Mouse Anti-CD44 Recombinant Antibody (DF1485) (CBMAB-C0630-CQ)

Name: Mouse Anti-CD44 Recombinant Antibody (DF1485)
SKU: CBMAB-C0630-CQ
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CD44 Recombinant Antibody (DF1485) (CBMAB-C0630-CQ) in FC

Quantification of CD44+/CD24- subpopulations in MDA-MB-468 and MDA-MB-231 using flow cytometry, relative to TCP control. TNBC cells were cultured for 5 days in reduced serum (2% FBS) on the topographical substrates and TCP (control). ...View More

Rosado-Galindo, H., & Domenech, M. (2023). Surface roughness modulates EGFR signaling and stemness of triple-negative breast cancer cells. Frontiers in Cell and Developmental Biology, 11, 1124250. Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CD44 Recombinant Antibody (DF1485) (CBMAB-C0630-CQ) in WB

CD44 densitometric analysis of the specific Western blot. ...View More

Galla, R., Ruga, S., Aprile, S., Ferrari, S., Brovero, A., Grosa, G., ... & Uberti, F. (2022). New Hyaluronic Acid from Plant Origin to Improve Joint Protection-An In Vitro Study. International Journal of Molecular Sciences, 23(15), 8114. Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CD44 Recombinant Antibody (DF1485) (CBMAB-C0630-CQ) in IF

For confocal microscopy analysis of proteins located in cell-to-cell junctions, cells were stained with anti-Myc antibody (Myc-TRIM40, green) and anti-CD44, anti-E-cadherin, anti-Vinculin, or anti-β-catenin antibodies (AJ proteins, red). ...View More

Kang, S., Kim, J., Park, A., Koh, M., Shin, W., Park, G., ... & Park, B. (2023). TRIM40 is a pathogenic driver of inflammatory bowel disease subverting intestinal barrier integrity. Nature communications, 14(1), 700. Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CD44 Recombinant Antibody (DF1485) (CBMAB-C0630-CQ) in WB

Representative Western blot (n = 2) result of protein lysates (20 µg) of MDA-MB-231-BR WT, control (scramble), and CD44 KD (shCD44) showing expression of the HA receptor CD44. β-Actin was used as a loading control. ...View More

Hamester, F., Stürken, C., Legler, K., Eylmann, K., Möller, K., Roβberg, M., ... & Oliveira-Ferrer, L. (2022). Key role of hyaluronan metabolism for the development of brain metastases in triple-negative breast cancer. Cells, 11(20), 3275. Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

DF1485

Application

IP, IHC-P, FC, WB, IF

Immunogen

Purified CD44 antigen (PGp-1) from lymphocyte membrane.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

CD44 Molecule (Indian Blood Group)

Introduction

The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.

Entrez Gene ID

Human	960
Mouse	12505

UniProt ID

Human	P16070
Mouse	P15379

Alternative Names

CD44 Molecule (Indian Blood Group); Hematopoietic Cell E- And L-Selectin Ligand; GP90 Lymphocyte Homing/Adhesion Receptor; Chondroitin Sulfate Proteoglycan 8; Extracellular Matrix Receptor III; Heparan Sulfate Proteoglycan; Phagocytic Glycoprotein 1; Hyaluronate Receptor; Hermes Antigen; CD44 Antigen; ECMR-III; HUTCH-I; Epican; CDW44; MDU2; MDU3;

Function

Cell-surface receptor that plays a role in cell-cell interactions, cell adhesion and migration, helping them to sense and respond to changes in the tissue microenvironment (PubMed:16541107, PubMed:19703720, PubMed:22726066).
Participates thereby in a wide variety of cellular functions including the activation, recirculation and homing of T-lymphocytes, hematopoiesis, inflammation and response to bacterial infection (PubMed:7528188).
Engages, through its ectodomain, extracellular matrix components such as hyaluronan/HA, collagen, growth factors, cytokines or proteases and serves as a platform for signal transduction by assembling, via its cytoplasmic domain, protein complexes containing receptor kinases and membrane proteases (PubMed:18757307, PubMed:23589287).
Such effectors include PKN2, the RhoGTPases RAC1 and RHOA, Rho-kinases and phospholipase C that coordinate signaling pathways promoting calcium mobilization and actin-mediated cytoskeleton reorganization essential for cell migration and adhesion (PubMed:15123640).

