Rat Anti-CD226 Recombinant Antibody (10E5) (CBMAB-C0183-LY)

Name: Rat Anti-CD226 Recombinant Antibody (10E5)
SKU: CBMAB-C0183-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rat

Clone

10E5

Application

Immunogen

Th1 polarized T cell clones.

Host Species

Rat

Specificity

Mouse

Antibody Isotype

IgG2b

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

0.09% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

CD226 Molecule

Introduction

CD226 (CD226 Molecule) is a Protein Coding gene. Diseases associated with CD226 include Ovarian Cystic Teratoma and Mature Teratoma Of The Ovary. Among its related pathways are Hematopoietic Stem Cell Differentiation Pathways and Lineage-specific Markers and Innate Immune System. Gene Ontology (GO) annotations related to this gene include protein kinase binding and cell adhesion molecule binding.

Entrez Gene ID

225825

UniProt ID

Q8K4E3

Alternative Names

CD226 Molecule; CD226 Antigen; DNAM-1; DNAM1; T Lineage-Specific Activation Antigen 1 Antigen; Platelet And T Cell Activation Antigen 1;

Function

Involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cell (PubMed:8673704).
Cell surface receptor for NECTIN2. Upon ligand binding, stimulates T-cell proliferation and cytokine production, including that of IL2, IL5, IL10, IL13, and IFNG. Competes with PVRIG for NECTIN2-binding (PubMed:26755705).

Biological Process

Cell adhesion Source: ProtInc
Cell recognition Source: BHF-UCL
Positive regulation of Fc receptor mediated stimulatory signaling pathway Source: BHF-UCL
Positive regulation of immunoglobulin mediated immune response Source: BHF-UCL
Positive regulation of interferon-gamma production Source: GO_Central
Positive regulation of mast cell activation Source: BHF-UCL
Positive regulation of natural killer cell cytokine production Source: GO_Central
Positive regulation of natural killer cell mediated cytotoxicity Source: BHF-UCL
Positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target Source: BHF-UCL
Positive regulation of T cell receptor signaling pathway Source: UniProtKB
Regulation of immune response Source: Reactome
Signal transduction Source: ProtInc

Cellular Location

Cell membrane

Topology

Extracellular: 19-254
Helical: 255-275
Cytoplasmic: 276-336

Bi, J. (2021). CD226: a potent driver of antitumor immunity that needs to be maintained. Cellular & Molecular Immunology, 1-2.

Feng, M., Wu, Z., Zhou, Y., Wei, Z., Tian, E., Mei, S., ... & Zhu, D. (2021). BCL9 regulates CD226 and CD96 checkpoints in CD8+ T cells to improve PD-1 response in cancer. Signal transduction and targeted therapy, 6(1), 1-14.

Huang, Z., Qi, G., Miller, J. S., & Zheng, S. G. (2020). CD226: An emerging role in immunologic diseases. Frontiers in cell and developmental biology, 8, 564.

Wang, N., Yi, H., Fang, L., Jin, J., Ma, Q., Shen, Y., ... & Chen, L. (2020). CD226 attenuates Treg proliferation via Akt and Erk signaling in an EAE model. Frontiers in immunology, 11, 1883.

Shapiro, M. R., Yeh, W. I., Longfield, J. R., Gallagher, J., Infante, C. M., Wellford, S., ... & Brusko, T. M. (2020). CD226 deletion reduces Type 1 diabetes in the NOD mouse by impairing thymocyte development and peripheral T cell activation. Frontiers in immunology, 11, 2180.

Weulersse, M., Asrir, A., Pichler, A. C., Lemaitre, L., Braun, M., Carrié, N., ... & Martinet, L. (2020). Eomes-dependent loss of the co-activating receptor CD226 restrains CD8+ T cell anti-tumor functions and limits the efficacy of cancer immunotherapy. Immunity, 53(4), 824-839.

Wang, H., Qi, J., Zhang, S., Li, Y., Tan, S., & Gao, G. F. (2019). Binding mode of the side-by-side two-IgV molecule CD226/DNAM-1 to its ligand CD155/Necl-5. Proceedings of the National Academy of Sciences, 116(3), 988-996.

Wang, N., Liang, S., Jin, J., Fang, L., Ma, Q., Wang, X., ... & Chen, L. (2019). CD226 attenuates Treg suppressive capacity via CTLA-4 and TIGIT during EAE. Immunologic research, 67(6), 486-496.

Fourcade, J., Sun, Z., Chauvin, J. M., Ka, M., Davar, D., Pagliano, O., ... & Zarour, H. M. (2018). CD226 opposes TIGIT to disrupt Tregs in melanoma. JCI insight, 3(14).

Wagner, A. K., Kadri, N., Snäll, J., Brodin, P., Gilfillan, S., Colonna, M., ... & Chambers, B. J. (2017). Expression of CD226 is associated to but not required for NK cell education. Nature communications, 8(1), 1-14.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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