Rabbit Anti-CDK12 Recombinant Antibody (CBYY-C1755) (CBMAB-C3193-YY)

Name: Rabbit Anti-CDK12 Recombinant Antibody (CBYY-C1755)
SKU: CBMAB-C3193-YY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

CBYY-C1755

Application

WB, IP

Immunogen

Synthetic peptide corresponding to residues surrounding Ala497 of human CDK12 protein.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:1,000
IP	1:50

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

HEPES, pH 7.5, 150 mM NaCl, 100 µg/ml BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

cyclin-dependent kinase 12

Entrez Gene ID

51755

UniProt ID

Q9NYV4

Alternative Names

Cyclin Dependent Kinase 7; Cyclin-Dependent Kinase 7 (MO15 Homolog, Xenopus Laevis, Cdk-Activating Kinase); TFIIH Basal Transcription Factor Complex Kinase Subunit; Cell Division Protein Kinase 7; CDK-Activating Kinase 1; 39 KDa Protein Kinase; EC 2.7.11.22; CDKN7; STK1; CAK1; MO15; CAK; Cyclin-Dependent Kinase 7 (Homolog Of Xenopus MO15 Cdk-Activating Kinase); Homolog Of Xenopus MO15 Cdk-Activating Kinase;

Function

Cyclin-dependent kinase that phosphorylates the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A), thereby acting as a key regulator of transcription elongation. Regulates the expression of genes involved in DNA repair and is required for the maintenance of genomic stability. Preferentially phosphorylates 'Ser-5' in CTD repeats that are already phosphorylated at 'Ser-7', but can also phosphorylate 'Ser-2'. Required for RNA splicing, possibly by phosphorylating SRSF1/SF2. Involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogen inhibitors.

Biological Process

mRNA processing Source: UniProtKB-KW
Negative regulation of stem cell differentiation Source: Ensembl
Phosphorylation of RNA polymerase II C-terminal domain Source: UniProtKB
Positive regulation of transcription elongation from RNA polymerase II promoter Source: GO_Central
Protein autophosphorylation Source: HGNC-UCL
Protein phosphorylation Source: GO_Central
Regulation of MAP kinase activity Source: UniProtKB
Regulation of RNA splicing Source: Ensembl
RNA splicing Source: UniProtKB
Transcription elongation from RNA polymerase II promoter Source: GO_Central

Cellular Location

Nucleus; Nucleus speckle. Colocalized with nuclear speckles throughout interphase.

Involvement in disease

Chromosomal aberrations involving CDK12 may be a cause gastric cancer. Deletions within 17q12 region producing fusion transcripts with ERBB2, leading to CDK12-ERBB2 fusion leading to trunctated CDK12 protein not in-frame with ERBB2.

PTM

Phosphorylation at Thr-893 increases kinase activity.

Liu, H., Liu, K., & Dong, Z. (2021). Targeting CDK12 for cancer therapy: function, mechanism, and drug discovery. Cancer research, 81(1), 18-26.

Rescigno, P., Gurel, B., Pereira, R., Crespo, M., Rekowski, J., Rediti, M., ... & de Bono, J. S. (2021). Characterizing CDK12-mutated prostate cancers. Clinical Cancer Research, 27(2), 566-574.

Reimers, M. A., Yip, S. M., Zhang, L., Cieslik, M., Dhawan, M., Montgomery, B., ... & Chou, J. (2020). Clinical outcomes in cyclin-dependent kinase 12 mutant advanced prostate cancer. European urology, 77(3), 333-341.

Liu, Y., Hao, M., Leggett, A. L., Gao, Y., Ficarro, S. B., Che, J., ... & Gray, N. S. (2020). Discovery of MFH290: a potent and highly selective covalent inhibitor for cyclin-dependent kinase 12/13. Journal of Medicinal Chemistry, 63(13), 6708-6726.

Thanindratarn, P., Dean, D. C., Feng, W., Wei, R., Nelson, S. D., Hornicek, F. J., & Duan, Z. (2020). Cyclin-dependent kinase 12 (CDK12) in chordoma: prognostic and therapeutic value. European Spine Journal, 29(12), 3214-3228.

Liang, S., Hu, L., Wu, Z., Chen, Z., Liu, S., Xu, X., & Qian, A. (2020). CDK12: A potent target and biomarker for human cancer therapy. Cells, 9(6), 1483.

Emadi, F., Teo, T., Rahaman, M. H., & Wang, S. (2020). CDK12: a potential therapeutic target in cancer. Drug Discovery Today.

Antonarakis, E. S. (2018). Cyclin-dependent kinase 12, immunity, and prostate cancer. New England Journal of Medicine, 379(11), 1087-1089.

Lui, G. Y., Grandori, C., & Kemp, C. J. (2018). CDK12: an emerging therapeutic target for cancer. Journal of clinical pathology, 71(11), 957-962.

Johannes, J. W., Denz, C. R., Su, N., Wu, A., Impastato, A. C., Mlynarski, S., ... & Guichard, S. (2018). Structure‐Based Design of Selective Noncovalent CDK12 Inhibitors. ChemMedChem, 13(3), 231-235.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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