Rabbit Anti-CD19 Recombinant Antibody (
4C7) (V2LY-0725-LY1352)

Name: Rabbit Anti-CD19 Recombinant Antibody (4C7)
SKU: V2LY-0725-LY1352
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-CD19 Recombinant Antibody (4C7) (V2LY-0725-LY1352) in WB

Western Blot
Positive WB detected in: Daudi whole cell lysate, Raji whole cell lysate
All lanes: CD19 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 62 kDa
Observed band size: 95 kDa

Rabbit Anti-CD19 Recombinant Antibody (4C7) (V2LY-0725-LY1352) in IHC

IHC image of V2LY-0725-LY1352 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Rabbit Anti-CD19 Recombinant Antibody (4C7) (V2LY-0725-LY1352) in IP

Immunoprecipitating CD19 in Raji whole cell lysate
Lane 1: Rabbit control IgG instead of V2LY-0725-LY1352 in Raji whole cell lysate.
For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: V2LY-0725-LY1352(2µg)+ Raji whole cell lysate(500µg)
Lane 3: Raji whole cell lysate (10µg)

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

4C7

Application

ELISA, WB, IHC, IP

Immunogen

A synthesized peptide derived from human CD19.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:5,000
IHC	1:50-1:200
IP	1:200-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

CD19 Molecule

Entrez Gene ID

930

UniProt ID

P15391

Alternative Names

CD19 Molecule; B-Lymphocyte Surface Antigen B4; T-Cell Surface Antigen Leu-12; Differentiation Antigen CD19; CD19 Antigen; B-Lymphocyte Antigen CD19; CVID3; B4;

Function

Functions as coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens (PubMed:2463100, PubMed:1373518, PubMed:16672701).
Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca2+ stores (PubMed:9382888, PubMed:9317126, PubMed:12387743, PubMed:16672701).
Is not required for early steps during B cell differentiation in the blood marrow (PubMed:9317126).
Required for normal differentiation of B-1 cells (By similarity).
Required for normal B cell differentiation and proliferation in response to antigen challenges (PubMed:2463100, PubMed:1373518).
Required for normal levels of serum immunoglobulins, and for production of high-affinity antibodies in response to antigen challenge (PubMed:9317126, PubMed:12387743, PubMed:16672701).

Biological Process

Antigen receptor-mediated signaling pathway Source: UniProtKB
B-1 B cell differentiation Source: Ensembl
B cell proliferation involved in immune response Source: UniProtKB
B cell receptor signaling pathway Source: UniProtKB
Immunoglobulin mediated immune response Source: UniProtKB
Positive regulation of phosphatidylinositol 3-kinase activity Source: UniProtKB
Positive regulation of protein kinase B signaling Source: Reactome
Positive regulation of release of sequestered calcium ion into cytosol Source: UniProtKB
Regulation of B cell activation Source: UniProtKB
Regulation of B cell receptor signaling pathway Source: UniProtKB
Regulation of complement activation Source: Reactome
Regulation of immune response Source: Reactome

Cellular Location

Cell membrane; Membrane raft

Involvement in disease

Immunodeficiency, common variable, 3 (CVID3): A primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low.

Topology

Extracellular: 20-291
Helical: 292-313
Cytoplasmic: 314-556

PTM

Phosphorylated on tyrosine following B-cell activation (PubMed:7684160, PubMed:7687539, PubMed:10706702, PubMed:12387743). Phosphorylated on tyrosine residues by LYN (PubMed:7687428). Tyrosine residues are phosphorylated sequentially after activation of the B cell receptor. Phosphorylation of Tyr-531 is extremely rapid, followed by phosphorylation at Tyr-409. In contrast, phosphorylation of Tyr-500 appears more slowly and is more transient, returning rapidly to basal levels (By similarity).

Spiegel, J. Y., Patel, S., Muffly, L., Hossain, N. M., Oak, J., Baird, J. H., ... & Miklos, D. B. (2021). CAR T cells with dual targeting of CD19 and CD22 in adult patients with recurrent or refractory B cell malignancies: a phase 1 trial. Nature Medicine, 27(8), 1419-1431.

Nerreter, T., Letschert, S., Götz, R., Doose, S., Danhof, S., Einsele, H., ... & Hudecek, M. (2019). Super-resolution microscopy reveals ultra-low CD19 expression on myeloma cells that triggers elimination by CD19 CAR-T. Nature communications, 10(1), 1-11.

Ying, Z., Huang, X. F., Xiang, X., Liu, Y., Kang, X., Song, Y., ... & Chen, S. Y. (2019). A safe and potent anti-CD19 CAR T cell therapy. Nature medicine, 25(6), 947-953.

Hatterer, E., Barba, L., Noraz, N., Daubeuf, B., Aubry-Lachainaye, J. P., von der Weid, B., ... & Buatois, V. (2019, February). Co-engaging CD47 and CD19 with a bispecific antibody abrogates B-cell receptor/CD19 association leading to impaired B-cell proliferation. In MAbs (Vol. 11, No. 2, pp. 322-334). Taylor & Francis.

Salter, A. I., Pont, M. J., & Riddell, S. R. (2018). Chimeric antigen receptor–modified T cells: CD19 and the road beyond. Blood, The Journal of the American Society of Hematology, 131(24), 2621-2629.

Bagashev, A., Sotillo, E., Tang, C. H. A., Black, K. L., Perazzelli, J., Seeholzer, S. H., ... & Thomas-Tikhonenko, A. (2018). CD19 alterations emerging after CD19-directed immunotherapy cause retention of the misfolded protein in the endoplasmic reticulum. Molecular and cellular biology, 38(21), e00383-18.

Turtle, C. J., Hay, K. A., Hanafi, L. A., Li, D., Cherian, S., Chen, X., ... & Maloney, D. G. (2017). Durable molecular remissions in chronic lymphocytic leukemia treated with CD19-specific chimeric antigen receptor–modified T cells after failure of ibrutinib. Journal of Clinical Oncology, 35(26), 3010.

Alabanza, L., Pegues, M., Geldres, C., Shi, V., Wiltzius, J. J., Sievers, S. A., ... & Kochenderfer, J. N. (2017). Function of novel anti-CD19 chimeric antigen receptors with human variable regions is affected by hinge and transmembrane domains. Molecular Therapy, 25(11), 2452-2465.

Li, X., Ding, Y., Zi, M., Sun, L., Zhang, W., Chen, S., & Xu, Y. (2017). CD19, from bench to bedside. Immunology letters, 183, 86-95.

Liu, L., Lam, C. Y. K., Long, V., Widjaja, L., Yang, Y., Li, H., ... & Bonvini, E. (2017). MGD011, A CD19 x CD3 dual-affinity retargeting bi-specific molecule incorporating extended circulating half-life for the treatment of B-cell malignancies. Clinical Cancer Research, 23(6), 1506-1518.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

CD19 Matched Antibody Pair (207) (CAT#: APMAB-207LY)

CD19 Matched Antibody Pair (208) (CAT#: APMAB-208LY)

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