Rat Anti-CCR1 Recombinant Antibody (643854) (CBMAB-C9916-LY)

Name: Rat Anti-CCR1 Recombinant Antibody (643854)
SKU: CBMAB-C9916-LY
Rating: 5 (1 reviews)

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Published Data

Rat Anti-CCR1 Recombinant Antibody (643854) (CBMAB-C9916-LY) in WB

(A and B) CCR1 protein level in (A) BMDMs incubated with rmCCL7(100 ng/ml) and (B) abdominal aortas of saline-infused (n = 3) and Ang II-infused (n = 3) mice. ...View More

Xie, C., Ye, F., Zhang, N., Huang, Y., Pan, Y., & Xie, X. (2021). CCL7 contributes to angiotensin II-induced abdominal aortic aneurysm by promoting macrophage infiltration and pro-inflammatory phenotype. Journal of Cellular and Molecular Medicine, 25(15), 7280-7293.

Rat Anti-CCR1 Recombinant Antibody (643854) (CBMAB-C9916-LY) in FC

WT leukocytes were isolated from the peritoneal cavity 4 hours after thioglycollate injection. 2.9 million cells were injected into WT and CCR1−/− mice and immediately tested in the acetic acid-induced writhing model (n = 5-10). ...View More

Lewis, N. D., Muthukumarana, A., Fogal, S. E., Corradini, L., Stefanopoulos, D. E., Adusumalli, P., ... & Brown, M. L. (2014). CCR1 plays a critical role in modulating pain through hematopoietic and non-hematopoietic cells. PloS one, 9(8), e105883.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rat

Clone

643854

Application

FC, WB

Immunogen

E. coli-derived recombinant mouse CCR1, Extracellular regions (aa 1-34, aa 92-107, aa 172-192, aa 265-281).

Host Species

Rat

Specificity

Mouse

Antibody Isotype

IgG2b

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	2 µg/ml
FC	2.5 µg/10^6 cells

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

PBS, Trehalose

Preservative

None

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

C-C Motif Chemokine Receptor 1

Introduction

CCR1 (C-C Motif Chemokine Receptor 1) is a Protein Coding gene. Diseases associated with CCR1 include Immune-Complex Glomerulonephritis and Chromoblastomycosis. Among its related pathways are Cytokine Signaling in Immune system and Akt Signaling. Gene Ontology (GO) annotations related to this gene include G-protein coupled receptor activity and chemokine receptor activity. An important paralog of this gene is CCR3.

Entrez Gene ID

12768

UniProt ID

P51675

Function

Receptor for a C-C type chemokine. Binds to MIP-1-alpha, MIP-1-delta, RANTES, and MCP-3 and, less efficiently, to MIP-1-beta or MCP-1 and subsequently transduces a signal by increasing the intracellular calcium ions level. Responsible for affecting stem cell proliferation.

Biological Process

Calcium ion transport Source: UniProtKB
Calcium-mediated signaling Source: GO_Central
Cell adhesion Source: ProtInc
Cell-cell signaling Source: UniProtKB
Cell chemotaxis Source: GO_Central
Cell surface receptor signaling pathway Source: ProtInc
Cellular calcium ion homeostasis Source: UniProtKB
Chemokine-mediated signaling pathway Source: UniProtKB
Chemotaxis Source: UniProtKB
Cytokine-mediated signaling pathway Source: Reactome
Dendritic cell chemotaxis Source: BHF-UCL
Exocytosis Source: UniProtKB
G protein-coupled receptor signaling pathway Source: Reactome
G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger Source: ProtInc
Immune response Source: UniProtKB
Inflammatory response Source: GO_Central
Negative regulation of bone mineralization Source: UniProtKB
Negative regulation of gene expression Source: UniProtKB
Positive regulation of calcium ion transport Source: UniProtKB
Positive regulation of cell migration Source: CACAO
Positive regulation of cytosolic calcium ion concentration Source: UniProtKB
Positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
Positive regulation of monocyte chemotaxis Source: UniProtKB
Positive regulation of osteoclast differentiation Source: UniProtKB
Response to wounding Source: ProtInc

Cellular Location

Cell membrane

Topology

Extracellular: 1-34
Helical: 35-60
Cytoplasmic: 61-64
Helical: 65-91
Extracellular: 92-107
Helical: 108-129
Cytoplasmic: 130-146
Helical: 147-171
Extracellular: 172-197
Helical: 198-223
Cytoplasmic: 224-239
Helical: 240-264
Extracellular: 265-281
Helical: 282-305
Cytoplasmic: 306-355

Terasaki, M., Ono, S., Hashimoto, S., Kubota, A., Kojima, H., Ohta, T., ... & Mutoh, M. (2022). Suppression of CC chemokine receptor 1 is a key regulation for colon cancer chemoprevention in AOM/DSS mice by fucoxanthin. The Journal of Nutritional Biochemistry, 99, 108871.

Chou, P. H., Chee, A., Shi, P., Lin, C. L., Zhao, Y., Zhang, L., & An, H. S. (2020). Small molecule antagonist of CC chemokine receptor 1 (CCR1) reduces disc inflammation in the rabbit model. The Spine Journal, 20(12), 2025-2036.

Feng, S., Ju, L., Shao, Z., Grzanna, M., Jia, L., & Liu, M. (2020). Therapeutic Effect of CC Chemokine Receptor Type 1 (CCR1) Antagonist BX471 on Allergic Rhinitis. Journal of Inflammation Research, 13, 343.

Pawlik, K., Piotrowska, A., Kwiatkowski, K., Ciapała, K., Popiolek‐Barczyk, K., Makuch, W., & Mika, J. (2020). The blockade of CC chemokine receptor type 1 influences the level of nociceptive factors and enhances opioid analgesic potency in a rat model of neuropathic pain. Immunology, 159(4), 413-428.

Sanchez, J., e Huma, Z., Lane, J. R., Liu, X., Bridgford, J. L., Payne, R. J., ... & Stone, M. J. (2019). Evaluation and extension of the two-site, two-step model for binding and activation of the chemokine receptor CCR1. Journal of Biological Chemistry, 294(10), 3464-3475.

Sanchez, J., Lane, J. R., Canals, M., & Stone, M. J. (2019). Influence of chemokine N-terminal modification on biased agonism at the chemokine receptor CCR1. International journal of molecular sciences, 20(10), 2417.

Shin, S. Y., Da Hyun Lee, J. L., Choi, C., Kim, J. Y., Nam, J. S., Lim, Y., & Lee, Y. H. (2017). CC motif chemokine receptor 1 (CCR1) is a target of the EGF-AKT-mTOR-STAT3 signaling axis in breast cancer cells. Oncotarget, 8(55), 94591.

Lionakis, M. S., Albert, N. D., Swamydas, M., Lee, C. C. R., Loetscher, P., & Kontoyiannis, D. P. (2017). Pharmacological blockade of the chemokine receptor CCR1 protects mice from systemic candidiasis of hematogenous origin. Antimicrobial agents and chemotherapy, 61(3), e02365-16.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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