Rabbit Anti-CD163 Recombinant Antibody (CBNH-018) (CBMAB-R4343-CN)

Name: Rabbit Anti-CD163 Recombinant Antibody (CBNH-018)
SKU: CBMAB-R4343-CN
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

CBNH-018

Application

WB, IHC, FC, IF

Immunogen

Recombinant human CD163 between 1 to 250 amino acids.

Host Species

Rabbit

Specificity

Human, Rat

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:300-1:5,000
IF(ICC)	1:50-1:200
IHC-P	1:200-1:400
FC	1:20-1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

TBS, pH7.4, 1%BSA, 40%Glycerol

Preservative

0.05% sodium azide

Concentration

1 mg/ml

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

More Infomation

Target

Full Name

CD163 Molecule

Introduction

CD163 (CD163 Molecule) is a Protein Coding gene. Diseases associated with CD163 include Rosai-Dorfman Disease and Non-Langerhans-Cell Histiocytosis. Among its related pathways are Hematopoietic Stem Cell Differentiation Pathways and Lineage-specific Markers and Binding and Uptake of Ligands by Scavenger Receptors. Gene Ontology (GO) annotations related to this gene include scavenger receptor activity. An important paralog of this gene is CD163L1.

Entrez Gene ID

8350

UniProt ID

P68431

Function

Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.
After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.

Biological Process

Acute-phase response Source: UniProtKB-KW
Negative regulation of inflammatory response to antigenic stimulus Source: Reactome
Receptor-mediated endocytosis Source: Reactome

Cellular Location

Secreted; Cell membrane. Isoform 1 and isoform 2 show a lower surface expression when expressed in cells.

Topology

Extracellular: 42-1050
Helical: 1051-1071
Cytoplasmic: 1072-1156

PTM

A soluble form (sCD163) is produced by proteolytic shedding which can be induced by lipopolysaccharide, phorbol ester and Fc region of immunoglobulin gamma. This cleavage is dependent on protein kinase C and tyrosine kinases and can be blocked by protease inhibitors. The shedding is inhibited by the tissue inhibitor of metalloproteinase TIMP3, and thus probably induced by membrane-bound metalloproteinases ADAMs.
Phosphorylated.

Huang, C., Bernard, D., Zhu, J., Dash, R. C., Chu, A., Knupp, A., ... & Tang, Y. (2020). Small molecules block the interaction between porcine reproductive and respiratory syndrome virus and CD163 receptor and the infection of pig cells. Virology journal, 17(1), 1-11.

Zhang, Y., Zhang, K., Zheng, H., Liu, C., Jiang, Y., Du, N., ... & Gao, F. (2020). Development of a monoclonal antibody against porcine CD163 SRCR5 domain which partially blocks infection of PRRSV. Frontiers in Veterinary Science, 7, 923.

Kowalska, W. (2020). Expression of CD163 and HLA-DR molecules on the monocytes in chronic lymphocytic leukemia patients. Folia histochemica et cytobiologica, 58(1), 17-24.

Fujiwara, Y., Ohnishi, K., Horlad, H., Saito, Y., Shiraishi, D., Takeya, H., ... & Komohara, Y. (2020). CD163 deficiency facilitates lipopolysaccharide‐induced inflammatory responses and endotoxin shock in mice. Clinical & translational immunology, 9(9), e1162.

Nielsen, M. C., Hvidbjerg Gantzel, R., Clària, J., Trebicka, J., Møller, H. J., & Grønbæk, H. (2020). Macrophage activation markers, CD163 and CD206, in acute-on-chronic liver failure. Cells, 9(5), 1175.

Nunes, P. R., Romão-Veiga, M., Peraçoli, J. C., Costa, R. A. A., de Oliveira, L. G., Borges, V. T. M., & Peraçoli, M. T. (2019). Downregulation of CD163 in monocytes and its soluble form in the plasma is associated with a pro-inflammatory profile in pregnant women with preeclampsia. Immunologic research, 67(2), 194-201.

Liu, S., Zhang, C., Maimela, N. R., Yang, L., Zhang, Z., Ping, Y., ... & Zhang, Y. (2019). Molecular and clinical characterization of CD163 expression via large-scale analysis in glioma. Oncoimmunology, 8(7), e1601478.

Shiraishi, D., Fujiwara, Y., Horlad, H., Saito, Y., Iriki, T., Tsuboki, J., ... & Komohara, Y. (2018). CD163 is required for protumoral activation of macrophages in human and murine sarcoma. Cancer research, 78(12), 3255-3266.

Cheng, Z., Zhang, D., Gong, B., Wang, P., & Liu, F. (2017). CD163 as a novel target gene of STAT3 is a potential therapeutic target for gastric cancer. Oncotarget, 8(50), 87244.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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