Mouse Anti-MCAM Recombinant Antibody (
4D10C7) (V2LY-0725-LY166)

Name: Mouse Anti-MCAM Recombinant Antibody (4D10C7)
SKU: V2LY-0725-LY166
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-MCAM Recombinant Antibody (4D10C7) (V2LY-0725-LY166) in WB

Western Blot
Positive WB detected in: MCF-7 whole cell lysate
All lanes: CD146 antibody at 1:1250
Secondary
Goat polyclonal to Mouse IgG at 1/50000 dilution
Predicted band size: 72, 58 kDa
Observed band size: 120 kDa

Mouse Anti-MCAM Recombinant Antibody (4D10C7) (V2LY-0725-LY166) in WB

Western Blot
Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate
All lanes: CD146 antibody at 1:500
Secondary
Goat polyclonal to Mouse IgG at 1/50000 dilution
Predicted band size: 72, 58 kDa
Observed band size: 120 kDa

Mouse Anti-MCAM Recombinant Antibody (4D10C7) (V2LY-0725-LY166) in IF

Immunofluorescence staining of A375 cells with V2LY-0725-LY166 at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

Mouse Anti-MCAM Recombinant Antibody (4D10C7) (V2LY-0725-LY166) in IF

Immunofluorescence staining of Hela cells with V2LY-0725-LY166 at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

Mouse Anti-MCAM Recombinant Antibody (4D10C7) (V2LY-0725-LY166) in IF

Immunofluorescence staining of MCF-7 cells with V2LY-0725-LY166 at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

Mouse Anti-MCAM Recombinant Antibody (4D10C7) (V2LY-0725-LY166) in IF

Immunofluorescence staining of THP-1 cells with V2LY-0725-LY166 at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

Mouse Anti-MCAM Recombinant Antibody (4D10C7) (V2LY-0725-LY166) in FC

Overlay histogram showing MCF-7 cells stained with V2LY-0725-LY166 (red line) at 1:400. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of>10,000 events was performed.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

4D10C7

Application

ELISA, WB, IF, FC

Immunogen

Recombinant Human Cell surface glycoprotein MUC18 protein (50-646AA).

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
IF	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, Glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Melanoma Cell Adhesion Molecule

Entrez Gene ID

4162

UniProt ID

P43121

Function

Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.

Biological Process

Anatomical structure morphogenesis Source: ProtInc
Angiogenesis Source: MGI
Cell adhesion Source: ProtInc
Glomerular filtration Source: UniProtKB
Positive regulation of cell migration Source: Ensembl
Vascular wound healing Source: UniProtKB

Cellular Location

Membrane

Topology

Extracellular: 24-559
Helical: 560-583
Cytoplasmic: 584-646

Pang, Y., Maxwell, E., Sindrewicz-Goral, P., Shapanis, A., Li, S., Morgan, M., & Yu, L. G. (2022). Galectin-3 Is a Natural Binding Ligand of MCAM (CD146, MUC18) in Melanoma Cells and Their Interaction Promotes Melanoma Progression. Biomolecules, 12(10), 1451.

Ikeguchi, R., Shimizu, Y., Kondo, A., Kanda, N., So, H., Kojima, H., & Kitagawa, K. (2021). Melanoma cell adhesion molecule expressing helper T cells in CNS inflammatory demyelinating diseases. Neurology-Neuroimmunology Neuroinflammation, 8(6).

Wang, Z., Xu, Q., Zhang, N., Du, X., Xu, G., & Yan, X. (2020). CD146, from a melanoma cell adhesion molecule to a signaling receptor. Signal transduction and targeted therapy, 5(1), 148.

Petersen, E. R., Ammitzbøll, C., Søndergaard, H. B., Oturai, A. B., Sørensen, P. S., Nilsson, A. C., ... & Sellebjerg, F. (2019). Expression of melanoma cell adhesion molecule-1 (MCAM-1) in natalizumab-treated multiple sclerosis. Journal of Neuroimmunology, 337, 577085.

Chen, Y., Sumardika, I. W., Tomonobu, N., Ruma, I. M. W., Kinoshita, R., Kondo, E., ... & Sakaguchi, M. (2019). Melanoma cell adhesion molecule is the driving force behind the dissemination of melanoma upon S100A8/A9 binding in the original skin lesion. Cancer Letters, 452, 178-190.

Čorić, M. (2019). Development and characterisation of monoclonal antibodies to tumour endothelial marker ‘melanoma cell adhesion molecule’(MCAM) and approaches to targeting MCAM on tumour vessels (Doctoral dissertation, University of Birmingham).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Anti-MCAM Recombinant Antibody (4D10C7) (CAT#: CBMAB-R4323-CN)

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Isotype control

For research use only. Not intended for any clinical use.

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