Rabbit Anti-CCL4 Recombinant Antibody (CBFYC-0464) (CBMAB-C0510-FY)

Name: Rabbit Anti-CCL4 Recombinant Antibody (CBFYC-0464)
SKU: CBMAB-C0510-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

CBFYC-0464

Application

WB, IP, IF

Immunogen

A synthesized peptide derived from human CCL4/MIP1 beta.

Host Species

Rabbit

Specificity

Human, Mouse

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:1,000-1:5,000
IF(ICC)	1:50-1:200
IP	1:50

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4, 50% glycerol

Preservative

0.02% sodium azide

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

C-C Motif Chemokine Ligand 4

Introduction

CCL4 (C-C Motif Chemokine Ligand 4) is a Protein Coding gene. Diseases associated with CCL4 include Pulmonary Tuberculosis and Meningitis. Among its related pathways are PEDF Induced Signaling and Cytokine Signaling in Immune system. Gene Ontology (GO) annotations related to this gene include identical protein binding and chemokine activity. An important paralog of this gene is CCL4L2.

Entrez Gene ID

6351

UniProt ID

P13236

Alternative Names

C-C Motif Chemokine Ligand 4; Small Inducible Cytokine A4 (Homologous To Mouse Mip-1b); Macrophage Inflammatory Protein 1-Beta; Lymphocyte Activation Gene 1 Protein; G-26 T-Lymphocyte-Secreted Protein; Chemokine (C-C Motif) Ligand 4; T-Cell Activation Protein 2; MIP-1-Beta(1-69); MIP-1-Beta; SIS-Gamma; PAT 744; SCYA4; LAG-1; MIP1B

Function

Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B.

Biological Process

Cell adhesion Source: ProtInc
Cell-cell signaling Source: ProtInc
Cellular response to interferon-gamma Source: GO_Central
Cellular response to interleukin-1 Source: GO_Central
Cellular response to tumor necrosis factor Source: GO_Central
Chemokine-mediated signaling pathway Source: GO_Central
Cytokine-mediated signaling pathway Source: Reactome
Eosinophil chemotaxis Source: GO_Central
Establishment or maintenance of cell polarity Source: ProtInc
G protein-coupled receptor signaling pathway Source: GO_Central
Immune response Source: ProtInc
Inflammatory response Source: GO_Central
Lymphocyte chemotaxis Source: GO_Central
Monocyte chemotaxis Source: GO_Central
Negative regulation by host of viral transcription Source: UniProtKB
Neutrophil chemotaxis Source: GO_Central
Positive regulation of calcium ion transport Source: UniProtKB
Positive regulation of calcium-mediated signaling Source: UniProtKB
Positive regulation of ERK1 and ERK2 cascade Source: GO_Central
Positive regulation of GTPase activity Source: GO_Central
Positive regulation of natural killer cell chemotaxis Source: UniProtKB
Response to toxic substance Source: UniProtKB
Response to virus Source: ProtInc
Signal transduction Source: ProtInc

Cellular Location

Secreted

PTM

N-terminal processed form MIP-1-beta(3-69) is produced by proteolytic cleavage after secretion from peripheral blood lymphocytes.

Suzukawa, M., Ohshima, N., Tashimo, H., Asari, I., Kobayashi, N., Shoji, S., ... & Ohta, K. (2020). A Low Serum CCL4/MIP-1β Level May Predict a Severe Asthmatic Responsiveness to Mepolizumab. Internal Medicine, 59(22), 2849-2855.

Kim, M. S., Takahashi, T., Lee, J. Y., Toyama, T., Hoshi, T., Kuge, S., ... & Hwang, G. W. (2019). Methylmercury induces the expression of chemokine CCL4 via SRF activation in C17. 2 mouse neural stem cells. Scientific reports, 9(1), 1-11.

Yang, X. W., Wang, F., Qin, R. Z., Zhou, Q. L., & Huang, H. X. (2019). Elevated serum CCL4/MIP-1β levels in postmenopausal osteoporosis patients are linked with disease severity. Biomarkers in medicine, 13(01), 17-25.

Lien, M. Y., Tsai, H. C., Chang, A. C., Tsai, M. H., Hua, C. H., Wang, S. W., & Tang, C. H. (2018). Chemokine CCL4 induces vascular endothelial growth factor C expression and lymphangiogenesis by miR-195-3p in oral squamous cell carcinoma. Frontiers in immunology, 9, 412.

Takahashi, T., Kim, M. S., Iwai-Shimada, M., Fujimura, M., Toyama, T., Naganuma, A., & Hwang, G. W. (2018). Chemokine CCL4 induced in mouse brain has a protective role against methylmercury toxicity. Toxics, 6(3), 36.

Li, L., Liu, Y. D., Zhan, Y. T., Zhu, Y. H., Li, Y., Xie, D., & Guan, X. Y. (2018). High levels of CCL2 or CCL4 in the tumor microenvironment predict unfavorable survival in lung adenocarcinoma. Thoracic cancer, 9(7), 775-784.

Hu, G. N., Tzeng, H. E., Chen, P. C., Wang, C. Q., Zhao, Y. M., Wang, Y., ... & Tang, C. H. (2018). Correlation between CCL4 gene polymorphisms and clinical aspects of breast cancer. International journal of medical sciences, 15(11), 1179.

Kochumon, S., Wilson, A., Chandy, B., Shenouda, S., Tuomilehto, J., Sindhu, S., & Ahmad, R. (2018). Palmitate activates CCL4 expression in human monocytic cells via TLR4/MyD88 dependent activation of NF-κB/MAPK/PI3K signaling systems. Cellular Physiology and Biochemistry, 46(3), 953-964.

Lim, W., Bae, H., Bazer, F. W., & Song, G. (2018). Characterization of CC motif chemokine ligand 4 in the porcine endometrium during the presence of the maternal–fetal interface. Developmental biology, 441(1), 146-158.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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