Rabbit Anti-CDC25C Recombinant Antibody (
3E6) (V2LY-0725-LY650)

Name: Rabbit Anti-CDC25C Recombinant Antibody (3E6)
SKU: V2LY-0725-LY650
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-CDC25C Recombinant Antibody (3E6) (V2LY-0725-LY650) in WB

Western Blot
Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, Raji whole cell lysate, 293 whole cell lysate
All lanes: Cdc25C antibody at 1.65μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 54, 52, 49, 46 KDa
Observed band size: 60 KDa

Rabbit Anti-CDC25C Recombinant Antibody (3E6) (V2LY-0725-LY650) in IHC

IHC image of V2LY-0725-LY650 diluted at 1:165 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Rabbit Anti-CDC25C Recombinant Antibody (3E6) (V2LY-0725-LY650) in IF

Immunofluorescence staining of Hela cells with V2LY-0725-LY650 at 1:55, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Rabbit Anti-CDC25C Recombinant Antibody (3E6) (V2LY-0725-LY650) in IP

Immunoprecipitating CDC25C in HEK293 whole cell lysate
Lane 1: Rabbit control IgG instead of V2LY-0725-LY650 in HEK293 whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: V2LY-0725-LY650 (3μg) + HEK293 whole cell lysate (500μg)
Lane 3: HEK293 whole cell lysate (20μg)

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

3E6

Application

ELISA, WB, IHC, IF, IP

Immunogen

A synthesized peptide derived from human CDC25C.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:5,000
IHC	1:50-1:200
IF	1:20-1:200
IP	1:200-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Cell Division Cycle 25C

Entrez Gene ID

995

UniProt ID

P30307

Function

Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity.

Biological Process

Cell division Source: UniProtKB-KW
Cell population proliferation Source: ProtInc
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest Source: Reactome
G2/M transition of mitotic cell cycle Source: UniProtKB
Positive regulation of G2/MI transition of meiotic cell cycle Source: GO_Central
Positive regulation of G2/M transition of mitotic cell cycle Source: GO_Central
Regulation of cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB
Regulation of mitotic nuclear division Source: ProtInc
Spermatogenesis Source: Ensembl
Viral process Source: UniProtKB-KW

Cellular Location

Nucleus

PTM

Phosphorylated by CHEK1 and MAPK14 at Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4. Phosphorylated by PLK1, leading to activate the phosphatase activity. Phosphorylation by PLK3 at Ser-191 promotes nuclear translocation. Ser-198 is a minor phosphorylation site. Was initially reported to be phosphorylated by PLK3 at Ser-216 (PubMed:10557092). However, such phosphorylation by PLK3 was not confirmed by other groups. Phosphorylation at Thr-48, Thr-67, Ser-122, Thr-130, Ser-168 and Ser-214 ^oCcurs at G2 and G2-M transition and is probably catalyzed by CDK1. Ser-168 phosphorylation levels are lower than those at the other 5 CDK1 sites. Phosphorylation by CDK1 leads to increased activity.

Liu, K., Zheng, M., Lu, R., Du, J., Zhao, Q., Li, Z., ... & Zhang, S. (2020). The role of CDC25C in cell cycle regulation and clinical cancer therapy: a systematic review. Cancer Cell International, 20, 1-16.

Galusic, D., Lucijanic, M., Livun, A., Radman, M., Lucijanic, J., Hofman, I. D., & Kusec, R. (2020). CDC25c expression in patients with myelofibrosis is associated with stronger myeloproliferation and shorter overall survival. Wiener klinische Wochenschrift, 1-3.

Xun, R., Lu, H., & Wang, X. (2020). Identification of CDC25C as a Potential Biomarker in Hepatocellular Carcinoma Using Bioinformatics Analysis. Technology in Cancer Research & Treatment, 19, 1533033820967474.

Xia, Z., Ou-Yang, W., Hu, T., & Du, K. (2019). Prognostic significance of CDC25C in lung adenocarcinoma: an analysis of TCGA data. Cancer genetics, 233, 67-74.

Li, H., Liang, D., Hu, N., Dai, X., He, J., Zhuang, H., & Zhao, W. (2019). Helicobacter pylori inhibited cell proliferation in human periodontal ligament fibroblasts through the Cdc25C/CDK1/cyclinB1 signaling cascade. Journal of periodontal & implant science, 49(3), 138-147.

Jayasooriya, R. G. P. T., Dilshara, M. G., Molagoda, I. M. N., Park, C., Park, S. R., Lee, S., ... & Kim, G. Y. (2018). Camptothecin induces G2/M phase arrest through the ATM-Chk2-Cdc25C axis as a result of autophagy-induced cytoprotection: Implications of reactive oxygen species. Oncotarget, 9(31), 21744.

Lal, N., Nemaysh, V., & Luthra, P. M. (2018). Proteasome mediated degradation of CDC25C and Cyclin B1 in Demethoxycurcumin treated human glioma U87 MG cells to trigger G2/M cell cycle arrest. Toxicology and applied pharmacology, 356, 76-89.

Ojiegbe, S., Joseph, C., Provenzano, E., Caldas, C., Nolan, C., Green, A. R., ... & Mukherjee, A. (2017). Cell division cycle 25C (CDC25C) expression confers poor prognosis in invasive breast cancer.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

CDC25C Matched Antibody Pair (245) (CAT#: APMAB-245LY)

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CDC25C Matched Antibody Pair (245) (CAT#: APMAB-245LY)

Isotype control

For research use only. Not intended for any clinical use.

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