Rabbit Anti-CD99 Recombinant Antibody (
9A5) (V2LY-0725-LY648)

Name: Rabbit Anti-CD99 Recombinant Antibody (9A5)
SKU: V2LY-0725-LY648
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-CD99 Recombinant Antibody (9A5) (V2LY-0725-LY648) in WB

Western Blot
Positive WB detected in Jurkat whole cell lysate
All lanes CD99 antibody at 0.8μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 28 KDa
Observed band size: 28 KDa

Rabbit Anti-CD99 Recombinant Antibody (9A5) (V2LY-0725-LY648) in IHC

IHC image of V2LY-0725-LY648 diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Rabbit Anti-CD99 Recombinant Antibody (9A5) (V2LY-0725-LY648) in FC

Overlay histogram showing Jurkat cells stained with V2LY-0725-LY648 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of>10,000 events was performed.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

9A5

Application

ELISA, WB, IHC, FC

Immunogen

A synthesized peptide.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:5,000
IHC	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

CD99 Molecule (Xg Blood Group)

Entrez Gene ID

4267

UniProt ID

P14209

Function

Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes (By similarity).

Biological Process

Homotypic cell-cell adhesion Source: GO_Central
Positive regulation of neutrophil extravasation Source: GO_Central
Regulation of immune response Source: Reactome
T cell extravasation Source: GO_Central

Cellular Location

Membrane

Topology

Extracellular: 23-122
Helical: 123-147
Cytoplasmic: 148-185

PTM

Extensively O-glycosylated.

Siwy, J., Wendt, R., Albalat, A., He, T., Mischak, H., Mullen, W., ... & Beige, J. (2021). CD99 and polymeric immunoglobulin receptor peptides deregulation in critical COVID‐19: A potential link to molecular pathophysiology?. Proteomics, 21(20), 2100133.

Mattisson, J., Danielsson, M., Hammond, M., Davies, H., Gallant, C. J., Nordlund, J., ... & Forsberg, L. A. (2021). Leukocytes with chromosome Y loss have reduced abundance of the cell surface immunoprotein CD99. Scientific Reports, 11(1), 1-9.

Takheaw, N., Sittithumcharee, G., Kariya, R., Kasinrerk, W., & Okada, S. (2021). Anti-human CD99 antibody exerts potent antitumor effects in mantle cell lymphoma. Cancer Immunology, Immunotherapy, 70(6), 1557-1567.

Vaikari, V. P., Park, M., Keossayan, L., MacKay, J. A., & Alachkar, H. (2020). Anti-CD99 scFv-ELP nanoworms for the treatment of acute myeloid leukemia. Nanomedicine: Nanotechnology, Biology and Medicine, 29, 102236.

Wu, J., Zhang, L., Li, H., Wu, S., & Liu, Z. (2019). Nrf2 induced cisplatin resistance in ovarian cancer by promoting CD99 expression. Biochemical and biophysical research communications, 518(4), 698-705.

Cardoso, L. C., Soares, R. D. S., Laurentino, T. D. S., Lerario, A. M., Marie, S. K., & Oba-Shinjo, S. M. (2019). CD99 expression in glioblastoma molecular subtypes and role in migration and invasion. International journal of molecular sciences, 20(5), 1137.

Pasello, M., Manara, M. C., & Scotlandi, K. (2018). CD99 at the crossroads of physiology and pathology. Journal of cell communication and signaling, 12(1), 55-68.

Çelik, H., Sciandra, M., Flashner, B., Gelmez, E., Kayraklıoğlu, N., Allegakoen, D. V., ... & Üren, A. (2018). Clofarabine inhibits Ewing sarcoma growth through a novel molecular mechanism involving direct binding to CD99. Oncogene, 37(16), 2181-2196.

Manara, M. C., Pasello, M., & Scotlandi, K. (2018). CD99: a cell surface protein with an oncojanus role in tumors. Genes, 9(3), 159.

Yeh, C. C., Chang, C. J., Twu, Y. C., Chu, C. C., Liu, B. S., Huang, J. T., ... & Yu, L. C. (2018). The molecular genetic background leading to the formation of the human erythroid-specific Xga/CD99 blood groups. Blood advances, 2(15), 1854-1864.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

CD99 Matched Antibody Pair (241) (CAT#: APMAB-241LY)

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Isotype control

For research use only. Not intended for any clinical use.

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