Mouse Anti-CDAN1 Recombinant Antibody (C0597) (CBMAB-C0597-CQ)

Name: Mouse Anti-CDAN1 Recombinant Antibody (C0597)
SKU: CBMAB-C0597-CQ
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CDAN1 Recombinant Antibody (C0597) (CBMAB-C0597-CQ) in WB

Codanin-1 lengthens C15Orf41 half-life time. Cells were treated with cycloheximide for the indicated times and were run on a western blot. β-tubulin antibody was used as a loading control. Note that the quantity of HA-C15Orf41 DNA in the co-transfection with Codanin-1 was half of the quantity transfected in HA-C15Orf41 and vector treatment in order to get more equal initial levels of HA-C15Orf41. ...View More

Swickley, G., Bloch, Y., Malka, L., Meiri, A., Noy-Lotan, S., Yanai, A., ... & Motro, B. (2020). Characterization of the interactions between Codanin-1 and C15Orf41, two proteins implicated in congenital dyserythropoietic anemia type I disease. BMC Molecular and Cell Biology, 21, 1-12.

Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CDAN1 Recombinant Antibody (C0597) (CBMAB-C0597-CQ) in IF

HeLa cells co-transfected with the indicated constructs were stained with DAPI (blue) and antibodies against HA (green) and Flag (red). The immunofluorescence visualization of the cells was performed with axioimager microscopy. ...View More

Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

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Basic Information

Host Animal

Mouse

Clone

C0597

Application

WB, IF, IP, ELISA

Immunogen

Amino acids 780-1080 mapping near the C-terminus of Codanin-1 of human.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Codanin 1

Introduction

CDAN1 (Codanin 1) is a Protein Coding gene. Diseases associated with CDAN1 include Anemia, Congenital Dyserythropoietic, Type Ia and Congenital Dyserythropoietic Anemia.

Entrez Gene ID

Human	146059
Mouse	68968

UniProt ID

Human	Q8IWY9
Mouse	Q8CC12

Alternative Names

Codanin 1; Congenital Dyserythropoietic Anemia, Type I; Discs Lost Homolog; Codanin-1; PRO1295; CDAN1A; CDA1; CDAI; DLT;

Function

May act as a negative regulator of ASF1 in chromatin assembly.

Biological Process

Chromatin assembly Source: UniProtKB
Chromatin organization Source: MGI
Negative regulation of DNA replication Source: UniProtKB
Protein localization Source: MGI

Cellular Location

Nucleus; Cytoplasm; Membrane. Mainly detected as a cytoplasmic protein.

Involvement in disease

Anemia, congenital dyserythropoietic, 1A (CDAN1A): An autosomal recessive blood disorder characterized by morphological abnormalities of erythroblasts, ineffective erythropoiesis, macrocytic anemia and secondary hemochromatosis. It is occasionally associated with bone abnormalities, especially of the hands and feet (acrodysostosis), nail hypoplasia, and scoliosis. Ultrastructural features include internuclear chromatin bridges connecting some nearly completely separated erythroblasts and an abnormal appearance (spongy or Swiss-cheese appearance) of the heterochromatin in a high proportion of the erythroblasts.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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