Mouse Anti-CHDH Recombinant Antibody (1361) (CBMAB-1361-CN)

Name: Mouse Anti-CHDH Recombinant Antibody (1361)
SKU: CBMAB-1361-CN
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

1361

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 575-594 at the C-terminus of CHDH of human.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

Pan

More Infomation

Target

Full Name

Choline Dehydrogenase

Introduction

The protein encoded by this gene is a choline dehydrogenase that localizes to the mitochondrion. Variations in this gene can affect susceptibility to choline deficiency. A few transcript variants have been found for this gene, but the full-length nature of only one has been characterized to date.

Entrez Gene ID

55349

UniProt ID

Q8NE62

Alternative Names

choline dehydrogenase, mitochondrial; CDH; CHD; EC 1.1.99.1

Biological Process

Choline catabolic process Source: Reactome
Glycine betaine biosynthetic process from choline Source: UniProtKB-UniPathway

Cellular Location

Mitochondrion inner membrane

McClatchie, T. (2018). Accumulation of Betaine in the Developing Mouse Oocyte Requires Choline Dehydrogenase (Doctoral dissertation, Université d'Ottawa/University of Ottawa).

McClatchie, T., Meredith, M., Ouédraogo, M. O., Slow, S., Lever, M., Mann, M. R., ... & Baltz, J. M. (2017). Betaine is accumulated via transient choline dehydrogenase activation during mouse oocyte meiotic maturation. Journal of Biological Chemistry, 292(33), 13784-13794.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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