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Mouse Anti-CCT3 Recombinant Antibody (CF065) (CBMAB-FT392LY)


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Published Data
CBMAB-FT392LY-1
Mouse Anti-CCT3 Recombinant Antibody (CF065) (CBMAB-FT392LY) in IHC
Representative IHC images of liver cancer tissues with low and high expression of CCT3, YAP and TFCP2. Scale bar, 500 μm. ...View More
Liu, Y., Zhang, X., Lin, J., Chen, Y., Qiao, Y., Guo, S., ... & Sun, F. (2019). CCT3 acts upstream of YAP and TFCP2 as a potential target and tumour biomarker in liver cancer. Cell death & disease, 10(9), 644. Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-FT392LY-2
Mouse Anti-CCT3 Recombinant Antibody (CF065) (CBMAB-FT392LY) in WB
Representative WB images of YAP/TFCP2 and their targets in control cells, Bel-7402 and SMMC-7721 cells with CCT3 knocked down in the presence or absence of YAP, TFCP2 or PCBP2 overexpression. ...View More
Liu, Y., Zhang, X., Lin, J., Chen, Y., Qiao, Y., Guo, S., ... & Sun, F. (2019). CCT3 acts upstream of YAP and TFCP2 as a potential target and tumour biomarker in liver cancer. Cell death & disease, 10(9), 644. Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-FT392LY-3
Mouse Anti-CCT3 Recombinant Antibody (CF065) (CBMAB-FT392LY) in IF
CCT3 regulated expression but not sub-cellular localisation of YAP and TFCP2, as measured by IF in Bel-7402 cells. Scale bar, 20 μm. ...View More
Liu, Y., Zhang, X., Lin, J., Chen, Y., Qiao, Y., Guo, S., ... & Sun, F. (2019). CCT3 acts upstream of YAP and TFCP2 as a potential target and tumour biomarker in liver cancer. Cell death & disease, 10(9), 644. Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-FT392LY-4
Mouse Anti-CCT3 Recombinant Antibody (CF065) (CBMAB-FT392LY) in IHC
CCT3 was highly upregulated in liver cancer compared to adjacent liver, as evaluated in five paired adjacent (A) and tumourous (T) liver tissue. The representative IHC images of CCT3 are also shown on the right side. Scale bar, 200 μm. ...View More
Liu, Y., Zhang, X., Lin, J., Chen, Y., Qiao, Y., Guo, S., ... & Sun, F. (2019). CCT3 acts upstream of YAP and TFCP2 as a potential target and tumour biomarker in liver cancer. Cell death & disease, 10(9), 644. Distributed under Open Access license CC BY 4.0, without modification.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CF065
Antibody Isotype
IgG1, κ
Application
ELISA, IF, IP, WB

Basic Information

Immunogen
Amino acids 101-400 mapping within an internal region of TCP-1 γ of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Introduction
CCT3 (Chaperonin Containing TCP1 Subunit 3) is a Protein Coding gene. Diseases associated with CCT3 include Spinocerebellar Ataxia 10 and Immature Cataract. Among its related pathways are Organelle biogenesis and maintenance and Chaperonin-mediated protein folding. Gene Ontology (GO) annotations related to this gene include unfolded protein binding.
Entrez Gene ID
Human7203
Mouse12462
UniProt ID
HumanP49368
MouseP80318
Function
Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis (PubMed:25467444).
The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance (PubMed:25467444).
As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia (PubMed:20080638).
The TRiC complex plays a role in the folding of actin and tubulin (Probable).
Biological Process
Binding of sperm to zona pellucida Source: Ensembl
Pore complex assembly Source: Ensembl
Positive regulation of establishment of protein localization to telomere Source: BHF-UCL
Positive regulation of protein localization to Cajal body Source: BHF-UCL
Positive regulation of telomerase RNA localization to Cajal body Source: BHF-UCL
Positive regulation of telomere maintenance via telomerase Source: BHF-UCL
Protein folding Source: FlyBase
Protein stabilization Source: BHF-UCL
Toxin transport Source: Ensembl
Cellular Location
Cytoplasm
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For research use only. Not intended for any clinical use.

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