Biological Process

Cartilage development Source: UniProtKB
Cell adhesion Source: UniProtKB
Cell-cell adhesion Source: UniProtKB
Cell-matrix adhesion Source: UniProtKB
Cell migration Source: UniProtKB
Cellular response to fibroblast growth factor stimulus Source: UniProtKB
Extracellular matrix disassembly Source: Reactome
Extracellular matrix organization Source: Reactome
Hyaluronan catabolic process Source: UniProtKB
Inflammatory response Source: GO_Central
Interferon-gamma-mediated signaling pathway Source: Reactome
Leukocyte migration Source: Reactome
Monocyte aggregation Source: UniProtKB
Negative regulation of apoptotic process Source: UniProtKB
Negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
Negative regulation of DNA damage response, signal transduction by p53 class mediator Source: BHF-UCL
Negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator Source: BHF-UCL
Neutrophil degranulation Source: Reactome
Positive regulation of ERK1 and ERK2 cascade Source: BHF-UCL
Positive regulation of heterotypic cell-cell adhesion Source: UniProtKB
Positive regulation of monocyte aggregation Source: BHF-UCL
Positive regulation of peptidyl-serine phosphorylation Source: BHF-UCL
Positive regulation of peptidyl-tyrosine phosphorylation Source: BHF-UCL
Regulation of lamellipodium morphogenesis Source: UniProtKB
T cell activation Source: UniProtKB
Wound healing, spreading of cells Source: UniProtKB

Cellular Location

Cell membrane; Microvillus. Colocalizes with actin in membrane protrusions at wounding edges. Co-localizes with RDX, EZR and MSN in microvilli. Localizes to cholesterol-rich membrane-bound lipid raft domains.

Topology

Extracellular: 21-649
Helical: 650-670
Cytoplasmic: 671-742

PTM

Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
N-glycosylated.
O-glycosylated. O-glycosylation contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s). It is uncertain if O-glycosylation occurs on Thr-637 or Thr-638.
Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.

Liang, Y., Wang, Y., Wang, L., Liang, Z., Li, D., Xu, X., ... & Niu, H. (2021). Self-crosslinkable chitosan-hyaluronic acid dialdehyde nanoparticles for CD44-targeted siRNA delivery to treat bladder cancer. Bioactive materials, 6(2), 433-446.

Al-Othman, N., Alhendi, A., Ihbaisha, M., Barahmeh, M., Alqaraleh, M., & Al-Momany, B. Z. (2020). Role of CD44 in breast cancer. Breast disease, 39(1), 1-13.

Godavarthy, P. S., Kumar, R., Herkt, S. C., Pereira, R. S., Hayduk, N., Weissenberger, E. S., ... & Krause, D. S. (2020). The vascular bone marrow niche influences outcome in chronic myeloid leukemia via the E-selectin-SCL/TAL1-CD44 axis. Haematologica, 105(1), 136.

Zhang, R., Rejeeth, C., Xu, W., Zhu, C., Liu, X., Wan, J., ... & Qian, K. (2019). Label-free electrochemical sensor for cd44 by ligand-protein interaction. Analytical chemistry, 91(11), 7078-7085.

Skandalis, S. S., Karalis, T. T., Chatzopoulos, A., & Karamanos, N. K. (2019). Hyaluronan-CD44 axis orchestrates cancer stem cell functions. Cellular signalling, 63, 109377.

Chen, C., Zhao, S., Karnad, A., & Freeman, J. W. (2018). The biology and role of CD44 in cancer progression: therapeutic implications. Journal of hematology & oncology, 11(1), 1-23.

Morath, I., Jung, C., Lévêque, R., Linfeng, C., Toillon, R. A., Warth, A., & Orian-Rousseau, V. (2018). Differential recruitment of CD44 isoforms by ErbB ligands reveals an involvement of CD44 in breast cancer. Oncogene, 37(11), 1472-1484.

Klement, J. D., Paschall, A. V., Redd, P. S., Ibrahim, M. L., Lu, C., Yang, D., ... & Liu, K. (2018). An osteopontin/CD44 immune checkpoint controls CD8+ T cell activation and tumor immune evasion. The Journal of clinical investigation, 128(12), 5549-5560.

Suleiman, M., Abdulrahman, N., Yalcin, H., & Mraiche, F. (2018). The role of CD44, hyaluronan and NHE1 in cardiac remodeling. Life sciences, 209, 197-201.

Cortes-Dericks, L., & Schmid, R. A. (2017). CD44 and its ligand hyaluronan as potential biomarkers in malignant pleural mesothelioma: evidence and perspectives. Respiratory research, 18(1), 1-12.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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