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Rat Anti-CCRL2 Recombinant Antibody (498321) (CBMAB-C1365-LY)

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Published Data
CBMAB-C1365-LY-1
Rat Anti-CCRL2 Recombinant Antibody (498321) (CBMAB-C1365-LY) in FC
Biotin-labeled murine ligands were incubated with HEK-muCCRL2 cells for flow cytometry binding experiments. ...View More
Su, Z., Brooks, J., Pelker, J., Andreyeva, T., Sobon, H., Gifford, R., ... & Brennan, J. J. (2023). Studies with neutralizing antibodies suggest CXCL8-mediated neutrophil activation is independent of CC motif chemokine receptor-like 2 (CCRL2) ligand binding function. PLoS One, 18(1), e0280590. Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-C1365-LY-2
Rat Anti-CCRL2 Recombinant Antibody (498321) (CBMAB-C1365-LY) in Neutralization
Effect of CCRL2 neutralization on primary human neutrophil degranulation.
Representative histogram quantifying CCRL2 expression on isolated primary human neutrophils using PE-conjugated K097F7 (red) and is representative of neutrophils profiled from 4 human donors. CCRL2 specific staining was determined by incubating neutrophils with unlabeled K097F7 prior to addition of PE-labelled K097F7 (green) and was compared to isotype control staining (black segmented). ...View More
Su, Z., Brooks, J., Pelker, J., Andreyeva, T., Sobon, H., Gifford, R., ... & Brennan, J. J. (2023). Studies with neutralizing antibodies suggest CXCL8-mediated neutrophil activation is independent of CC motif chemokine receptor-like 2 (CCRL2) ligand binding function. PLoS One, 18(1), e0280590. Distributed under Open Access license CC BY 4.0, without modification.
Datasheet
SDS
Request for COA
Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal
Rat
Clone
498321
Application
FC, ICC
Immunogen
RBL-1 rat basophilic leukemia cell line transfected with mouse CCRL2/LCCR.
Host Species
Rat
Specificity
Mouse
Antibody Isotype
IgG1
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
FC2.5 µg/10^6 cells
IF(ICC)8-25 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Buffer
PBS, Trehalose
Preservative
None
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation

Target

Full Name
C-C Motif Chemokine Receptor Like 2
Introduction
CCRL2 (C-C Motif Chemokine Receptor Like 2) is a Protein Coding gene. Among its related pathways are Akt Signaling and Peptide ligand-binding receptors. Gene Ontology (GO) annotations related to this gene include G-protein coupled receptor activity and chemokine receptor binding.
Entrez Gene ID
54199
UniProt ID
O35457
Function
Receptor for CCL19 and chemerin/RARRES2. Does not appear to be a signaling receptor, but may have a role in modulating chemokine-triggered immune responses by capturing and internalizing CCL19 or by presenting RARRES2 ligand to CMKLR1, a functional signaling receptors. Plays a critical role for the development of Th2 responses.
Biological Process
Calcium-mediated signaling Source: GO_Central
Cell chemotaxis Source: GO_Central
Chemotaxis Source: ProtInc
G protein-coupled receptor signaling pathway Source: ProtInc
Immune response Source: GO_Central
Inflammatory response Source: UniProtKB
Positive regulation of cytosolic calcium ion concentration Source: GO_Central
Cellular Location
Cell membrane
Topology
Extracellular: 1-43
Helical: 44-64
Cytoplasmic: 65-74
Helical: 75-95
Extracellular: 96-104
Helical: 105-125
Cytoplasmic: 126-144
Helical: 145-165
Extracellular: 166-198
Helical: 199-219
Cytoplasmic: 220-238
Helical: 239-259
Extracellular: 260-286
Helical: 287-307
Cytoplasmic: 308-344

Islam, S., Moni, M. A., Urmi, U. L., Tanaka, A., & Hoshino, H. (2021). CC Chemokine receptor-like 2 (CCRL2) acts as coreceptor for human immunodeficiency virus-2. Briefings in Bioinformatics, 22(4), bbaa333.

Yin, W., Li, Y., Song, Y., Zhang, J., Wu, C., Chen, Y., ... & He, R. (2021). CCRL2 promotes antitumor T-cell immunity via amplifying TLR4-mediated immunostimulatory macrophage activation. Proceedings of the National Academy of Sciences, 118(16).

Schioppa, T., Sozio, F., Barbazza, I., Scutera, S., Bosisio, D., Sozzani, S., & Del Prete, A. (2020). Molecular basis for CCRL2 regulation of leukocyte migration. Frontiers in Cell and Developmental Biology, 8, 1570.

Zhang, Y., Li, S., Liu, Q., Long, R., Feng, J., Qin, H., ... & Luo, J. (2020). Mycobacterium tuberculosis heat-shock protein 16.3 induces macrophage M2 polarization through CCRL2/CX3CR1. Inflammation, 43(2), 487-506.

Xu, M., Wang, Y. M., Li, W. Q., Huang, C. L., Li, J., Xie, W. H., ... & Li, X. (2020). Ccrl2 deficiency deteriorates obesity and insulin resistance through increasing adipose tissue macrophages infiltration. Genes & Diseases.

Reyes, N., Benedetti, I., Rebollo, J., Correa, O., & Geliebter, J. (2019). Atypical chemokine receptor CCRL2 is overexpressed in prostate cancer cells. Journal of biomedical research, 33(1), 17.

Del Prete, A., Sozio, F., Schioppa, T., Ponzetta, A., Vermi, W., Calza, S., ... & Sozzani, S. (2019). The atypical receptor CCRL2 is essential for NK cell-dependent resistance against lung cancer. Cancer immunology research, 7(11), 1775.

Mazzotti, C., Gagliostro, V., Bosisio, D., Del Prete, A., Tiberio, L., Thelen, M., & Sozzani, S. (2017). The atypical receptor CCRL2 (CC Chemokine Receptor-Like 2) does not act as a decoy receptor in endothelial cells. Frontiers in immunology, 8, 1233.

Zimny, S., Pohl, R., Rein-Fischboeck, L., Haberl, E. M., Krautbauer, S., Weiss, T. S., & Buechler, C. (2017). Chemokine (CC-motif) receptor-like 2 mRNA is expressed in hepatic stellate cells and is positively associated with characteristics of non-alcoholic steatohepatitis in mice and men. Experimental and molecular pathology, 103(1), 1-8.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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