27-96

Target

Full Name
C-C Motif Chemokine Ligand 20
Introduction
CCL20 is a Protein Coding gene. Diseases associated with CCL20 include Rheumatoid Arthritis and Psoriasis. Among its related pathways are PEDF Induced Signaling and Bacterial infections in CF airways. Gene Ontology annotations related to this gene include cytokine activity and chemokine activity.
Entrez Gene ID
6364
UniProt ID
P78556
Alternative Names
C-C Motif Chemokine Ligand 20; Small Inducible Cytokine Subfamily A (Cys-Cys), Member 20; Liver And Activation-Regulated Chemokine; Macrophage Inflammatory Protein 3 Alpha; Chemokine (C-C Motif) Ligand 20; Small-Inducible Cytokine A20; CC Chemokine LARC; MIP-3-Alpha; SCYA20; MIP3A
Function
Acts as a ligand for C-C chemokine receptor CCR6. Signals through binding and activation of CCR6 and induces a strong chemotactic response and mobilization of intracellular calcium ions (PubMed:11352563, PubMed:11035086, PubMed:20068036).
The ligand-receptor pair CCL20-CCR6 is responsible for the chemotaxis of dendritic cells (DC), effector/memory T-cells and B-cells and plays an important role at skin and mucosal surfaces under homeostatic and inflammatory conditions, as well as in pathology, including cancer and various autoimmune diseases (PubMed:21376174).
CCL20 acts as a chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes (PubMed:9038201, PubMed:11352563).
Involved in the recruitment of both the proinflammatory IL17 producing helper T-cells (Th17) and the regulatory T-cells (Treg) to sites of inflammation. Required for optimal migration of thymic natural regulatory T cells (nTregs) and DN1 early thymocyte progenitor cells (By similarity).
C-terminal processed forms have been shown to be equally chemotactically active for leukocytes (PubMed:11035086).
Positively regulates sperm motility and chemotaxis via its binding to CCR6 which triggers Ca2+ mobilization in the sperm which is important for its motility (PubMed:23765988, PubMed:25122636).
Inhibits proliferation of myeloid progenitors in colony formation assays (PubMed:9129037).
May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells (By similarity).
Biological Process
Antimicrobial humoral immune response mediated by antimicrobial peptide Source: UniProtKB
Calcium-mediated signaling using intracellular calcium source Source: UniProtKB
Cell-cell signaling Source: ProtInc
Cell chemotaxis Source: UniProtKB
Cellular response to interferon-gamma Source: GO_Central
Cellular response to interleukin-1 Source: GO_Central
Cellular response to tumor necrosis factor Source: GO_Central
Chemokine-mediated signaling pathway Source: GO_Central
Chemotaxis Source: UniProtKB
Cytokine-mediated signaling pathway Source: Reactome
Defense response to bacterium Source: UniProtKB-KW
G protein-coupled receptor signaling pathway Source: GO_Central
Immune response Source: ProtInc
Inflammatory response Source: GO_Central
Killing of cells of other organism Source: UniProtKB
Lymphocyte chemotaxis Source: GO_Central
Monocyte chemotaxis Source: GO_Central
Neutrophil chemotaxis Source: GO_Central
Positive regulation of ERK1 and ERK2 cascade Source: GO_Central
Positive regulation of GTPase activity Source: GO_Central
Positive regulation of T cell migration Source: MGI
Signal transduction Source: ProtInc
T cell migration Source: UniProtKB
Thymocyte migration Source: UniProtKB
Cellular Location
Secreted
PTM
C-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes.

Kwantwi, L. B., Wang, S., Sheng, Y., & Wu, Q. (2021). Multifaceted roles of CCL20 (CC motif chemokine ligand 20): mechanisms and communication networks in breast cancer progression. Bioengineered, 12(1), 6923-6934.

Kwantwi, L. B., Wang, S., Zhang, W., Peng, W., Cai, Z., Sheng, Y., ... & Wu, Q. (2021). Tumor-associated neutrophils activated by tumor-derived CCL20 (CC motif chemokine ligand 20) promote T cell immunosuppression via programmed death-ligand 1 (PD-L1) in breast cancer. Bioengineered, 12(1), 6996-7006.

Maung, H. M. W., Chan-On, W., Kunkeaw, N., & Khaenam, P. (2020). Common transcriptional programs and the role of chemokine (CC motif) ligand 20 (CCL20) in cell migration of cholangiocarcinoma. EXCLI journal, 19, 154.

Kadomoto, S., Izumi, K., & Mizokami, A. (2020). The CCL20-CCR6 axis in cancer progression. International Journal of Molecular Sciences, 21(15), 5186.

Bshara-Corson, S., Gonzalez, I., & Jameson, J. M. (2020). Role of CC Motif Chemokine Ligand 20 in the Regulation of Epidermal γδ T Cells.

Hanson, A., Piras, I. S., Wilhelmsen, D., Still, C. D., Chu, X., Petrick, A., ... & DiStefano, J. K. (2019). Chemokine ligand 20 (CCL20) expression increases with NAFLD stage and hepatic stellate cell activation and is regulated by miR-590-5p. Cytokine, 123, 154789.

Wei, W., Zhao, X., Zhu, J., Zhang, L., Chen, Y., Zhang, B., ... & Wang, C. (2019). lncRNA‑u50535 promotes the progression of lung cancer by activating CCL20/ERK signaling. Oncology reports, 42(5), 1946-1956.

Skovdahl, H. K., Damås, J. K., Granlund, A. V. B., Østvik, A. E., Doseth, B., Bruland, T., ... & Sandvik, A. K. (2018). CC motif ligand 20 (CCL20) and CC motif chemokine receptor 6 (CCR6) in human peripheral blood mononuclear cells: dysregulated in ulcerative colitis and a potential role for CCL20 in IL-1β release. International journal of molecular sciences, 19(10), 3257.

Chen, W., Qin, Y., Wang, D., Zhou, L., Liu, Y., Chen, S., ... & Liu, S. (2018). CCL20 triggered by chemotherapy hinders the therapeutic efficacy of breast cancer. PLoS biology, 16(7), e2005869.

Zhang, X. P., Hu, Z. J., Meng, A. H., Duan, G. C., Zhao, Q. T., & Yang, J. (2017). Role of CCL20/CCR6 and the ERK signaling pathway in lung adenocarcinoma. Oncology letters, 14(6), 8183-8189.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-CCL20 (AA 27-96) Recombinant Antibody (CBFYM-0922)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare