Human CELA1 ELISA Kit (V2LY-0626-LY4411)

Name: Human CELA1 ELISA Kit
SKU: V2LY-0626-LY4411
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.17 ng/mL

Detection Range

0.3-90 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

27.8 kDa

Components

Pre-coated ELISA plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

Chymotrypsin Like Elastase Family Member 1

Function

Acts upon elastin.

Biological Process

Elastin catabolic process Source: Ensembl
Exocrine pancreas development Source: Ensembl
Inflammatory response Source: Ensembl
Multicellular organism growth Source: Ensembl
Negative regulation of transcription by RNA polymerase II Source: Ensembl
Pancreas morphogenesis Source: Ensembl
Positive regulation of angiogenesis Source: Ensembl
Positive regulation of transcription by RNA polymerase II Source: Ensembl
Post-embryonic development Source: Ensembl
Proteolysis Source: GO_Central
Regulation of cell differentiation Source: Ensembl
Regulation of cell population proliferation Source: Ensembl
Tissue remodeling Source: Ensembl
Wnt signaling pathway Source: Ensembl

Cellular Location

Secreted

Ojha, M., Joshi, R., Goodman, E., Lewis, J., Fan, Q., Schumann, R., ... & Varisco, B. (2021). Chymotrypsin-like Elastase 1 is Required for Emphysema Progression. medRxiv, 2020-04.

Ojha, M., Joshi, R., Lewis, J., Qiang, F., & Varisco, B. M. (2019). Chymotrypsin-Like Elastase 1 Is Required for Late Lung Remodeling in Mouse Porcine Pancreatic Elastase Model of Emphysema. In C63. MECHANISTIC STUDIES IN COPD (pp. A5365-A5365). American Thoracic Society.

Joshi, R., Heinz, A., Fan, Q., Guo, S., Monia, B., Schmelzer, C. E., ... & Varisco, B. M. (2018). Role for cela1 in postnatal lung remodeling and alpha-1 antitrypsin–deficient emphysema. American journal of respiratory cell and molecular biology, 59(2), 167-178.

Joshi, R., Heinz, A., Fan, Q., Guo, S., Monia, B., Schmelzer, C. E., ... & Varisco, B. M. (2017). Role of Chymotrypsin-like Elastase 1 in Lung Physiology and in α1-Antitrypsin Deficiency. bioRxiv, 138776.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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