Mouse Anti-CHAC1 Recombinant Antibody (1G1) (CBMAB-C4113-LY)

Name: Mouse Anti-CHAC1 Recombinant Antibody (1G1)
SKU: CBMAB-C4113-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

1G1

Application

IF, WB

Immunogen

Full length human recombinant protein of human CHAC1.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IF(ICC)	1:100
WB	1:4,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 1% BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

1 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

ChaC Glutathione Specific Gamma-Glutamylcyclotransferase 1

Introduction

This gene encodes a member of the gamma-glutamylcyclotransferase family of proteins. The encoded protein has been shown to promote neuronal differentiation by deglycination of the Notch receptor, which prevents receptor maturation and inhibits Notch signaling. This protein may also play a role in the unfolded protein response, and in regulation of glutathione levels and oxidative balance in the cell. Elevated expression of this gene may indicate increased risk of cancer recurrence among breast and ovarian cancer patients. [provided by RefSeq, Sep 2016]

Entrez Gene ID

79094

UniProt ID

Q9BUX1

Alternative Names

ChaC Glutathione Specific Gamma-Glutamylcyclotransferase 1; Gamma-GCT Acting On Glutathione Homolog 1; Blocks Notch Protein; Gamma-GCG 1; Botch; Glutathione-Specific Gamma-Glutamylcyclotransferase 1;

Function

Catalyzes the cleavage of glutathione into 5-oxo-L-proline and a Cys-Gly dipeptide. Acts specifically on glutathione, but not on other gamma-glutamyl peptides (PubMed:27913623).
Glutathione depletion is an important factor for apoptosis initiation and execution. Acts as a pro-apoptotic component of the unfolded protein response pathway by mediating the pro-apoptotic effects of the ATF4-ATF3-DDIT3/CHOP cascade (PubMed:19109178).
Negative regulator of Notch signaling pathway involved in embryonic neurogenesis: acts by inhibiting Notch cleavage by furin, maintaining Notch in an immature inactive form, thereby promoting neurogenesis in embryos (PubMed:22445366).

Biological Process

Glutathione biosynthetic process Source: Reactome
Glutathione catabolic process Source: GO_Central
Intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress Source: UniProtKB
Negative regulation of Notch signaling pathway Source: UniProtKB
Negative regulation of protein processing Source: UniProtKB
Neurogenesis Source: UniProtKB
Notch signaling pathway Source: UniProtKB-KW
Response to unfolded protein Source: UniProtKB-KW

Cellular Location

Cytosol; Trans-Golgi network

He, S., Zhang, M., Ye, Y., Zhuang, J., Ma, X., Song, Y., & Xia, W. (2021). ChaC glutathione specific γ‑glutamylcyclotransferase 1 inhibits cell viability and increases the sensitivity of prostate cancer cells to docetaxel by inducing endoplasmic reticulum stress and ferroptosis. Experimental and therapeutic medicine, 22(3), 1-9.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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