Mouse Anti-CDK5R2 Recombinant Antibody (CBYC-P044) (CBMAB-P0175-YC)

Name: Mouse Anti-CDK5R2 Recombinant Antibody (CBYC-P044)
SKU: CBMAB-P0175-YC
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CDK5R2 Recombinant Antibody (CBYC-P044) (CBMAB-P0175-YC) in WB

Top, representative blots of CDK5, p35, p39 with their respective GAPDH. Bottom, quantification of CDK5, p35 and p39 fold change (U18/CTL) normalized to GAPDH. N = 4 (CDK5), n = 3 (p35), and n = 3 (p39) across independent isolations. ...View More

Casas, M., Murray, K. D., Hino, K., Vierra, N. C., Simó, S., Trimmer, J. S., ... & Dickson, E. J. (2023). NPC1-dependent alterations in KV2. 1-CaV1. 2 nanodomains drive neuronal death in models of Niemann-Pick Type C disease. Nature Communications, 14(1), 4553.

Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CDK5R2 Recombinant Antibody (CBYC-P044) (CBMAB-P0175-YC) in WB

Developmental expression of WDFY1 in rat cortical neurons at 4, 7, 10 and 14 days in vitro (DIV). ...View More

Ouyang, L., Chen, Y., Wang, Y., Chen, Y., Fu, A. K., Fu, W. Y., & Ip, N. Y. (2020). p39-associated Cdk5 activity regulates dendritic morphogenesis. Scientific reports, 10(1), 18746.

Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CDK5R2 Recombinant Antibody (CBYC-P044) (CBMAB-P0175-YC) in IF

Immunofluorescence of p39 (red) in the corpus callosum of normal (a), 3 weeks DM (b), and 3 + 3 weeks RM (c and d) mice. DAPI staining (blue) marks nuclei. ...View More

Bankston, A. N., Li, W., Zhang, H., Ku, L., Liu, G., Papa, F., ... & Feng, Y. (2013). p39, the primary activator for cyclin-dependent kinase 5 (Cdk5) in oligodendroglia, is essential for oligodendroglia differentiation and myelin repair. Journal of Biological Chemistry, 288(25), 18047-18057.

Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYC-P044

Application

ELISA, WB, IP, IF

Immunogen

Amino acids 6-75 mapping near the N-terminus of p39 of human.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Cyclin Dependent Kinase 5 Regulatory Subunit 2

Introduction

CDK5R2 (Cyclin Dependent Kinase 5 Regulatory Subunit 2) is a protein coding gene. Among its related pathways are Lissencephaly gene (LIS1) in neuronal migration and development and Reelin Pathway (Cajal-Retzius cells). Gene Ontology annotations related to this gene include lipid binding and cyclin-dependent protein kinase 5 activator activity. An important paralog of the gene is CDK5R1.

Entrez Gene ID

Human	8941
Mouse	12570
Rat	501164

UniProt ID

Human	Q13319
Mouse	O35926
Rat	F1M491

Alternative Names

p39nck5ai; P39; NCK5AI

Function

Activator of CDK5/TPKII.

Biological Process

Cerebellum development Source: Ensembl
Hippocampus development Source: Ensembl
Layer formation in cerebral cortex Source: Ensembl
Neuron migration Source: Ensembl
Positive regulation of calcium ion-dependent exocytosis Source: Ensembl
Regulation of cyclin-dependent protein serine/threonine kinase activity Source: ProtInc
Superior olivary nucleus maturation Source: Ensembl

Cellular Location

Cell membrane

PTM

Myristoylated. The Gly-2-Ala mutant is absent of the cell periphery, suggesting that a proper myristoylation signal is essential for the proper distribution of CDK5R2 (p39).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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