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Rabbit Anti-CHAF1A Recombinant Antibody (CBFYC-1796) (CBMAB-C1858-FY)

This product is rabbit antibody that recognizes CHAF1A. The antibody CBFYC-1796 can be used for immunoassay techniques such as: IF, IP, WB.
See all CHAF1A antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse
Clone
CBFYC-1796
Antibody Isotype
IgG
Application
IF, IP, WB

Basic Information

Immunogen
Synthetic peptide corresponding to residues surrounding Thr293 of human CHAF1A
Specificity
Human, Mouse
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
HEPES, pH 7.5, 150 mM NaCl, 0.1% BSA, 50% glycerol
Preservative
0.02% Sodium azide
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Chromatin Assembly Factor 1 Subunit A
Introduction
Chromatin assembly factor I is a nuclear complex consisting of p50, p60, and p150 subunits that assembles histone octamers onto replicating DNA in vitro.
Entrez Gene ID
Human10036
Mouse27221
UniProt ID
HumanQ13111
MouseQ9QWF0
Alternative Names
Chromatin Assembly Factor 1 Subunit A; Chromatin Assembly Factor I P150 Subunit; Chromatin Assembly Factor I (150 KDa); CAF-I 150 KDa Subunit; CAF-1 Subunit A; CAF-I P150; CAF1P150; Hp150
Function
Core component of the CAF-1 complex, a complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci.
Biological Process
Cell cycle Source: UniProtKB-KW
Chromatin assembly Source: UniProtKB
DNA repair Source: UniProtKB-KW
DNA replication Source: UniProtKB-KW
DNA replication-dependent nucleosome assembly Source: UniProtKB
Nucleosome assembly Source: GO_Central
Cellular Location
Nucleus. DNA replication foci.

Lv, F., Zhong, Y., Sang, L., & Wu, X. (2021). MiR-1179 is downregulated in cervical cancer and its overexpression suppresses cancer cells invasion by targeting CHAF1A/ZEB1. Acta Biochimica Polonica, 68(2), 193-199.

Zhao, H., Duan, Y., Liu, T., Li, L., Li, W., & Li, J. (2019). Over-expression of chromatin assembly factor 1 subunit A (CHAF1A) facilitates cell proliferation in non-small cell lung cancer (NSCLC). Biotechnology & Biotechnological Equipment, 33(1), 894-901.

Zheng, L., Liang, X., Li, S., Li, T., Shang, W., Ma, L., ... & Jia, J. (2018). CHAF1A interacts with TCF4 to promote gastric carcinogenesis via upregulation of c-MYC and CCND1 expression. EBioMedicine, 38, 69-78.

Cai, Y., Dong, Z. Y., & Wang, J. Y. (2018). MiR-520b inhibited metastasis and proliferation of non-small cell lung cancer by targeting CHAF1A. Eur Rev Med Pharmacol Sci, 22(22), 7742-7749.

Xia, D., Yang, X., Liu, W., Shen, F., Pan, J., Lin, Y., ... & Xi, X. (2017). Over-expression of CHAF1A in epithelial ovarian cancer can promote cell proliferation and inhibit cell apoptosis. Biochemical and biophysical research communications, 486(1), 191-197.

Yang, C., Sengupta, S., Hegde, P. M., Mitra, J., Jiang, S., Holey, B., ... & Mitra, S. (2017). Regulation of oxidized base damage repair by chromatin assembly factor 1 subunit A. Nucleic acids research, 45(2), 739-748.

Liu, T., Wei, J., Jiang, C., Wang, C., Zhang, X., Du, Y., ... & Zhao, H. (2017). CHAF1A, the largest subunit of the chromatin assembly factor 1 complex, regulates the growth of H1299 human non‑small cell lung cancer cells by inducing G0/G1 cell cycle arrest. Experimental and therapeutic medicine, 14(5), 4681-4686.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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