Mouse Anti-CHD6 Recombinant Antibody (2174C2a) (CBMAB-C0640-LY)

Name: Mouse Anti-CHD6 Recombinant Antibody (2174C2a)
SKU: CBMAB-C0640-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

2174C2a

Application

WB, IP

Immunogen

C-terminus of CHD6 of human.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.1 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Chromodomain Helicase DNA Binding Protein 6

Introduction

This gene encodes a member of the SNF2/RAD54 helicase protein family. The encoded protein contains two chromodomains, a helicase domain, and an ATPase domain. Several multi-subunit protein complexes remodel chromatin to allow patterns of cell type-specific gene expression, and the encoded protein is thought to be a core member of one or more of these chromatin remodeling complexes. The encoded protein may function as a transcriptional repressor and is involved in the cellular repression of influenza virus replication. [provided by RefSeq, Jul 2013]

Entrez Gene ID

84181

UniProt ID

Q8TD26

Alternative Names

Chromodomain Helicase DNA Binding Protein 6; Radiation-Induced Gene B Protein; ATP-Dependent Helicase CHD6; CHD-6; CHD5; RIGB;

Function

DNA-dependent ATPase that plays a role in chromatin remodeling. Regulates transcription by disrupting nucleosomes in a largely non-sliding manner which strongly increases the accessibility of chromatin (PubMed:28533432).
Activates transcription of specific genes in response to oxidative stress through interaction with NFE2L2.
(Microbial infection) Acts as a transcriptional repressor of different viruses including influenza virus or papillomavirus. During influenza virus infection, the viral polymerase complex localizes CHD6 to inactive chromatin where it gets degraded in a proteasome independent-manner.

Biological Process

Chromatin organization Source: UniProtKB-KW
Positive regulation of transcription from RNA polymerase II promoter in response to oxidative stress Source: UniProtKB
Viral process Source: UniProtKB-KW

Cellular Location

Nucleoplasm. Enriched at sites of mRNA synthesis (PubMed:17027977). During influenza A virus infection, localizes to inactive chromatin.

Involvement in disease

A chromosomal aberration disrupting CHD6 has been found in a patient with mild to moderate mental retardation and minor facial anomalies. Translocation t(18;20)(q21.1;q11.2) with TCF4 producing a CHD6-TCF4 fusion transcript (PubMed:18627065).

Xu, L., & Zheng, Q. (2021). CHD3/4/5/6/8 serve as potential prognostic predictors in human gastric cancer. Network Modeling Analysis in Health Informatics and Bioinformatics, 10(1), 1-12.

Moore, S., Berger, N. D., Luijsterburg, M. S., Piett, C. G., Stanley, F. K., Schräder, C. U., ... & Goodarzi, A. A. (2019). The CHD6 chromatin remodeler is an oxidative DNA damage response factor. Nature communications, 10(1), 1-16.

Manning, B. J., & Yusufzai, T. (2017). The ATP-dependent chromatin remodeling enzymes CHD6, CHD7, and CHD8 exhibit distinct nucleosome binding and remodeling activities. Journal of Biological Chemistry, 292(28), 11927-11936.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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