Mouse Anti-CDC16 Recombinant Antibody (EG194) (CBMAB-EN208-LY)

Name: Mouse Anti-CDC16 Recombinant Antibody (EG194)
SKU: CBMAB-EN208-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

EG194

Application

WB, IF, IP, ELISA, IHC-P

Immunogen

Amino acids 269-568 mapping at the C-terminus of Cdc16 of human.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Cell Division Cycle 16

Introduction

CDC16 (Cell Division Cycle 16) is a Protein Coding gene. Among its related pathways are PAK Pathway and Actin Nucleation by ARP-WASP Complex.

Entrez Gene ID

Human	8881
Mouse	69957
Rat	290875

UniProt ID

Human	Q13042
Mouse	Q8R349
Rat	Q4V884

Alternative Names

Cell Division Cycle 16; Anaphase-Promoting Complex, Subunit 6; Cyclosome Subunit 6; CDC16Hs; ANAPC6; APC6; CDC16 (Cell Division Cycle 16, S. Cerevisiae, Homolog); CDC16 Cell Division Cycle 16 Homolog (S. Cerevisiae);

Function

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.

Biological Process

Anaphase-promoting complex-dependent catabolic process Source: GO_Central
Cell division Source: GO_Central
Metaphase/anaphase transition of mitotic cell cycle Source: GO_Central
Positive regulation of mitotic metaphase/anaphase transition Source: GO_Central
Protein K11-linked ubiquitination Source: UniProtKB
Protein ubiquitination Source: GO_Central
Regulation of exit from mitosis Source: Reactome
Regulation of mitotic cell cycle phase transition Source: Reactome
Ubiquitin-dependent protein catabolic process Source: Reactome

Cellular Location

Centrosome; Spindle. Colocalizes with CDC27 to the centrosome at all stages of the cell cycle and to the mitotic spindle.

PTM

Phosphorylated. Phosphorylation on Ser-560 ^oCcurs specifically during mitosis.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-CDC16 Recombinant Antibody (0446) (CAT#: PTM-CBPAB-0446YC)

Rabbit Anti-CDC16 Recombinant Antibody (EG554) (CAT#: CBMAB-EN669-LY)

Mouse Anti-CDC16 Recombinant Antibody (0445) (CAT#: PTM-CBPAB-0445YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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