Human Recombinant CDK12 protein, GST Tag (V2LY-0526-LY3022)

Name: Human Recombinant CDK12 protein, GST Tag
SKU: V2LY-0526-LY3022
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

GST Tag

Protein Construction

This product is Human Recombinant CDK12 protein, GST Tag consist of Amino Acid: 658-end and predicts a molecular mass of 135 kDa.

Molecule Mass

135 kDa

Sequence

Amino Acid: 658-end

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

Batch dependent.

Endotoxin

Please contact us for more information.

Format

Liquid

Buffer

Please contact us for more information.

Preservative

None

Storage

Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

More Infomation

Target

Full Name

cyclin-dependent kinase 12

Function

Cyclin-dependent kinase that phosphorylates the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A), thereby acting as a key regulator of transcription elongation. Regulates the expression of genes involved in DNA repair and is required for the maintenance of genomic stability. Preferentially phosphorylates 'Ser-5' in CTD repeats that are already phosphorylated at 'Ser-7', but can also phosphorylate 'Ser-2'. Required for RNA splicing, possibly by phosphorylating SRSF1/SF2. Involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogen inhibitors.

Biological Process

mRNA processing Source: UniProtKB-KW
Negative regulation of stem cell differentiation Source: Ensembl
Phosphorylation of RNA polymerase II C-terminal domain Source: UniProtKB
Positive regulation of transcription elongation from RNA polymerase II promoter Source: GO_Central
Protein autophosphorylation Source: HGNC-UCL
Protein phosphorylation Source: GO_Central
Regulation of MAP kinase activity Source: UniProtKB
Regulation of RNA splicing Source: Ensembl
RNA splicing Source: UniProtKB
Transcription elongation from RNA polymerase II promoter Source: GO_Central

Cellular Location

Nucleus; Nucleus speckle. Colocalized with nuclear speckles throughout interphase.

Involvement in disease

Chromosomal aberrations involving CDK12 may be a cause gastric cancer. Deletions within 17q12 region producing fusion transcripts with ERBB2, leading to CDK12-ERBB2 fusion leading to trunctated CDK12 protein not in-frame with ERBB2.

PTM

Phosphorylation at Thr-893 increases kinase activity.

Liu, H., Liu, K., & Dong, Z. (2021). Targeting CDK12 for cancer therapy: function, mechanism, and drug discovery. Cancer research, 81(1), 18-26.

Rescigno, P., Gurel, B., Pereira, R., Crespo, M., Rekowski, J., Rediti, M., ... & de Bono, J. S. (2021). Characterizing CDK12-mutated prostate cancers. Clinical Cancer Research, 27(2), 566-574.

Reimers, M. A., Yip, S. M., Zhang, L., Cieslik, M., Dhawan, M., Montgomery, B., ... & Chou, J. (2020). Clinical outcomes in cyclin-dependent kinase 12 mutant advanced prostate cancer. European urology, 77(3), 333-341.

Liu, Y., Hao, M., Leggett, A. L., Gao, Y., Ficarro, S. B., Che, J., ... & Gray, N. S. (2020). Discovery of MFH290: a potent and highly selective covalent inhibitor for cyclin-dependent kinase 12/13. Journal of Medicinal Chemistry, 63(13), 6708-6726.

Thanindratarn, P., Dean, D. C., Feng, W., Wei, R., Nelson, S. D., Hornicek, F. J., & Duan, Z. (2020). Cyclin-dependent kinase 12 (CDK12) in chordoma: prognostic and therapeutic value. European Spine Journal, 29(12), 3214-3228.

Liang, S., Hu, L., Wu, Z., Chen, Z., Liu, S., Xu, X., & Qian, A. (2020). CDK12: A potent target and biomarker for human cancer therapy. Cells, 9(6), 1483.

Emadi, F., Teo, T., Rahaman, M. H., & Wang, S. (2020). CDK12: a potential therapeutic target in cancer. Drug Discovery Today.

Antonarakis, E. S. (2018). Cyclin-dependent kinase 12, immunity, and prostate cancer. New England Journal of Medicine, 379(11), 1087-1089.

Lui, G. Y., Grandori, C., & Kemp, C. J. (2018). CDK12: an emerging therapeutic target for cancer. Journal of clinical pathology, 71(11), 957-962.

Johannes, J. W., Denz, C. R., Su, N., Wu, A., Impastato, A. C., Mlynarski, S., ... & Guichard, S. (2018). Structure‐Based Design of Selective Noncovalent CDK12 Inhibitors. ChemMedChem, 13(3), 231-235.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant CDK12 & CCNK, Active protein, GST Tag (CAT#: V2LY-0526-LY3021)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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