Mouse Anti-CELA1 Recombinant Antibody (4H5) (CBMAB-C7062-LY)

Name: Mouse Anti-CELA1 Recombinant Antibody (4H5)
SKU: CBMAB-C7062-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

4H5

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

Recombinant protein representing full-length Elastase-1 of human.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.1 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Chymotrypsin Like Elastase Family Member 1

Introduction

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, pancreatic elastase 1 is not expressed in the pancreas. To date, elastase 1 expression has only been detected in skin keratinocytes. Clinical literature that describes human elastase 1 activity in the pancreas or fecal material is actually referring to chymotrypsin-like elastase family, member 3B. [provided by RefSeq, May 2009]

Entrez Gene ID

1990

UniProt ID

Q9UNI1

Alternative Names

Chymotrypsin Like Elastase Family Member 1; Elastase 1, Pancreatic; Pancreatic Elastase 1; EC 3.4.21.36; Elastase-1; ELA1; Chymotrypsin-Like Elastase Family, Member 1; Chymotrypsin-Like Elastase Family Member 1; EC 3.4.21;

Function

Acts upon elastin.

Biological Process

Elastin catabolic process Source: Ensembl
Exocrine pancreas development Source: Ensembl
Inflammatory response Source: Ensembl
Multicellular organism growth Source: Ensembl
Negative regulation of transcription by RNA polymerase II Source: Ensembl
Pancreas morphogenesis Source: Ensembl
Positive regulation of angiogenesis Source: Ensembl
Positive regulation of transcription by RNA polymerase II Source: Ensembl
Post-embryonic development Source: Ensembl
Proteolysis Source: GO_Central
Regulation of cell differentiation Source: Ensembl
Regulation of cell population proliferation Source: Ensembl
Tissue remodeling Source: Ensembl
Wnt signaling pathway Source: Ensembl

Cellular Location

Secreted

Ojha, M., Joshi, R., Goodman, E., Lewis, J., Fan, Q., Schumann, R., ... & Varisco, B. (2021). Chymotrypsin-like Elastase 1 is Required for Emphysema Progression. medRxiv, 2020-04.

Ojha, M., Joshi, R., Lewis, J., Qiang, F., & Varisco, B. M. (2019). Chymotrypsin-Like Elastase 1 Is Required for Late Lung Remodeling in Mouse Porcine Pancreatic Elastase Model of Emphysema. In C63. MECHANISTIC STUDIES IN COPD (pp. A5365-A5365). American Thoracic Society.

Joshi, R., Heinz, A., Fan, Q., Guo, S., Monia, B., Schmelzer, C. E., ... & Varisco, B. M. (2018). Role for cela1 in postnatal lung remodeling and alpha-1 antitrypsin–deficient emphysema. American journal of respiratory cell and molecular biology, 59(2), 167-178.

Joshi, R., Heinz, A., Fan, Q., Guo, S., Monia, B., Schmelzer, C. E., ... & Varisco, B. M. (2017). Role of Chymotrypsin-like Elastase 1 in Lung Physiology and in α1-Antitrypsin Deficiency. bioRxiv, 138776.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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