Mouse Anti-CCNT2 Recombinant Antibody (2128C1a) (CBMAB-C0612-LY)

Name: Mouse Anti-CCNT2 Recombinant Antibody (2128C1a)
SKU: CBMAB-C0612-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

2128C1a

Application

WB, IP

Immunogen

Recombinant protein corresponding to an internal region of cyclin T2a/b of human origin.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.1 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

cyclin T2

Introduction

CCNT2 (Cyclin T2) is a Protein Coding gene. Diseases associated with CCNT2 include Human Immunodeficiency Virus Type 1. Among its related pathways are Formation of HIV elongation complex in the absence of HIV Tat and Gene Expression. Gene Ontology (GO) annotations related to this gene include protein kinase binding. An important paralog of this gene is CCNT1.

Entrez Gene ID

905

UniProt ID

O60583

Alternative Names

Cyclin T2; CYCT2; Subunit Of Positive Elongation Transcription Factor B; SDS-Stable Vimentin-Bound DNA Fragment HEF42VIM22; Cyclin T2a; Cyclin T2b; Cyclin-T2;

Function

Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin T) complex, also called positive transcription elongation factor B (P-TEFB), which is proposed to facilitate the transition from abortive to production elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNAP II) (PubMed:9499409, PubMed:15563843).
The activity of this complex is regulated by binding with 7SK snRNA (PubMed:11713533).
Plays a role during muscle differentiation; P-TEFB complex interacts with MYOD1; this tripartite complex promotes the transcriptional activity of MYOD1 through its CDK9-mediated phosphorylation and binds the chromatin of promoters and enhancers of muscle-specific genes; this event correlates with hyperphosphorylation of the CTD domain of RNA pol II (By similarity).
In addition, enhances MYOD1-dependent transcription through interaction with PKN1 (PubMed:16331689).
Involved in early embryo development (By similarity).
(Microbial infection) Promotes transcriptional activation of early and late herpes simplex virus 1/HHV-1 promoters.

Biological Process

Cell cycle Source: UniProtKB-KW
Cell division Source: UniProtKB-KW
Early viral transcription Source: UniProtKB
Late viral transcription Source: UniProtKB
Positive regulation of DNA-templated transcription, elongation Source: GO_Central
Positive regulation of transcription by RNA polymerase II Source: UniProtKB
Regulation of cyclin-dependent protein serine/threonine kinase activity Source: ProtInc
Regulation of muscle cell differentiation Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: GO_Central
Skeletal muscle tissue development Source: Ensembl
snRNA transcription by RNA polymerase II Source: Reactome
Transcription by RNA polymerase II Source: Reactome
Transcription elongation from RNA polymerase II promoter Source: Reactome

Cellular Location

Nucleus; Perinuclear region. Nucleus in differentiating cells.

Tian, R., Guan, X., Qian, H., Wang, L., Shen, Z., Fang, L., & Liu, Z. (2021). Restoration of NRF2 attenuates myocardial ischemia reperfusion injury through mediating microRNA‐29a‐3p/CCNT2 axis. Biofactors.

Xing, L., Ren, J., Guo, X., Qiao, S., & Tian, T. (2020). Decitabine shows anti-acute myeloid leukemia potential via regulating the miR-212-5p/CCNT2 axis. Open Life Sciences, 15(1), 1013-1023.

Chen, X., Zhang, L., Song, Q., & Chen, Z. (2020). MicroRNA-216b regulates cell proliferation, invasion and cycle progression via interaction with cyclin T2 in gastric cancer. Anti-cancer drugs, 31(6), 623-631.

Wang, F., Zhao, Q. H., Liu, J. Z., & Kong, D. L. (2020). MiRNA-188-5p alleviates the progression of osteosarcoma via target degrading CCNT2. Eur Rev Med Pharmacol Sci, 24(1), 29-35.

Ke, S., Li, R. C., Lu, J., Meng, F. K., Feng, Y. K., & Fang, M. H. (2017). MicroRNA-192 regulates cell proliferation and cell cycle transition in acute myeloid leukemia via interaction with CCNT2. International journal of hematology, 106(2), 258-265.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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