Mouse Anti-CEBPD Recombinant Antibody (CBXC-1705) (CBMAB-C3007-CQ)

Name: Mouse Anti-CEBPD Recombinant Antibody (CBXC-1705)
SKU: CBMAB-C3007-CQ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBXC-1705

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 252-269 at the C-terminus of C/EBP δ of human.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

CCAAT/Enhancer Binding Protein Delta

Introduction

The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related protein CEBP-alpha. The encoded protein is important in the regulation of genes involved in immune and inflammatory responses, and may be involved in the regulation of genes associated with activation and/or differentiation of macrophages. The cytogenetic location of this locus has been reported as both 8p11 and 8q11.

Entrez Gene ID

12609

UniProt ID

Q00322

Alternative Names

CCAAT/Enhancer Binding Protein Delta; CCAAT/Enhancer Binding Protein (C/EBP), Delta; Nuclear Factor NF-IL6-Beta; C/EBP Delta; NF-IL6-Beta; CCAAT/Enhancer-Binding Protein Delta; C/EBP-Delta; CELF; CRP3;

Function

Transcription activator that recognizes two different DNA motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers (PubMed:16397300).
Important transcription factor regulating the expression of genes involved in immune and inflammatory responses (PubMed:1741402, PubMed:16397300).
Transcriptional activator that enhances IL6 transcription alone and as heterodimer with CEBPB (PubMed:1741402).

Biological Process

Cytokine-mediated signaling pathway Source: Reactome
Regulation of cell differentiation Source: GO_Central
Regulation of transcription by RNA polymerase II Source: GO_Central
Transcription by RNA polymerase II Source: ProtInc
Viral life cycle Source: Reactome

Cellular Location

Nucleus

Wang, S. M., Lin, W. C., Lin, H. Y., Chen, Y. L., Ko, C. Y., & Wang, J. M. (2021). CCAAT/Enhancer-binding protein delta mediates glioma stem-like cell enrichment and ATP-binding cassette transporter ABCA1 activation for temozolomide resistance in glioblastoma. Cell Death Discovery, 7(1), 1-11.

Liu, L., Zhong, J., Chen, B., Wang, W., Xi, H., & Su, X. (2021). CCAAT/enhancer binding protein (C/EBP) delta promotes the expression of PTX3 and macrophage phagocytosis during A. fumigatus infection. Journal of Leukocyte Biology.

Duitman, J., Hartl, L., Roelofs, J. J., Bijlsma, M. F., & Spek, C. A. (2021). Non-Tumor CCAAT/Enhancer-Binding Protein Delta Potentiates Tumor Cell Extravasation and Pancreatic Cancer Metastasis Formation. Biomolecules, 11(8), 1079.

Spek, C. A., Aberson, H. L., Butler, J. M., de Vos, A. F., & Duitman, J. (2021). CEBPD Potentiates the Macrophage Inflammatory Response but CEBPD Knock-Out Macrophages Fail to Identify CEBPD-Dependent Pro-Inflammatory Transcriptional Programs. Cells, 10(9), 2233.

Wang, S. M., Lin, H. Y., Chen, Y. L., Hsu, T. I., Chuang, J. Y., Kao, T. J., & Ko, C. Y. (2019). CCAAT/enhancer-binding protein delta regulates the stemness of glioma stem-like cells through activating PDGFA expression upon inflammatory stimulation. Journal of neuroinflammation, 16(1), 1-12.

Liu, P., Cao, W., Ma, B., Li, M., Chen, K., Sideras, K., ... & Peppelenbosch, M. P. (2019). Action and clinical significance of CCAAT/enhancer-binding protein delta in hepatocellular carcinoma. Carcinogenesis, 40(1), 155-163.

Wang, S. M., Lim, S. W., Wang, Y. H., Lin, H. Y., Lai, M. D., Ko, C. Y., & Wang, J. M. (2018). Astrocytic CCAAT/Enhancer-binding protein delta contributes to reactive oxygen species formation in neuroinflammation. Redox biology, 16, 104-112.

Tsai, H. H., Lai, H. Y., Chen, Y. C., Li, C. F., Huang, H. S., Liu, H. S., ... & Wang, J. M. (2017). Metformin promotes apoptosis in hepatocellular carcinoma through the CEBPD-induced autophagy pathway. Oncotarget, 8(8), 13832.

Lai, H. Y., Hsu, L. W., Tsai, H. H., Lo, Y. C., Yang, S. H., Liu, P. Y., & Wang, J. M. (2017). CCAAT/enhancer-binding protein delta promotes intracellular lipid accumulation in M1 macrophages of vascular lesions. Cardiovascular research, 113(11), 1376-1388.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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