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Mouse Anti-CERS2 Recombinant Antibody (CBYJL-090) (CBMAB-L0146-YJ)

Provided herein is a Mouse monoclonal antibody, which binds to Ceramide Synthase 2 (CERS2). The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all CERS2 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYJL-090
Antibody Isotype
IgG2a, κ
Application
ELISA, WB

Basic Information

Immunogen
LASS2 (AAH01357, 1aa-380aa) full-length recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH 7.4
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 1-380

Target

Full Name
Ceramide Synthase 2
Introduction
CERS2 has sequence similarity to yeast longevity assurance gene 1. Mutation or overexpression of the related gene in yeast has been shown to alter yeast lifespan. The human protein may functs in the regulation of cell growth.
Entrez Gene ID
UniProt ID
Alternative Names
Ceramide Synthase 2; Longevity Assurance (LAG1; S. Cerevisiae) Homolog 2; Tumor Metastasis-Suppressor Gene 1 Protein; LAG1 Homolog; Ceramide Synthase 2; LASS2; SP260; TMSG1
Function
Ceramide synthase that catalyzes formation of ceramide from sphinganine and acyl-CoA substrates, with high selectivity toward very-long (C22:0-C24:0) chain as acyl donor. May regulate lipid metabolism in hepatocytes (By similarity).
Biological Process
Ceramide biosynthetic process Source: UniProtKB
Negative regulation of axon regeneration Source: BHF-UCL
Negative regulation of Schwann cell migration Source: BHF-UCL
Negative regulation of Schwann cell proliferation involved in axon regeneration Source: BHF-UCL
Regulation of lipid metabolic process Source: UniProtKB
Sphingolipid biosynthetic process Source: Reactome
Cellular Location
Endoplasmic reticulum membrane
Topology
Lumenal: 1-40
Helical: 41-61
Helical: 140-160
Helical: 181-201
Helical: 209-229
Helical: 264-284
Helical: 304-324
Cytoplasmic: 325-380
PTM
Acetylated. Deacetylation by SIRT3 increases enzyme activity and promotes mitochondrial ceramide accumulation.
Phosphorylated at the C-terminus by CK2, leading to increase the ceramide synthase activity.

Barklis, E., Alfadhli, A., Kyle, J. E., Bramer, L. M., Bloodsworth, K. J., Barklis, R. L., ... & Tafesse, F. G. (2021). Ceramide synthase 2 deletion decreases the infectivity of HIV-1. Journal of Biological Chemistry, 296.

Zhang, X., Sakamoto, W., Canals, D., Ishibashi, M., Matsuda, M., Nishida, K., ... & Kitatani, K. (2021). Ceramide synthase 2‐C24: 1‐ceramide axis limits the metastatic potential of ovarian cancer cells. The FASEB Journal, 35(2), e21287.

Pani, T., Rajput, K., Kar, A., Sharma, H., Basak, R., Medatwal, N., ... & Dasgupta, U. (2021). Alternative splicing of ceramide synthase 2 alters levels of specific ceramides and modulates cancer cell proliferation and migration in Luminal B breast cancer subtype. Cell death & disease, 12(2), 1-22.

Shin, S. H., Cho, K. A., Yoon, H. S., Kim, S. Y., Kim, H. Y., Pewzner-Jung, Y., ... & Park, J. W. (2021). Ceramide Synthase 2 Null Mice Are Protected from Ovalbumin-Induced Asthma with Higher T Cell Receptor Signal Strength in CD4+ T Cells. International journal of molecular sciences, 22(5), 2713.

Zhang, Q., Wang, J. Y., Yan, W., Wang, D. D., Yang, S. J., Zhou, S. Y., ... & Tang, J. H. (2019). Clinical and pathological significance of Homo sapiens ceramide synthase 2 (CerS-2) in diverse human cancers. Bioscience reports, 39(5), BSR20181743.

Bickert, A., Kern, P., van Uelft, M., Herresthal, S., Ulas, T., Gutbrod, K., ... & Willecke, K. (2018). Inactivation of ceramide synthase 2 catalytic activity in mice affects transcription of genes involved in lipid metabolism and cell division. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids, 1863(7), 734-749.

Kim, Y. R., Volpert, G., Shin, K. O., Kim, S. Y., Shin, S. H., Lee, Y., ... & Park, J. W. (2017). Ablation of ceramide synthase 2 exacerbates dextran sodium sulphate‐induced colitis in mice due to increased intestinal permeability. Journal of cellular and molecular medicine, 21(12), 3565-3578.

Oertel, S., Scholich, K., Weigert, A., Thomas, D., Schmetzer, J., Trautmann, S., ... & Grösch, S. (2017). Ceramide synthase 2 deficiency aggravates AOM-DSS-induced colitis in mice: role of colon barrier integrity. Cellular and Molecular Life Sciences, 74(16), 3039-3055.

Rieck, M., Kremser, C., Jobin, K., Mettke, E., Kurts, C., Gräler, M., ... & Kolanus, W. (2017). Ceramide synthase 2 facilitates S1P‐dependent egress of thymocytes into the circulation in mice. European journal of immunology, 47(4), 677-684.

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For research use only. Not intended for any clinical use.

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