Mouse Anti-CERS4 Recombinant Antibody (CBYJL-099) (CBMAB-L0155-YJ)

Provided herein is a Mouse monoclonal antibody, which binds to Ceramide Synthase 4 (CERS4). The antibody can be used for immunoassay techniques, such as ELISA, IF, WB.
See all CERS4 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBYJL-099

Antibody Isotype

IgG2a, κ

Application

ELISA, IF, WB

Basic Information

Immunogen

Partial recombinant corresponding to aa57-140 from human LASS4 (NP_078828) with GST tag. MW of the GST tag alone is 26kD.

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, pH 7.4

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 57-140

Target

Full Name

Ceramide Synthase 4

Introduction

Among CERS4's related pathways are Metabolism and Sphingolipid metabolism. Gene Ontology (GO) annotations related to this gene include sphingosine N-acyltransferase activity. An important paralog of this gene is CERS2.

Entrez Gene ID

79603

UniProt ID

Q9HA82

Alternative Names

Ceramide Synthase 4; LAG1 Longevity Assurance Homolog 4; LAG1 Homolog; Ceramide Synthase 4; LASS4; LAG1 Longevity Assurance Homolog 4 (S. Cerevisiae); CerS4; Trh1

Function

Ceramide synthase that catalyzes formation of ceramide from sphinganine and acyl-CoA substrates, with high selectivity toward long and very-long chains (C18:0-C22:0) as acyl donor.

Biological Process

Ceramide biosynthetic process Source: UniProtKB
Sphingolipid biosynthetic process Source: Reactome

Cellular Location

Endoplasmic reticulum membrane

Topology

Lumenal: 1-31
Helical: 32-52
Helical: 140-160
Helical: 179-199
Helical: 209-229
Helical: 260-280
Helical: 304-324
Cytoplasmic: 325-394

PTM

Phosphorylated at the C-terminus by CK2.
N-glycosylated.

References

Chen, J., Li, X., Ma, D., Liu, T., Tian, P., & Wu, C. (2021). Ceramide synthase-4 orchestrates the cell proliferation and tumor growth of liver cancer in vitro and in vivo through the nuclear factor-κB signaling pathway. Oncology Letters, 21(4).

Peters, F., Tellkamp, F., Brodesser, S., Wachsmuth, E., Tosetti, B., Karow, U., ... & Niessen, C. M. (2020). Murine Epidermal Ceramide Synthase 4 Is a Key Regulator of Skin Barrier Homeostasis. Journal of Investigative Dermatology, 140(10), 1927-1937.

Dix, F. L. (2018). Ceramide synthase 4: a novel metabolic regulator of oncogene-induced senescence (Doctoral dissertation, University of Edinburgh).

Ito, S., Ishikawa, J., Naoe, A., Yoshida, H., Hachiya, A., Fujimura, T., ... & Takema, Y. (2017). Ceramide synthase 4 is highly expressed in involved skin of patients with atopic dermatitis. Journal of the European Academy of Dermatology and Venereology, 31(1), 135-141.

Chen, J., Li, X., Ma, D., Liu, T., Tian, P., & Wu, C. (2017). Ceramide synthase-4 orchestrates the cell proliferation and tumor growth of liver cancer in vitro and in vivo through the nuclear factor-κB signaling pathway. Oncology letters, 14(2), 1477-1483.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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